A molecular detection approach for a cotton aphid-parasitoid complex in northern China
Abstract Aphid-parasitoid interactions have been widely used as a model system in research studies on the structure and functions of arthropod food web. Research on aphid-parasitoid food webs is hindered by their micromorphological characteristics and the high amount of labor associated with their d...
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Nature Portfolio
2019
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oai:doaj.org-article:c0123eb3894a45f19043519ec97d7a7d2021-12-02T15:09:39ZA molecular detection approach for a cotton aphid-parasitoid complex in northern China10.1038/s41598-019-52266-72045-2322https://doaj.org/article/c0123eb3894a45f19043519ec97d7a7d2019-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-52266-7https://doaj.org/toc/2045-2322Abstract Aphid-parasitoid interactions have been widely used as a model system in research studies on the structure and functions of arthropod food web. Research on aphid-parasitoid food webs is hindered by their micromorphological characteristics and the high amount of labor associated with their development. Species-specific primers for cotton aphids and their parasitoids were designed and integrated into two multiplex PCRs and six singleplex PCRs, and all PCRs were optimized to achieve high specificity and sensitivity (100–10,000 DNA copies). One cotton aphid (Aphis gossypii) as well as three primary parasitoid and seven hyperparasitoid species or genera were detected using this molecular approach. This group comprises all the primary parasitoids and 97.2–99.6% of the hyperparasitoids reported in cotton fields in northern China. A tritrophic aphid-primary parasitoid-hyperparasitoid food web was then established. The described method constitutes an efficient tool for quantitatively describing the aphid-primary parasitoid-hyperparasitoid food webs and assessing the efficiency of the biological control of parasitoids in cotton fields in northern China.Yu-Lin ZhuFan YangZhi-Wen YaoYue-Kun WuBing LiuHai-Bin YuanYan-Hui LuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-8 (2019) |
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Medicine R Science Q Yu-Lin Zhu Fan Yang Zhi-Wen Yao Yue-Kun Wu Bing Liu Hai-Bin Yuan Yan-Hui Lu A molecular detection approach for a cotton aphid-parasitoid complex in northern China |
| description |
Abstract Aphid-parasitoid interactions have been widely used as a model system in research studies on the structure and functions of arthropod food web. Research on aphid-parasitoid food webs is hindered by their micromorphological characteristics and the high amount of labor associated with their development. Species-specific primers for cotton aphids and their parasitoids were designed and integrated into two multiplex PCRs and six singleplex PCRs, and all PCRs were optimized to achieve high specificity and sensitivity (100–10,000 DNA copies). One cotton aphid (Aphis gossypii) as well as three primary parasitoid and seven hyperparasitoid species or genera were detected using this molecular approach. This group comprises all the primary parasitoids and 97.2–99.6% of the hyperparasitoids reported in cotton fields in northern China. A tritrophic aphid-primary parasitoid-hyperparasitoid food web was then established. The described method constitutes an efficient tool for quantitatively describing the aphid-primary parasitoid-hyperparasitoid food webs and assessing the efficiency of the biological control of parasitoids in cotton fields in northern China. |
| format |
article |
| author |
Yu-Lin Zhu Fan Yang Zhi-Wen Yao Yue-Kun Wu Bing Liu Hai-Bin Yuan Yan-Hui Lu |
| author_facet |
Yu-Lin Zhu Fan Yang Zhi-Wen Yao Yue-Kun Wu Bing Liu Hai-Bin Yuan Yan-Hui Lu |
| author_sort |
Yu-Lin Zhu |
| title |
A molecular detection approach for a cotton aphid-parasitoid complex in northern China |
| title_short |
A molecular detection approach for a cotton aphid-parasitoid complex in northern China |
| title_full |
A molecular detection approach for a cotton aphid-parasitoid complex in northern China |
| title_fullStr |
A molecular detection approach for a cotton aphid-parasitoid complex in northern China |
| title_full_unstemmed |
A molecular detection approach for a cotton aphid-parasitoid complex in northern China |
| title_sort |
molecular detection approach for a cotton aphid-parasitoid complex in northern china |
| publisher |
Nature Portfolio |
| publishDate |
2019 |
| url |
https://doaj.org/article/c0123eb3894a45f19043519ec97d7a7d |
| work_keys_str_mv |
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