LncRNAs Associated with Chemoradiotherapy Response and Prognosis in Locally Advanced Rectal Cancer

Yiyi Zhang,1,&ast; Bingjie Guan,2,&ast; Yong WU,1,&ast; Fan Du,3,&ast; Jinfu Zhuang,1 Yuanfeng Yang,1 Guoxian Guan,1 Xing Liu1 1Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, People’s Republic of China; 2Department of Radiation O...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zhang Y, Guan B, WU Y, Du F, Zhuang J, Yang Y, Guan G, Liu X
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	rectal cancer chemoradiotherapy lncrna prognosis Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/c037ff03ff674a6093c4e2996bd0f29e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c037ff03ff674a6093c4e2996bd0f29e
record_format	dspace
spelling	oai:doaj.org-article:c037ff03ff674a6093c4e2996bd0f29e2021-11-28T19:13:10ZLncRNAs Associated with Chemoradiotherapy Response and Prognosis in Locally Advanced Rectal Cancer1178-7031https://doaj.org/article/c037ff03ff674a6093c4e2996bd0f29e2021-11-01T00:00:00Zhttps://www.dovepress.com/lncrnas-associated-with-chemoradiotherapy-response-and-prognosis-in-lo-peer-reviewed-fulltext-article-JIRhttps://doaj.org/toc/1178-7031Yiyi Zhang,1,&ast; Bingjie Guan,2,&ast; Yong WU,1,&ast; Fan Du,3,&ast; Jinfu Zhuang,1 Yuanfeng Yang,1 Guoxian Guan,1 Xing Liu1 1Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, People’s Republic of China; 2Department of Radiation Oncology, Fujian Medical University Union Hospital, Fuzhou, People’s Republic of China; 3Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 330006, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Xing Liu; Guoxian GuanDepartment of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, 20 Chazhong Road, Fuzhou City, 350001, Fujian, People’s Republic of ChinaEmail fjmufylx@163.com; fjxhggx@163.comBackground: There are only limited studies on the long non-coding RNAs (lncRNAs) associated with neoadjuvant chemoradiotherapy (NCRT) response and prognosis of locally advanced rectal cancer (LARC) patients. This study identified lncRNAs associated with NCRT response and prognosis in CRC patients and explored their potential predictive mechanisms.Methods: The study subjected the LncRNA expression profiles from our previous gene chip data to LASSO and identified a four-lncRNA signature that predicted NCRT response and prognosis. A Cox regression model was subsequently performed to identify the prognostic risk factors. The function of LINC00909, the lncRNA with the most powerful predictive ability, was finally identified in vivo and in vitro using CRC cell lines.Results: A comparison of the relative lncRNA expression of NCRT-responsive and non-responsive patients revealed four hub lncRNAs: DBET, LINC00909, FLJ33534, and HSD52 with AUC = 0.68, 0.73, 0.73, and 0.70, respectively (all p < 0.05). COX regression analysis further demonstrated that DBET, LINC00909 and FLJ33534 were associated with the DFS in CRC patients. The expression of the four lncRNAs was also significant in LARC patients who had not undergone NCRT (all p < 0.05). A risk score model was subsequently constructed based on the results of the multivariate COX analysis and used to predict NCRT response and prognosis in the CRC and LARC patients. The expression and prognosis of DBET, LINC00909 and FLJ33534 in the CRC tissues were further validated in the R2 platform and Oncomine database. Notably, overexpression of the LINC00909 increased the cell line resistance to the 5-FU and radiotherapy in vivo and in vitro.Conclusion: DBET, LINC00909, and FLJ33534 are potential novel biomarkers for predicting NCRT response and prognosis in CRC patients. In particular, LINC00909 is an effective oncogene in CRC that could be used as a novel therapeutic target to enhance NCRT response.Keywords: rectal cancer, chemoradiotherapy, lncRNA, prognosisZhang YGuan BWU YDu FZhuang JYang YGuan GLiu XDove Medical Pressarticlerectal cancerchemoradiotherapylncrnaprognosisPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 14, Pp 6275-6292 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	rectal cancer chemoradiotherapy lncrna prognosis Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	rectal cancer chemoradiotherapy lncrna prognosis Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Zhang Y Guan B WU Y Du F Zhuang J Yang Y Guan G Liu X LncRNAs Associated with Chemoradiotherapy Response and Prognosis in Locally Advanced Rectal Cancer
