Variability of the response of human vaginal Lactobacillus crispatus to 17β-estradiol
Abstract We previously showed that the physiological concentration of 17β-estradiol in the vaginal environment is sufficient to affect the membrane dynamics and adhesion phenotype of the Lactobacillus crispatus strain CIP104459. However, L. crispatus is a heterogeneous species. Here, we investigated...
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2021
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oai:doaj.org-article:c020d28d56b2450e83341457014513692021-12-02T15:56:57ZVariability of the response of human vaginal Lactobacillus crispatus to 17β-estradiol10.1038/s41598-021-91017-52045-2322https://doaj.org/article/c020d28d56b2450e83341457014513692021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91017-5https://doaj.org/toc/2045-2322Abstract We previously showed that the physiological concentration of 17β-estradiol in the vaginal environment is sufficient to affect the membrane dynamics and adhesion phenotype of the Lactobacillus crispatus strain CIP104459. However, L. crispatus is a heterogeneous species. Here, we investigated the effect of 17β-estradiol on the recently isolated L. crispatus vaginal strain V4, related to a cluster distant from CIP104459 and at the limit of being a different subspecies. Grown in the same medium, the two strains expressed a highly similar pool of proteins. However, in contrast to CIP104459, L. crispatus V4 showed high aggregation potential and 17β-estradiol promoted this phenotype. This effect was associated with large changes in cell-surface polarity and Lewis acid/base properties. In addition, we observed no effect on the membrane dynamics, contrary to CIP104459. These results can be explained by differences in the properties and organization of the S layer between the two strains. However, as for CIP104459, 17β-estradiol increased biosurfactant production of L. crispatus V4 and their adhesion to vaginal cells. This suggests that 17β-estradiol agonists would be valuable tools to favor a stable re-implantation of L. crispatus in the vaginal mucosa.Maximilien ClabautAmine M. BoukerbAmine Ben MloukaAmandine SuetAli TahriouiJulien VerdonMagalie BarreauOlivier MaillotAgathe Le TirantMadina KarsybayevaCoralie KremserGérard RedziniakCécile Duclairoir-PocChantal PichonJulie HardouinPascal CosetteSylvie ChevalierMarc G. J. FeuilloleyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021) |
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Medicine R Science Q Maximilien Clabaut Amine M. Boukerb Amine Ben Mlouka Amandine Suet Ali Tahrioui Julien Verdon Magalie Barreau Olivier Maillot Agathe Le Tirant Madina Karsybayeva Coralie Kremser Gérard Redziniak Cécile Duclairoir-Poc Chantal Pichon Julie Hardouin Pascal Cosette Sylvie Chevalier Marc G. J. Feuilloley Variability of the response of human vaginal Lactobacillus crispatus to 17β-estradiol |
| description |
Abstract We previously showed that the physiological concentration of 17β-estradiol in the vaginal environment is sufficient to affect the membrane dynamics and adhesion phenotype of the Lactobacillus crispatus strain CIP104459. However, L. crispatus is a heterogeneous species. Here, we investigated the effect of 17β-estradiol on the recently isolated L. crispatus vaginal strain V4, related to a cluster distant from CIP104459 and at the limit of being a different subspecies. Grown in the same medium, the two strains expressed a highly similar pool of proteins. However, in contrast to CIP104459, L. crispatus V4 showed high aggregation potential and 17β-estradiol promoted this phenotype. This effect was associated with large changes in cell-surface polarity and Lewis acid/base properties. In addition, we observed no effect on the membrane dynamics, contrary to CIP104459. These results can be explained by differences in the properties and organization of the S layer between the two strains. However, as for CIP104459, 17β-estradiol increased biosurfactant production of L. crispatus V4 and their adhesion to vaginal cells. This suggests that 17β-estradiol agonists would be valuable tools to favor a stable re-implantation of L. crispatus in the vaginal mucosa. |
| format |
article |
| author |
Maximilien Clabaut Amine M. Boukerb Amine Ben Mlouka Amandine Suet Ali Tahrioui Julien Verdon Magalie Barreau Olivier Maillot Agathe Le Tirant Madina Karsybayeva Coralie Kremser Gérard Redziniak Cécile Duclairoir-Poc Chantal Pichon Julie Hardouin Pascal Cosette Sylvie Chevalier Marc G. J. Feuilloley |
| author_facet |
Maximilien Clabaut Amine M. Boukerb Amine Ben Mlouka Amandine Suet Ali Tahrioui Julien Verdon Magalie Barreau Olivier Maillot Agathe Le Tirant Madina Karsybayeva Coralie Kremser Gérard Redziniak Cécile Duclairoir-Poc Chantal Pichon Julie Hardouin Pascal Cosette Sylvie Chevalier Marc G. J. Feuilloley |
| author_sort |
Maximilien Clabaut |
| title |
Variability of the response of human vaginal Lactobacillus crispatus to 17β-estradiol |
| title_short |
Variability of the response of human vaginal Lactobacillus crispatus to 17β-estradiol |
| title_full |
Variability of the response of human vaginal Lactobacillus crispatus to 17β-estradiol |
| title_fullStr |
Variability of the response of human vaginal Lactobacillus crispatus to 17β-estradiol |
| title_full_unstemmed |
Variability of the response of human vaginal Lactobacillus crispatus to 17β-estradiol |
| title_sort |
variability of the response of human vaginal lactobacillus crispatus to 17β-estradiol |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/c020d28d56b2450e8334145701451369 |
| work_keys_str_mv |
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