Uremia does not affect neointima formation in mice

Abstract Atherosclerotic cardiovascular disease is a major complication of chronic kidney disease (CKD). CKD leads to uremia, which modulates the phenotype of aortic smooth muscle cells (SMCs). Phenotypic modulation of SMCs plays a key role in accelerating atherosclerosis. We investigated the hypoth...

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Autores principales:	Annemarie Aarup, Carsten H. Nielsen, Line S. Bisgaard, Ilze Bot, Henrik H. El-Ali, Andreas Kjaer, Lars B. Nielsen, Tanja X. Pedersen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c01d0b83ed9c46878998b6f64f53187a
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spelling	oai:doaj.org-article:c01d0b83ed9c46878998b6f64f53187a2021-12-02T16:07:06ZUremia does not affect neointima formation in mice10.1038/s41598-017-06816-62045-2322https://doaj.org/article/c01d0b83ed9c46878998b6f64f53187a2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06816-6https://doaj.org/toc/2045-2322Abstract Atherosclerotic cardiovascular disease is a major complication of chronic kidney disease (CKD). CKD leads to uremia, which modulates the phenotype of aortic smooth muscle cells (SMCs). Phenotypic modulation of SMCs plays a key role in accelerating atherosclerosis. We investigated the hypothesis that uremia potentiates neointima formation in response to vascular injury in mice. Carotid wire injury was performed on C57BL/6 wt and apolipoprotein E knockout (Apoe −/−) mice two weeks after induction of uremia by 5/6 nephrectomy. Wire injury led to neointima formation and downregulation of genes encoding classical SMC markers (i.e., myocardin, α-smooth muscle actin, SM22-alpha, and smooth muscle myosin heavy chain) in both wt and Apoe −/− mice. Contrary to our expectations, uremia did not potentiate neointima formation, nor did it affect intimal lesion composition as judged from magnetic resonance imaging and histological analyses. Also, there was no effect of uremia on SMC marker gene expression in the injured carotid arteries, suggesting that there may be different effects of uremia on SMCs in different vascular beds. In conclusion, uremia does not accelerate neointima formation in response to wire injury of the carotid artery in mice.Annemarie AarupCarsten H. NielsenLine S. BisgaardIlze BotHenrik H. El-AliAndreas KjaerLars B. NielsenTanja X. PedersenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Annemarie Aarup Carsten H. Nielsen Line S. Bisgaard Ilze Bot Henrik H. El-Ali Andreas Kjaer Lars B. Nielsen Tanja X. Pedersen Uremia does not affect neointima formation in mice
description	Abstract Atherosclerotic cardiovascular disease is a major complication of chronic kidney disease (CKD). CKD leads to uremia, which modulates the phenotype of aortic smooth muscle cells (SMCs). Phenotypic modulation of SMCs plays a key role in accelerating atherosclerosis. We investigated the hypothesis that uremia potentiates neointima formation in response to vascular injury in mice. Carotid wire injury was performed on C57BL/6 wt and apolipoprotein E knockout (Apoe −/−) mice two weeks after induction of uremia by 5/6 nephrectomy. Wire injury led to neointima formation and downregulation of genes encoding classical SMC markers (i.e., myocardin, α-smooth muscle actin, SM22-alpha, and smooth muscle myosin heavy chain) in both wt and Apoe −/− mice. Contrary to our expectations, uremia did not potentiate neointima formation, nor did it affect intimal lesion composition as judged from magnetic resonance imaging and histological analyses. Also, there was no effect of uremia on SMC marker gene expression in the injured carotid arteries, suggesting that there may be different effects of uremia on SMCs in different vascular beds. In conclusion, uremia does not accelerate neointima formation in response to wire injury of the carotid artery in mice.
format	article
author	Annemarie Aarup Carsten H. Nielsen Line S. Bisgaard Ilze Bot Henrik H. El-Ali Andreas Kjaer Lars B. Nielsen Tanja X. Pedersen
author_facet	Annemarie Aarup Carsten H. Nielsen Line S. Bisgaard Ilze Bot Henrik H. El-Ali Andreas Kjaer Lars B. Nielsen Tanja X. Pedersen
author_sort	Annemarie Aarup
title	Uremia does not affect neointima formation in mice
title_short	Uremia does not affect neointima formation in mice
title_full	Uremia does not affect neointima formation in mice
title_fullStr	Uremia does not affect neointima formation in mice
title_full_unstemmed	Uremia does not affect neointima formation in mice
title_sort	uremia does not affect neointima formation in mice
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c01d0b83ed9c46878998b6f64f53187a
work_keys_str_mv	AT annemarieaarup uremiadoesnotaffectneointimaformationinmice AT carstenhnielsen uremiadoesnotaffectneointimaformationinmice AT linesbisgaard uremiadoesnotaffectneointimaformationinmice AT ilzebot uremiadoesnotaffectneointimaformationinmice AT henrikhelali uremiadoesnotaffectneointimaformationinmice AT andreaskjaer uremiadoesnotaffectneointimaformationinmice AT larsbnielsen uremiadoesnotaffectneointimaformationinmice AT tanjaxpedersen uremiadoesnotaffectneointimaformationinmice
_version_	1718384742428049408

Uremia does not affect neointima formation in mice

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