Efficacy of a Standardized Turmeric Extract Comprised of 70% Bisdemothoxy-Curcumin (REVERC3) Against LPS-Induced Inflammation in RAW264.7 Cells and Carrageenan-Induced Paw Edema

Kuluvar Gouthamchandra,1 Heggar Venkataramana Sudeep,1 Siddappa Chandrappa,2 Amrith Raj,1 Puttaswamy Naveen,3 Kodimule Shyamaprasad1 1Department of Biomedicinal Research, R&D Centre for Excellence, Vidya Herbs Pvt. Ltd, Bangalore, Karnataka, 560105, India; 2Department of Phytochemistry, R&am...

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Autores principales:	Gouthamchandra K, Sudeep HV, Chandrappa S, Raj A, Naveen P, Shyamaprasad K
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	lipopolysaccharide nitric oxide xanthine oxidase tnf-α il-6 inos cox2 Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/bffa86f027434e33aa0a5d110638a192
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spelling	oai:doaj.org-article:bffa86f027434e33aa0a5d110638a1922021-12-02T13:31:15ZEfficacy of a Standardized Turmeric Extract Comprised of 70% Bisdemothoxy-Curcumin (REVERC3) Against LPS-Induced Inflammation in RAW264.7 Cells and Carrageenan-Induced Paw Edema1178-7031https://doaj.org/article/bffa86f027434e33aa0a5d110638a1922021-03-01T00:00:00Zhttps://www.dovepress.com/efficacy-of-a-standardized-turmeric-extract-comprised-of-70-bisdemotho-peer-reviewed-article-JIRhttps://doaj.org/toc/1178-7031Kuluvar Gouthamchandra,1 Heggar Venkataramana Sudeep,1 Siddappa Chandrappa,2 Amrith Raj,1 Puttaswamy Naveen,3 Kodimule Shyamaprasad1 1Department of Biomedicinal Research, R&D Centre for Excellence, Vidya Herbs Pvt. Ltd, Bangalore, Karnataka, 560105, India; 2Department of Phytochemistry, R&D Centre for Excellence, Vidya Herbs Pvt. Ltd, Bangalore, Karnataka, 560105, India; 3Department of Analytical Development Research, R&D Centre for Excellence, Vidya Herbs Pvt. Ltd, Bangalore, Karnataka, 560105, IndiaCorrespondence: Kuluvar GouthamchandraDepartment of Biomedicinal Research, R&D Centre for Excellence, Vidya Herbs Pvt. Ltd, #14A, Jigani I Phase, Bangalore, Karnataka, 560105, IndiaTel +91 80 42094158Email goutham06@gmail.comObjective: It is well known that regular turmeric extract with 95% curcuminoid is comprised of curcumin (70.07%), desmethoxycurcumin (20.28%), and bisdemethoxycurcumin (BDMC) (3.63%). In the current study for the first time, we have enriched about 3% of bisdemethoxycurcumin (BDMC) to 70% as well as named it as REVERC3 and compared anti-inflammatory activity with regular turmeric extract using in vitro and in vivo models of inflammation.Methods: To reveal the potential anti-inflammatory mechanism of action, we investigated nitric oxide (NO) scavenging, xanthine oxidase, and lipoxygenase inhibitory activity, further determined the level of pro-inflammatory cytokines, such as interleukin 6 (IL-6), tumor necrosis factor (TNF-α) and major inflammatory mediators like cyclooxygenase (COX-2) and inducible nitric oxide synthase (iNOS), inhibition in lipopolysaccharide (LPS) induced inflammation in RAW macrophage cells. In the other hand, a carrageenan-stimulated inflammatory rat model was carried out.Results: Our study findings exhibited a significant anti-inflammatory activity of REVERC3 together with nitric oxide (NO), xanthine oxidase, and lipoxygenase inhibition. Further, we attenuated the levels of cyclooxygenase (COX-2), inducible nitric oxide synthase (iNOS), interleukin (IL-6) and tumor necrosis factor (TNF-α) expressions in the LPS-elicited RAW macrophage cells. REVERC3 showed a potential anti-inflammatory activity by inhibiting carrageenan induced paw edema after 4 hr at the dose of 100mg/kg body weight.Conclusion: Thus, our findings collectively indicated that the REVERC3 could efficiently inhibit inflammation compared to regular turmeric extract. Since bisdemethoxycurcumin is a stable molecule it could be effectively used in the applications of health care and the nutraceutical industry, indeed which deserves further investigations.Keywords: lipopolysaccharide, nitric oxide, xanthine oxidase, TNF-α, IL-6, iNOS, COX2Gouthamchandra KSudeep HVChandrappa SRaj ANaveen PShyamaprasad KDove Medical Pressarticlelipopolysaccharidenitric oxidexanthine oxidasetnf-αil-6inoscox2PathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 14, Pp 859-868 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	lipopolysaccharide nitric oxide xanthine oxidase tnf-α il-6 inos cox2 Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	lipopolysaccharide nitric oxide xanthine oxidase tnf-α il-6 inos cox2 Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Gouthamchandra K Sudeep HV Chandrappa S Raj A Naveen P Shyamaprasad K Efficacy of a Standardized Turmeric Extract Comprised of 70% Bisdemothoxy-Curcumin (REVERC3) Against LPS-Induced Inflammation in RAW264.7 Cells and Carrageenan-Induced Paw Edema