description	Yiyi Zhang,1,&ast; Bingjie Guan,2,&ast; Yong WU,1,&ast; Fan Du,3,&ast; Jinfu Zhuang,1 Yuanfeng Yang,1 Guoxian Guan,1 Xing Liu1 1Department of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, People’s Republic of China; 2Department of Radiation Oncology, Fujian Medical University Union Hospital, Fuzhou, People’s Republic of China; 3Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 330006, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Xing Liu; Guoxian GuanDepartment of Colorectal Surgery, The First Affiliated Hospital of Fujian Medical University, 20 Chazhong Road, Fuzhou City, 350001, Fujian, People’s Republic of ChinaEmail fjmufylx@163.com; fjxhggx@163.comBackground: There are only limited studies on the long non-coding RNAs (lncRNAs) associated with neoadjuvant chemoradiotherapy (NCRT) response and prognosis of locally advanced rectal cancer (LARC) patients. This study identified lncRNAs associated with NCRT response and prognosis in CRC patients and explored their potential predictive mechanisms.Methods: The study subjected the LncRNA expression profiles from our previous gene chip data to LASSO and identified a four-lncRNA signature that predicted NCRT response and prognosis. A Cox regression model was subsequently performed to identify the prognostic risk factors. The function of LINC00909, the lncRNA with the most powerful predictive ability, was finally identified in vivo and in vitro using CRC cell lines.Results: A comparison of the relative lncRNA expression of NCRT-responsive and non-responsive patients revealed four hub lncRNAs: DBET, LINC00909, FLJ33534, and HSD52 with AUC = 0.68, 0.73, 0.73, and 0.70, respectively (all p < 0.05). COX regression analysis further demonstrated that DBET, LINC00909 and FLJ33534 were associated with the DFS in CRC patients. The expression of the four lncRNAs was also significant in LARC patients who had not undergone NCRT (all p < 0.05). A risk score model was subsequently constructed based on the results of the multivariate COX analysis and used to predict NCRT response and prognosis in the CRC and LARC patients. The expression and prognosis of DBET, LINC00909 and FLJ33534 in the CRC tissues were further validated in the R2 platform and Oncomine database. Notably, overexpression of the LINC00909 increased the cell line resistance to the 5-FU and radiotherapy in vivo and in vitro.Conclusion: DBET, LINC00909, and FLJ33534 are potential novel biomarkers for predicting NCRT response and prognosis in CRC patients. In particular, LINC00909 is an effective oncogene in CRC that could be used as a novel therapeutic target to enhance NCRT response.Keywords: rectal cancer, chemoradiotherapy, lncRNA, prognosis
format	article
author	Zhang Y Guan B WU Y Du F Zhuang J Yang Y Guan G Liu X
author_facet	Zhang Y Guan B WU Y Du F Zhuang J Yang Y Guan G Liu X
author_sort	Zhang Y
title	LncRNAs Associated with Chemoradiotherapy Response and Prognosis in Locally Advanced Rectal Cancer
title_short	LncRNAs Associated with Chemoradiotherapy Response and Prognosis in Locally Advanced Rectal Cancer
title_full	LncRNAs Associated with Chemoradiotherapy Response and Prognosis in Locally Advanced Rectal Cancer
title_fullStr	LncRNAs Associated with Chemoradiotherapy Response and Prognosis in Locally Advanced Rectal Cancer
title_full_unstemmed	LncRNAs Associated with Chemoradiotherapy Response and Prognosis in Locally Advanced Rectal Cancer
title_sort	lncrnas associated with chemoradiotherapy response and prognosis in locally advanced rectal cancer
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/c037ff03ff674a6093c4e2996bd0f29e
work_keys_str_mv	AT zhangy lncrnasassociatedwithchemoradiotherapyresponseandprognosisinlocallyadvancedrectalcancer AT guanb lncrnasassociatedwithchemoradiotherapyresponseandprognosisinlocallyadvancedrectalcancer AT wuy lncrnasassociatedwithchemoradiotherapyresponseandprognosisinlocallyadvancedrectalcancer AT duf lncrnasassociatedwithchemoradiotherapyresponseandprognosisinlocallyadvancedrectalcancer AT zhuangj lncrnasassociatedwithchemoradiotherapyresponseandprognosisinlocallyadvancedrectalcancer AT yangy lncrnasassociatedwithchemoradiotherapyresponseandprognosisinlocallyadvancedrectalcancer AT guang lncrnasassociatedwithchemoradiotherapyresponseandprognosisinlocallyadvancedrectalcancer AT liux lncrnasassociatedwithchemoradiotherapyresponseandprognosisinlocallyadvancedrectalcancer
_version_	1718407795284377600

LncRNAs Associated with Chemoradiotherapy Response and Prognosis in Locally Advanced Rectal Cancer

Ejemplares similares