Association of NQO1 levels and its genetic polymorphism with susceptibility to methamphetamine dependence

Abstract Background The quinone oxidoreductase 1 (NQO1) gene was involved in the pathophysiological process of illicit drugs abuse, and its polymorphisms might be associated with methamphetamine (METH) dependence susceptibility. The purpose of this study was to examine the NQO1 mRNA and protein leve...

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Autores principales:	Huan Liu, Wei Zhang, Xiao‐Dong Deng, Ying Ma, Yun Liu
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	genetic susceptibility methamphetamine dependence NQO1 SINGLE‐nucleotide polymorphism Genetics QH426-470
Acceso en línea:	https://doaj.org/article/c0115384ac95467a8b00c96c464c4388
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spelling	oai:doaj.org-article:c0115384ac95467a8b00c96c464c43882021-11-10T16:39:23ZAssociation of NQO1 levels and its genetic polymorphism with susceptibility to methamphetamine dependence2324-926910.1002/mgg3.1789https://doaj.org/article/c0115384ac95467a8b00c96c464c43882021-10-01T00:00:00Zhttps://doi.org/10.1002/mgg3.1789https://doaj.org/toc/2324-9269Abstract Background The quinone oxidoreductase 1 (NQO1) gene was involved in the pathophysiological process of illicit drugs abuse, and its polymorphisms might be associated with methamphetamine (METH) dependence susceptibility. The purpose of this study was to examine the NQO1 mRNA and protein levels and to analyze the 609C/T polymorphism (rs1800566) between METH‐dependent patients and controls. Methods A total of 392 METH‐dependent patients (cases) and 669 healthy controls (controls) were enrolled in the study. The quantitative real‐time polymerase chain reaction (RT‐qPCR) and enzyme‐linked immunosorbent assay (ELISA) were used to detect the relative expressions of NQO1 mRNA in PBMCs and protein levels in plasma, respectively. PCR‐restriction fragment length polymorphism (RFLP‐PCR) and direct‐sequencing genotyping were used to detect the alleles and genotypes of NQO1 609C/T polymorphism. Results The levels of NQO1 mRNA in cases (3.2650 ± 2.2943) was significantly higher than in controls (1.0125 ± 0.7959) (p < 0.001), the plasma protein in cases (0.2368 ± 0.1486) was significantly lower than in controls (0.5844 ± 0.1742) (p < 0.001). The T allele of the 609C/T polymorphism significantly increased the risk of METH dependence (p = 0.032, OR = 1.214, 95%CI = 1.017–1.450). The TC and TC/TT genotypes of 609C/T were observed significantly more frequently in cases than in controls, respectively (TC vs CC: p = 0.012, OR = 1.457, 95% CI = 1.087–1.952; TC/TT vs CC: p = 0.008, OR = 1.460, 95% CI = 1.102–1.935). Similar results were obtained after adjusting for age and sex. We failed to find that any genotype of 609C/T polymorphism affected the mRNA or plasma protein levels in controls, respectively (p > 0.05). Conclusion The findings suggested that NQO1 might play an important role in the pathophysiological process of METH dependence, and the 609C/T polymorphism might contribute to the susceptibility to METH dependence in a Chinese Han population.Huan LiuWei ZhangXiao‐Dong DengYing MaYun LiuWileyarticlegenetic susceptibilitymethamphetamine dependenceNQO1SINGLE‐nucleotide polymorphismGeneticsQH426-470ENMolecular Genetics & Genomic Medicine, Vol 9, Iss 10, Pp n/a-n/a (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	genetic susceptibility methamphetamine dependence NQO1 SINGLE‐nucleotide polymorphism Genetics QH426-470
spellingShingle	genetic susceptibility methamphetamine dependence NQO1 SINGLE‐nucleotide polymorphism Genetics QH426-470 Huan Liu Wei Zhang Xiao‐Dong Deng Ying Ma Yun Liu Association of NQO1 levels and its genetic polymorphism with susceptibility to methamphetamine dependence
description	Abstract Background The quinone oxidoreductase 1 (NQO1) gene was involved in the pathophysiological process of illicit drugs abuse, and its polymorphisms might be associated with methamphetamine (METH) dependence susceptibility. The purpose of this study was to examine the NQO1 mRNA and protein levels and to analyze the 609C/T polymorphism (rs1800566) between METH‐dependent patients and controls. Methods A total of 392 METH‐dependent patients (cases) and 669 healthy controls (controls) were enrolled in the study. The quantitative real‐time polymerase chain reaction (RT‐qPCR) and enzyme‐linked immunosorbent assay (ELISA) were used to detect the relative expressions of NQO1 mRNA in PBMCs and protein levels in plasma, respectively. PCR‐restriction fragment length polymorphism (RFLP‐PCR) and direct‐sequencing genotyping were used to detect the alleles and genotypes of NQO1 609C/T polymorphism. Results The levels of NQO1 mRNA in cases (3.2650 ± 2.2943) was significantly higher than in controls (1.0125 ± 0.7959) (p < 0.001), the plasma protein in cases (0.2368 ± 0.1486) was significantly lower than in controls (0.5844 ± 0.1742) (p < 0.001). The T allele of the 609C/T polymorphism significantly increased the risk of METH dependence (p = 0.032, OR = 1.214, 95%CI = 1.017–1.450). The TC and TC/TT genotypes of 609C/T were observed significantly more frequently in cases than in controls, respectively (TC vs CC: p = 0.012, OR = 1.457, 95% CI = 1.087–1.952; TC/TT vs CC: p = 0.008, OR = 1.460, 95% CI = 1.102–1.935). Similar results were obtained after adjusting for age and sex. We failed to find that any genotype of 609C/T polymorphism affected the mRNA or plasma protein levels in controls, respectively (p > 0.05). Conclusion The findings suggested that NQO1 might play an important role in the pathophysiological process of METH dependence, and the 609C/T polymorphism might contribute to the susceptibility to METH dependence in a Chinese Han population.
format	article
author	Huan Liu Wei Zhang Xiao‐Dong Deng Ying Ma Yun Liu
author_facet	Huan Liu Wei Zhang Xiao‐Dong Deng Ying Ma Yun Liu
author_sort	Huan Liu
title	Association of NQO1 levels and its genetic polymorphism with susceptibility to methamphetamine dependence
title_short	Association of NQO1 levels and its genetic polymorphism with susceptibility to methamphetamine dependence
title_full	Association of NQO1 levels and its genetic polymorphism with susceptibility to methamphetamine dependence
title_fullStr	Association of NQO1 levels and its genetic polymorphism with susceptibility to methamphetamine dependence
title_full_unstemmed	Association of NQO1 levels and its genetic polymorphism with susceptibility to methamphetamine dependence
title_sort	association of nqo1 levels and its genetic polymorphism with susceptibility to methamphetamine dependence
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/c0115384ac95467a8b00c96c464c4388
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Association of NQO1 levels and its genetic polymorphism with susceptibility to methamphetamine dependence

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