Genome-wide identification and comprehensive analysis of the NAC transcription factor family in sunflower during salt and drought stress

Abstract The NAC (NAM, ATAF1/2, and CUC2), is a large family of plant-specific transcription factors (TFs) that exert crucial regulatory roles in various physiological processes and abiotic stresses. There is scanty information on the role of the NAC family in sunflower (Helianthus annuus L.). In th...

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Autores principales:	Wenhui Li, Youling Zeng, Fangliu Yin, Ran Wei, Xiaofei Mao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c02573b2a7954509b0f6d28c4dfc71c0
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spelling	oai:doaj.org-article:c02573b2a7954509b0f6d28c4dfc71c02021-12-02T18:37:10ZGenome-wide identification and comprehensive analysis of the NAC transcription factor family in sunflower during salt and drought stress10.1038/s41598-021-98107-42045-2322https://doaj.org/article/c02573b2a7954509b0f6d28c4dfc71c02021-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98107-4https://doaj.org/toc/2045-2322Abstract The NAC (NAM, ATAF1/2, and CUC2), is a large family of plant-specific transcription factors (TFs) that exert crucial regulatory roles in various physiological processes and abiotic stresses. There is scanty information on the role of the NAC family in sunflower (Helianthus annuus L.). In this study, we conducted a genome-wide survey and expression analysis of the NAC family in sunflower. A total of 150 HaNACs were identified in sunflower. Phylogenetic analysis to compare HaNACs with Arabidopsis NACs generated 15 clusters. Among them, eight membrane-bound NAC TFs with transmembrane helixes were found (designated as NTLs), which were suggested to be localized in the membrane and transferred to the nucleus through proteolysis. Notably, 12 HaNACs were potentially regulated via miR164 cleavage or translational inhibition. By analyzing RNA-seq data from Sequence Read Archive (SRA), the expression of HaNACs showed tissue specificity and strong response to drought stress. Additionally, phylogenetic analysis of 150 HaNACs with the previously reported NACs related to abiotic stress revealed that 75% of the abiotic stress-related NACs were clustered into the SNAC (abiotic stress-related NAC) group, and only 25% were in the Non-SNAC group. qRT-PCR further demonstrated that about 75% of the HaNACs in the SNAC subgroup were induced by salt and drought stress, and the expression of some HaNACs showed tissue specificity. These findings provide valuable information that can deepen the understanding of how NAC TFs in sunflower respond to abiotic stress.Wenhui LiYouling ZengFangliu YinRan WeiXiaofei MaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wenhui Li Youling Zeng Fangliu Yin Ran Wei Xiaofei Mao Genome-wide identification and comprehensive analysis of the NAC transcription factor family in sunflower during salt and drought stress
description	Abstract The NAC (NAM, ATAF1/2, and CUC2), is a large family of plant-specific transcription factors (TFs) that exert crucial regulatory roles in various physiological processes and abiotic stresses. There is scanty information on the role of the NAC family in sunflower (Helianthus annuus L.). In this study, we conducted a genome-wide survey and expression analysis of the NAC family in sunflower. A total of 150 HaNACs were identified in sunflower. Phylogenetic analysis to compare HaNACs with Arabidopsis NACs generated 15 clusters. Among them, eight membrane-bound NAC TFs with transmembrane helixes were found (designated as NTLs), which were suggested to be localized in the membrane and transferred to the nucleus through proteolysis. Notably, 12 HaNACs were potentially regulated via miR164 cleavage or translational inhibition. By analyzing RNA-seq data from Sequence Read Archive (SRA), the expression of HaNACs showed tissue specificity and strong response to drought stress. Additionally, phylogenetic analysis of 150 HaNACs with the previously reported NACs related to abiotic stress revealed that 75% of the abiotic stress-related NACs were clustered into the SNAC (abiotic stress-related NAC) group, and only 25% were in the Non-SNAC group. qRT-PCR further demonstrated that about 75% of the HaNACs in the SNAC subgroup were induced by salt and drought stress, and the expression of some HaNACs showed tissue specificity. These findings provide valuable information that can deepen the understanding of how NAC TFs in sunflower respond to abiotic stress.
format	article
author	Wenhui Li Youling Zeng Fangliu Yin Ran Wei Xiaofei Mao
author_facet	Wenhui Li Youling Zeng Fangliu Yin Ran Wei Xiaofei Mao
author_sort	Wenhui Li
title	Genome-wide identification and comprehensive analysis of the NAC transcription factor family in sunflower during salt and drought stress
title_short	Genome-wide identification and comprehensive analysis of the NAC transcription factor family in sunflower during salt and drought stress
title_full	Genome-wide identification and comprehensive analysis of the NAC transcription factor family in sunflower during salt and drought stress
title_fullStr	Genome-wide identification and comprehensive analysis of the NAC transcription factor family in sunflower during salt and drought stress
title_full_unstemmed	Genome-wide identification and comprehensive analysis of the NAC transcription factor family in sunflower during salt and drought stress
title_sort	genome-wide identification and comprehensive analysis of the nac transcription factor family in sunflower during salt and drought stress
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c02573b2a7954509b0f6d28c4dfc71c0
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Genome-wide identification and comprehensive analysis of the NAC transcription factor family in sunflower during salt and drought stress

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