description	Kuluvar Gouthamchandra,1 Heggar Venkataramana Sudeep,1 Siddappa Chandrappa,2 Amrith Raj,1 Puttaswamy Naveen,3 Kodimule Shyamaprasad1 1Department of Biomedicinal Research, R&D Centre for Excellence, Vidya Herbs Pvt. Ltd, Bangalore, Karnataka, 560105, India; 2Department of Phytochemistry, R&D Centre for Excellence, Vidya Herbs Pvt. Ltd, Bangalore, Karnataka, 560105, India; 3Department of Analytical Development Research, R&D Centre for Excellence, Vidya Herbs Pvt. Ltd, Bangalore, Karnataka, 560105, IndiaCorrespondence: Kuluvar GouthamchandraDepartment of Biomedicinal Research, R&D Centre for Excellence, Vidya Herbs Pvt. Ltd, #14A, Jigani I Phase, Bangalore, Karnataka, 560105, IndiaTel +91 80 42094158Email goutham06@gmail.comObjective: It is well known that regular turmeric extract with 95% curcuminoid is comprised of curcumin (70.07%), desmethoxycurcumin (20.28%), and bisdemethoxycurcumin (BDMC) (3.63%). In the current study for the first time, we have enriched about 3% of bisdemethoxycurcumin (BDMC) to 70% as well as named it as REVERC3 and compared anti-inflammatory activity with regular turmeric extract using in vitro and in vivo models of inflammation.Methods: To reveal the potential anti-inflammatory mechanism of action, we investigated nitric oxide (NO) scavenging, xanthine oxidase, and lipoxygenase inhibitory activity, further determined the level of pro-inflammatory cytokines, such as interleukin 6 (IL-6), tumor necrosis factor (TNF-α) and major inflammatory mediators like cyclooxygenase (COX-2) and inducible nitric oxide synthase (iNOS), inhibition in lipopolysaccharide (LPS) induced inflammation in RAW macrophage cells. In the other hand, a carrageenan-stimulated inflammatory rat model was carried out.Results: Our study findings exhibited a significant anti-inflammatory activity of REVERC3 together with nitric oxide (NO), xanthine oxidase, and lipoxygenase inhibition. Further, we attenuated the levels of cyclooxygenase (COX-2), inducible nitric oxide synthase (iNOS), interleukin (IL-6) and tumor necrosis factor (TNF-α) expressions in the LPS-elicited RAW macrophage cells. REVERC3 showed a potential anti-inflammatory activity by inhibiting carrageenan induced paw edema after 4 hr at the dose of 100mg/kg body weight.Conclusion: Thus, our findings collectively indicated that the REVERC3 could efficiently inhibit inflammation compared to regular turmeric extract. Since bisdemethoxycurcumin is a stable molecule it could be effectively used in the applications of health care and the nutraceutical industry, indeed which deserves further investigations.Keywords: lipopolysaccharide, nitric oxide, xanthine oxidase, TNF-α, IL-6, iNOS, COX2
format	article
author	Gouthamchandra K Sudeep HV Chandrappa S Raj A Naveen P Shyamaprasad K
author_facet	Gouthamchandra K Sudeep HV Chandrappa S Raj A Naveen P Shyamaprasad K
author_sort	Gouthamchandra K
title	Efficacy of a Standardized Turmeric Extract Comprised of 70% Bisdemothoxy-Curcumin (REVERC3) Against LPS-Induced Inflammation in RAW264.7 Cells and Carrageenan-Induced Paw Edema
title_short	Efficacy of a Standardized Turmeric Extract Comprised of 70% Bisdemothoxy-Curcumin (REVERC3) Against LPS-Induced Inflammation in RAW264.7 Cells and Carrageenan-Induced Paw Edema
title_full	Efficacy of a Standardized Turmeric Extract Comprised of 70% Bisdemothoxy-Curcumin (REVERC3) Against LPS-Induced Inflammation in RAW264.7 Cells and Carrageenan-Induced Paw Edema
title_fullStr	Efficacy of a Standardized Turmeric Extract Comprised of 70% Bisdemothoxy-Curcumin (REVERC3) Against LPS-Induced Inflammation in RAW264.7 Cells and Carrageenan-Induced Paw Edema
title_full_unstemmed	Efficacy of a Standardized Turmeric Extract Comprised of 70% Bisdemothoxy-Curcumin (REVERC3) Against LPS-Induced Inflammation in RAW264.7 Cells and Carrageenan-Induced Paw Edema
title_sort	efficacy of a standardized turmeric extract comprised of 70% bisdemothoxy-curcumin (reverc3) against lps-induced inflammation in raw264.7 cells and carrageenan-induced paw edema
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/bffa86f027434e33aa0a5d110638a192
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Efficacy of a Standardized Turmeric Extract Comprised of 70% Bisdemothoxy-Curcumin (REVERC3) Against LPS-Induced Inflammation in RAW264.7 Cells and Carrageenan-Induced Paw Edema

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