Plasmodium berghei P47 is essential for ookinete protection from the Anopheles gambiae complement-like response

Abstract Malaria is a mosquito-borne disease affecting millions of people every year. The rodent parasite Plasmodium berghei has served as a model for human malaria transmission studies and played a pivotal role in dissecting the mosquito immune response against infection. The 6-cysteine protein P47...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Chiamaka Valerie Ukegbu, Maria Giorgalli, Hassan Yassine, Jose Luis Ramirez, Chrysanthi Taxiarchi, Carolina Barillas-Mury, George K. Christophides, Dina Vlachou
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c035f05e477f48ec99621db1c9d2917f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c035f05e477f48ec99621db1c9d2917f
record_format	dspace
spelling	oai:doaj.org-article:c035f05e477f48ec99621db1c9d2917f2021-12-02T16:07:06ZPlasmodium berghei P47 is essential for ookinete protection from the Anopheles gambiae complement-like response10.1038/s41598-017-05917-62045-2322https://doaj.org/article/c035f05e477f48ec99621db1c9d2917f2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05917-6https://doaj.org/toc/2045-2322Abstract Malaria is a mosquito-borne disease affecting millions of people every year. The rodent parasite Plasmodium berghei has served as a model for human malaria transmission studies and played a pivotal role in dissecting the mosquito immune response against infection. The 6-cysteine protein P47, known to be important for P. berghei female gamete fertility, is shown to serve a different function in Plasmodium falciparum, protecting ookinetes from the mosquito immune response. Here, we investigate the function of P. berghei P47 in Anopheles gambiae mosquito infections. We show that P47 is expressed on the surface of both female gametocytes and ookinetes where it serves distinct functions in promoting gametocyte-to-ookinete development and protecting ookinetes from the mosquito complement-like response, respectively. The latter function is essential, as ookinetes lacking P47 are targeted for killing while traversing the mosquito midgut cells and eliminated upon exposure to hemolymph proteins of the complement-like system. Silencing key factors of the complement-like system restores oocyst development and disease transmission to rodent hosts. Our data establish a dual role of P. berghei P47 in vivo and reinforce the use of this parasite to study the impact of the mosquito immune response on human malaria transmission.Chiamaka Valerie UkegbuMaria GiorgalliHassan YassineJose Luis RamirezChrysanthi TaxiarchiCarolina Barillas-MuryGeorge K. ChristophidesDina VlachouNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chiamaka Valerie Ukegbu Maria Giorgalli Hassan Yassine Jose Luis Ramirez Chrysanthi Taxiarchi Carolina Barillas-Mury George K. Christophides Dina Vlachou Plasmodium berghei P47 is essential for ookinete protection from the Anopheles gambiae complement-like response
description	Abstract Malaria is a mosquito-borne disease affecting millions of people every year. The rodent parasite Plasmodium berghei has served as a model for human malaria transmission studies and played a pivotal role in dissecting the mosquito immune response against infection. The 6-cysteine protein P47, known to be important for P. berghei female gamete fertility, is shown to serve a different function in Plasmodium falciparum, protecting ookinetes from the mosquito immune response. Here, we investigate the function of P. berghei P47 in Anopheles gambiae mosquito infections. We show that P47 is expressed on the surface of both female gametocytes and ookinetes where it serves distinct functions in promoting gametocyte-to-ookinete development and protecting ookinetes from the mosquito complement-like response, respectively. The latter function is essential, as ookinetes lacking P47 are targeted for killing while traversing the mosquito midgut cells and eliminated upon exposure to hemolymph proteins of the complement-like system. Silencing key factors of the complement-like system restores oocyst development and disease transmission to rodent hosts. Our data establish a dual role of P. berghei P47 in vivo and reinforce the use of this parasite to study the impact of the mosquito immune response on human malaria transmission.
format	article
author	Chiamaka Valerie Ukegbu Maria Giorgalli Hassan Yassine Jose Luis Ramirez Chrysanthi Taxiarchi Carolina Barillas-Mury George K. Christophides Dina Vlachou
author_facet	Chiamaka Valerie Ukegbu Maria Giorgalli Hassan Yassine Jose Luis Ramirez Chrysanthi Taxiarchi Carolina Barillas-Mury George K. Christophides Dina Vlachou
author_sort	Chiamaka Valerie Ukegbu
title	Plasmodium berghei P47 is essential for ookinete protection from the Anopheles gambiae complement-like response
title_short	Plasmodium berghei P47 is essential for ookinete protection from the Anopheles gambiae complement-like response
title_full	Plasmodium berghei P47 is essential for ookinete protection from the Anopheles gambiae complement-like response
title_fullStr	Plasmodium berghei P47 is essential for ookinete protection from the Anopheles gambiae complement-like response
title_full_unstemmed	Plasmodium berghei P47 is essential for ookinete protection from the Anopheles gambiae complement-like response
title_sort	plasmodium berghei p47 is essential for ookinete protection from the anopheles gambiae complement-like response
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c035f05e477f48ec99621db1c9d2917f
work_keys_str_mv	AT chiamakavalerieukegbu plasmodiumbergheip47isessentialforookineteprotectionfromtheanophelesgambiaecomplementlikeresponse AT mariagiorgalli plasmodiumbergheip47isessentialforookineteprotectionfromtheanophelesgambiaecomplementlikeresponse AT hassanyassine plasmodiumbergheip47isessentialforookineteprotectionfromtheanophelesgambiaecomplementlikeresponse AT joseluisramirez plasmodiumbergheip47isessentialforookineteprotectionfromtheanophelesgambiaecomplementlikeresponse AT chrysanthitaxiarchi plasmodiumbergheip47isessentialforookineteprotectionfromtheanophelesgambiaecomplementlikeresponse AT carolinabarillasmury plasmodiumbergheip47isessentialforookineteprotectionfromtheanophelesgambiaecomplementlikeresponse AT georgekchristophides plasmodiumbergheip47isessentialforookineteprotectionfromtheanophelesgambiaecomplementlikeresponse AT dinavlachou plasmodiumbergheip47isessentialforookineteprotectionfromtheanophelesgambiaecomplementlikeresponse
_version_	1718384742613647360

Plasmodium berghei P47 is essential for ookinete protection from the Anopheles gambiae complement-like response

Ejemplares similares