Cell-cycle analysis of fission yeast cells by flow cytometry.

The cell cycle of the fission yeast, Schizosaccharomyces pombe, does not easily lend itself to analysis by flow cytometry, mainly because cells in G(1) and G(2) phase contain the same amount of DNA. This occurs because fission yeast cells under standard growth conditions do not complete cytokinesis...

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Autores principales:	Jon Halvor Jonsrud Knutsen, Idun Dale Rein, Christiane Rothe, Trond Stokke, Beáta Grallert, Erik Boye
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c03b9dae075b45529f13719fcdb2d898
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spelling	oai:doaj.org-article:c03b9dae075b45529f13719fcdb2d8982021-11-18T06:58:07ZCell-cycle analysis of fission yeast cells by flow cytometry.1932-620310.1371/journal.pone.0017175https://doaj.org/article/c03b9dae075b45529f13719fcdb2d8982011-02-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21386895/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The cell cycle of the fission yeast, Schizosaccharomyces pombe, does not easily lend itself to analysis by flow cytometry, mainly because cells in G(1) and G(2) phase contain the same amount of DNA. This occurs because fission yeast cells under standard growth conditions do not complete cytokinesis until after G(1) phase. We have devised a flow cytometric method exploiting the fact that cells in G(1) phase contain two nuclei, whereas cells in G(2) are mononuclear. Measurements of the width as well as the total area of the DNA-associated fluorescence signal allows the discrimination between cells in G(1) and in G(2) phase and the cell-cycle progression of fission yeast can be followed in detail by flow cytometry. Furthermore, we show how this method can be used to monitor the timing of cell entry into anaphase. Fission yeast cells tend to form multimers, which represents another problem of flow cytometry-based cell-cycle analysis. Here we present a method employing light-scatter measurements to enable the exclusion of cell doublets, thereby further improving the analysis of fission yeast cells by flow cytometry.Jon Halvor Jonsrud KnutsenIdun Dale ReinChristiane RotheTrond StokkeBeáta GrallertErik BoyePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 2, p e17175 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jon Halvor Jonsrud Knutsen Idun Dale Rein Christiane Rothe Trond Stokke Beáta Grallert Erik Boye Cell-cycle analysis of fission yeast cells by flow cytometry.
description	The cell cycle of the fission yeast, Schizosaccharomyces pombe, does not easily lend itself to analysis by flow cytometry, mainly because cells in G(1) and G(2) phase contain the same amount of DNA. This occurs because fission yeast cells under standard growth conditions do not complete cytokinesis until after G(1) phase. We have devised a flow cytometric method exploiting the fact that cells in G(1) phase contain two nuclei, whereas cells in G(2) are mononuclear. Measurements of the width as well as the total area of the DNA-associated fluorescence signal allows the discrimination between cells in G(1) and in G(2) phase and the cell-cycle progression of fission yeast can be followed in detail by flow cytometry. Furthermore, we show how this method can be used to monitor the timing of cell entry into anaphase. Fission yeast cells tend to form multimers, which represents another problem of flow cytometry-based cell-cycle analysis. Here we present a method employing light-scatter measurements to enable the exclusion of cell doublets, thereby further improving the analysis of fission yeast cells by flow cytometry.
format	article
author	Jon Halvor Jonsrud Knutsen Idun Dale Rein Christiane Rothe Trond Stokke Beáta Grallert Erik Boye
author_facet	Jon Halvor Jonsrud Knutsen Idun Dale Rein Christiane Rothe Trond Stokke Beáta Grallert Erik Boye
author_sort	Jon Halvor Jonsrud Knutsen
title	Cell-cycle analysis of fission yeast cells by flow cytometry.
title_short	Cell-cycle analysis of fission yeast cells by flow cytometry.
title_full	Cell-cycle analysis of fission yeast cells by flow cytometry.
title_fullStr	Cell-cycle analysis of fission yeast cells by flow cytometry.
title_full_unstemmed	Cell-cycle analysis of fission yeast cells by flow cytometry.
title_sort	cell-cycle analysis of fission yeast cells by flow cytometry.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/c03b9dae075b45529f13719fcdb2d898
work_keys_str_mv	AT jonhalvorjonsrudknutsen cellcycleanalysisoffissionyeastcellsbyflowcytometry AT idundalerein cellcycleanalysisoffissionyeastcellsbyflowcytometry AT christianerothe cellcycleanalysisoffissionyeastcellsbyflowcytometry AT trondstokke cellcycleanalysisoffissionyeastcellsbyflowcytometry AT beatagrallert cellcycleanalysisoffissionyeastcellsbyflowcytometry AT erikboye cellcycleanalysisoffissionyeastcellsbyflowcytometry
_version_	1718424131738796032

Cell-cycle analysis of fission yeast cells by flow cytometry.

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