Razvoj metode za HPLC-analizo izbranih fenolnih spojin lesa

The most suitable composition of mobile phase for the separation of simple phenolic compounds such as gallic acid, pyrocatechol, phloroglucinol, resorcinol, vanillin and related compound catechin by the high performance liquid chromatography was investigated. The most appropriate developing...

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Autores principales: Ida Poljanšek, Viljem Vek, Primož Oven
Formato: article
Lenguaje:DE
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SL
Publicado: Slovenian Forestry Institute 2015
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Acceso en línea:https://doaj.org/article/c0142b9b639244f29752b8629cdd9fa0
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Sumario:The most suitable composition of mobile phase for the separation of simple phenolic compounds such as gallic acid, pyrocatechol, phloroglucinol, resorcinol, vanillin and related compound catechin by the high performance liquid chromatography was investigated. The most appropriate developing mobile system for the separation of these organic compounds was acetonitrile and 2% water solution of formic acid with gradient ratio from 2.98 to 25:75. The best separation of the six organic compounds was achieved using mobile phase with lower content of formic acid. HPLC is a method of choice for qualitative and quantitative analysis compounds in various types of samples in the broad field of forestry-wood-pulp-paper chain.