Complete structural model of Escherichia coli RNA polymerase from a hybrid approach.

The Escherichia coli transcription system is the best characterized from a biochemical and genetic point of view and has served as a model system. Nevertheless, a molecular understanding of the details of E. coli transcription and its regulation, and therefore its full exploitation as a model system...

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Autores principales:	Natacha Opalka, Jesse Brown, William J Lane, Kelly-Anne F Twist, Robert Landick, Francisco J Asturias, Seth A Darst
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/c02ae16cbc07446e81ced5aaa6ec2ae6
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spelling	oai:doaj.org-article:c02ae16cbc07446e81ced5aaa6ec2ae62021-11-18T05:34:51ZComplete structural model of Escherichia coli RNA polymerase from a hybrid approach.1544-91731545-788510.1371/journal.pbio.1000483https://doaj.org/article/c02ae16cbc07446e81ced5aaa6ec2ae62010-09-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20856905/pdf/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885The Escherichia coli transcription system is the best characterized from a biochemical and genetic point of view and has served as a model system. Nevertheless, a molecular understanding of the details of E. coli transcription and its regulation, and therefore its full exploitation as a model system, has been hampered by the absence of high-resolution structural information on E. coli RNA polymerase (RNAP). We use a combination of approaches, including high-resolution X-ray crystallography, ab initio structural prediction, homology modeling, and single-particle cryo-electron microscopy, to generate complete atomic models of E. coli core RNAP and an E. coli RNAP ternary elongation complex. The detailed and comprehensive structural descriptions can be used to help interpret previous biochemical and genetic data in a new light and provide a structural framework for designing experiments to understand the function of the E. coli lineage-specific insertions and their role in the E. coli transcription program.Natacha OpalkaJesse BrownWilliam J LaneKelly-Anne F TwistRobert LandickFrancisco J AsturiasSeth A DarstPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 8, Iss 9 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Natacha Opalka Jesse Brown William J Lane Kelly-Anne F Twist Robert Landick Francisco J Asturias Seth A Darst Complete structural model of Escherichia coli RNA polymerase from a hybrid approach.
description	The Escherichia coli transcription system is the best characterized from a biochemical and genetic point of view and has served as a model system. Nevertheless, a molecular understanding of the details of E. coli transcription and its regulation, and therefore its full exploitation as a model system, has been hampered by the absence of high-resolution structural information on E. coli RNA polymerase (RNAP). We use a combination of approaches, including high-resolution X-ray crystallography, ab initio structural prediction, homology modeling, and single-particle cryo-electron microscopy, to generate complete atomic models of E. coli core RNAP and an E. coli RNAP ternary elongation complex. The detailed and comprehensive structural descriptions can be used to help interpret previous biochemical and genetic data in a new light and provide a structural framework for designing experiments to understand the function of the E. coli lineage-specific insertions and their role in the E. coli transcription program.
format	article
author	Natacha Opalka Jesse Brown William J Lane Kelly-Anne F Twist Robert Landick Francisco J Asturias Seth A Darst
author_facet	Natacha Opalka Jesse Brown William J Lane Kelly-Anne F Twist Robert Landick Francisco J Asturias Seth A Darst
author_sort	Natacha Opalka
title	Complete structural model of Escherichia coli RNA polymerase from a hybrid approach.
title_short	Complete structural model of Escherichia coli RNA polymerase from a hybrid approach.
title_full	Complete structural model of Escherichia coli RNA polymerase from a hybrid approach.
title_fullStr	Complete structural model of Escherichia coli RNA polymerase from a hybrid approach.
title_full_unstemmed	Complete structural model of Escherichia coli RNA polymerase from a hybrid approach.
title_sort	complete structural model of escherichia coli rna polymerase from a hybrid approach.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/c02ae16cbc07446e81ced5aaa6ec2ae6
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Complete structural model of Escherichia coli RNA polymerase from a hybrid approach.

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