Modulation of mouse embryonic stem cell proliferation and neural differentiation by the P2X7 receptor.

<h4>Background</h4>Novel developmental functions have been attributed to the P2X7 receptor (P2X7R) including proliferation stimulation and neural differentiation. Mouse embryonic stem cells (ESC), induced with retinoic acid to neural differentiation, closely assemble processes occurring...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Talita Glaser, Sophia La Banca de Oliveira, Arquimedes Cheffer, Renata Beco, Patrícia Martins, Maynara Fornazari, Claudiana Lameu, Helio Miranda Costa Junior, Robson Coutinho-Silva, Henning Ulrich
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c025ea30d9a64a098e97483c9e8293de
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c025ea30d9a64a098e97483c9e8293de
record_format	dspace
spelling	oai:doaj.org-article:c025ea30d9a64a098e97483c9e8293de2021-11-18T08:20:45ZModulation of mouse embryonic stem cell proliferation and neural differentiation by the P2X7 receptor.1932-620310.1371/journal.pone.0096281https://doaj.org/article/c025ea30d9a64a098e97483c9e8293de2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24798220/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Novel developmental functions have been attributed to the P2X7 receptor (P2X7R) including proliferation stimulation and neural differentiation. Mouse embryonic stem cells (ESC), induced with retinoic acid to neural differentiation, closely assemble processes occurring during neuroectodermal development of the early embryo.<h4>Principal findings</h4>P2X7R expression together with the pluripotency marker Oct-4 was highest in undifferentiated ESC. In undifferentiated cells, the P2X7R agonist Bz-ATP accelerated cell cycle entry, which was blocked by the specific P2X7R inhibitor KN-62. ESC induced to neural differentiation with retinoic acid, reduced Oct-4 and P2X7R expression. P2X7R receptor-promoted intracellular calcium fluxes were obtained at lower Bz-ATP ligand concentrations in undifferentiated and in neural-differentiated cells compared to other studies. The presence of KN-62 led to increased number of cells expressing SSEA-1, Dcx and β3-tubulin, as well as the number of SSEA-1 and β3-tubulin-double-positive cells confirming that onset of neuroectodermal differentiation and neuronal fate determination depends on suppression of P2X7R activity. Moreover, an increase in the number of Ki-67 positive cells in conditions of P2X7R inhibition indicates rescue of progenitors into the cell cycle, augmenting the number of neuroblasts and consequently neurogenesis.<h4>Conclusions</h4>In embryonic cells, P2X7R expression and activity is upregulated, maintaining proliferation, while upon induction to neural differentiation P2X7 receptor expression and activity needs to be suppressed.Talita GlaserSophia La Banca de OliveiraArquimedes ChefferRenata BecoPatrícia MartinsMaynara FornazariClaudiana LameuHelio Miranda Costa JuniorRobson Coutinho-SilvaHenning UlrichPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 5, p e96281 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Talita Glaser Sophia La Banca de Oliveira Arquimedes Cheffer Renata Beco Patrícia Martins Maynara Fornazari Claudiana Lameu Helio Miranda Costa Junior Robson Coutinho-Silva Henning Ulrich Modulation of mouse embryonic stem cell proliferation and neural differentiation by the P2X7 receptor.
description	<h4>Background</h4>Novel developmental functions have been attributed to the P2X7 receptor (P2X7R) including proliferation stimulation and neural differentiation. Mouse embryonic stem cells (ESC), induced with retinoic acid to neural differentiation, closely assemble processes occurring during neuroectodermal development of the early embryo.<h4>Principal findings</h4>P2X7R expression together with the pluripotency marker Oct-4 was highest in undifferentiated ESC. In undifferentiated cells, the P2X7R agonist Bz-ATP accelerated cell cycle entry, which was blocked by the specific P2X7R inhibitor KN-62. ESC induced to neural differentiation with retinoic acid, reduced Oct-4 and P2X7R expression. P2X7R receptor-promoted intracellular calcium fluxes were obtained at lower Bz-ATP ligand concentrations in undifferentiated and in neural-differentiated cells compared to other studies. The presence of KN-62 led to increased number of cells expressing SSEA-1, Dcx and β3-tubulin, as well as the number of SSEA-1 and β3-tubulin-double-positive cells confirming that onset of neuroectodermal differentiation and neuronal fate determination depends on suppression of P2X7R activity. Moreover, an increase in the number of Ki-67 positive cells in conditions of P2X7R inhibition indicates rescue of progenitors into the cell cycle, augmenting the number of neuroblasts and consequently neurogenesis.<h4>Conclusions</h4>In embryonic cells, P2X7R expression and activity is upregulated, maintaining proliferation, while upon induction to neural differentiation P2X7 receptor expression and activity needs to be suppressed.
format	article
author	Talita Glaser Sophia La Banca de Oliveira Arquimedes Cheffer Renata Beco Patrícia Martins Maynara Fornazari Claudiana Lameu Helio Miranda Costa Junior Robson Coutinho-Silva Henning Ulrich
author_facet	Talita Glaser Sophia La Banca de Oliveira Arquimedes Cheffer Renata Beco Patrícia Martins Maynara Fornazari Claudiana Lameu Helio Miranda Costa Junior Robson Coutinho-Silva Henning Ulrich
author_sort	Talita Glaser
title	Modulation of mouse embryonic stem cell proliferation and neural differentiation by the P2X7 receptor.
title_short	Modulation of mouse embryonic stem cell proliferation and neural differentiation by the P2X7 receptor.
title_full	Modulation of mouse embryonic stem cell proliferation and neural differentiation by the P2X7 receptor.
title_fullStr	Modulation of mouse embryonic stem cell proliferation and neural differentiation by the P2X7 receptor.
title_full_unstemmed	Modulation of mouse embryonic stem cell proliferation and neural differentiation by the P2X7 receptor.
title_sort	modulation of mouse embryonic stem cell proliferation and neural differentiation by the p2x7 receptor.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/c025ea30d9a64a098e97483c9e8293de
work_keys_str_mv	AT talitaglaser modulationofmouseembryonicstemcellproliferationandneuraldifferentiationbythep2x7receptor AT sophialabancadeoliveira modulationofmouseembryonicstemcellproliferationandneuraldifferentiationbythep2x7receptor AT arquimedescheffer modulationofmouseembryonicstemcellproliferationandneuraldifferentiationbythep2x7receptor AT renatabeco modulationofmouseembryonicstemcellproliferationandneuraldifferentiationbythep2x7receptor AT patriciamartins modulationofmouseembryonicstemcellproliferationandneuraldifferentiationbythep2x7receptor AT maynarafornazari modulationofmouseembryonicstemcellproliferationandneuraldifferentiationbythep2x7receptor AT claudianalameu modulationofmouseembryonicstemcellproliferationandneuraldifferentiationbythep2x7receptor AT heliomirandacostajunior modulationofmouseembryonicstemcellproliferationandneuraldifferentiationbythep2x7receptor AT robsoncoutinhosilva modulationofmouseembryonicstemcellproliferationandneuraldifferentiationbythep2x7receptor AT henningulrich modulationofmouseembryonicstemcellproliferationandneuraldifferentiationbythep2x7receptor
_version_	1718421886582390784

Modulation of mouse embryonic stem cell proliferation and neural differentiation by the P2X7 receptor.

Ejemplares similares