Extraction of human genomic DNA from whole blood using a magnetic microsphere method

Rui Gong,1 Shengying Li2 1Pharmaceutical Department, The Second Hospital of Tianjin Medical University, 2Clinical Laboratory, Tianjin Children's Hospital, Tianjin, People's Republic of China Abstract: With the rapid development of molecular biology and the life sciences, magnetic e...

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Autores principales:	Gong R, Li S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/c014ced30388429d8749ba78a0f74d86
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spelling	oai:doaj.org-article:c014ced30388429d8749ba78a0f74d862021-12-02T05:02:06ZExtraction of human genomic DNA from whole blood using a magnetic microsphere method1178-2013https://doaj.org/article/c014ced30388429d8749ba78a0f74d862014-08-01T00:00:00Zhttp://www.dovepress.com/extraction-of-human-genomic-dna-from-whole-blood-using-a-magnetic-micr-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Rui Gong,1 Shengying Li2 1Pharmaceutical Department, The Second Hospital of Tianjin Medical University, 2Clinical Laboratory, Tianjin Children's Hospital, Tianjin, People's Republic of China Abstract: With the rapid development of molecular biology and the life sciences, magnetic extraction is a simple, automatic, and highly efficient method for separating biological molecules, performing immunoassays, and other applications. Human blood is an ideal source of human genomic DNA. Extracting genomic DNA by traditional methods is time-consuming, and phenol and chloroform are toxic reagents that endanger health. Therefore, it is necessary to find a more convenient and efficient method for obtaining human genomic DNA. In this study, we developed urea–formaldehyde resin magnetic microspheres and magnetic silica microspheres for extraction of human genomic DNA. First, a magnetic microsphere suspension was prepared and used to extract genomic DNA from fresh whole blood, frozen blood, dried blood, and trace blood. Second, DNA content and purity were measured by agarose electrophoresis and ultraviolet spectrophotometry. The human genomic DNA extracted from whole blood was then subjected to polymerase chain reaction analysis to further confirm its quality. The results of this study lay a good foundation for future research and development of a high-throughput and rapid extraction method for extracting genomic DNA from various types of blood samples. Keywords: magnetic separation, bioseparation, resin magnetic microspheres, human genomic DNA, extractionGong RLi SDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 3781-3789 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Gong R Li S Extraction of human genomic DNA from whole blood using a magnetic microsphere method
description	Rui Gong,1 Shengying Li2 1Pharmaceutical Department, The Second Hospital of Tianjin Medical University, 2Clinical Laboratory, Tianjin Children's Hospital, Tianjin, People's Republic of China Abstract: With the rapid development of molecular biology and the life sciences, magnetic extraction is a simple, automatic, and highly efficient method for separating biological molecules, performing immunoassays, and other applications. Human blood is an ideal source of human genomic DNA. Extracting genomic DNA by traditional methods is time-consuming, and phenol and chloroform are toxic reagents that endanger health. Therefore, it is necessary to find a more convenient and efficient method for obtaining human genomic DNA. In this study, we developed urea–formaldehyde resin magnetic microspheres and magnetic silica microspheres for extraction of human genomic DNA. First, a magnetic microsphere suspension was prepared and used to extract genomic DNA from fresh whole blood, frozen blood, dried blood, and trace blood. Second, DNA content and purity were measured by agarose electrophoresis and ultraviolet spectrophotometry. The human genomic DNA extracted from whole blood was then subjected to polymerase chain reaction analysis to further confirm its quality. The results of this study lay a good foundation for future research and development of a high-throughput and rapid extraction method for extracting genomic DNA from various types of blood samples. Keywords: magnetic separation, bioseparation, resin magnetic microspheres, human genomic DNA, extraction
format	article
author	Gong R Li S
author_facet	Gong R Li S
author_sort	Gong R
title	Extraction of human genomic DNA from whole blood using a magnetic microsphere method
title_short	Extraction of human genomic DNA from whole blood using a magnetic microsphere method
title_full	Extraction of human genomic DNA from whole blood using a magnetic microsphere method
title_fullStr	Extraction of human genomic DNA from whole blood using a magnetic microsphere method
title_full_unstemmed	Extraction of human genomic DNA from whole blood using a magnetic microsphere method
title_sort	extraction of human genomic dna from whole blood using a magnetic microsphere method
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/c014ced30388429d8749ba78a0f74d86
work_keys_str_mv	AT gongr extractionofhumangenomicdnafromwholebloodusingamagneticmicrospheremethod AT lis extractionofhumangenomicdnafromwholebloodusingamagneticmicrospheremethod
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Extraction of human genomic DNA from whole blood using a magnetic microsphere method

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