Frequency and coexistence of the C677T and A1298C polymorphisms of the MTHFR gene in Aures region of Algeria

Hayat D, Mouloud Y, Abdelali B. 2018. Frequency and coexistence of the C677T and A1298C polymorphisms of the MTHFR gene in Aures region of Algeria. Biodiversitas 19: 1169-1175. The aim of this study was to assess the frequency of the two most common polymorphisms C677T and A 1298C of the methylenete...

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Autores principales:	DJAARA HAYAT, YAHIA MOULOUD, BOUSSIF ABDELALI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2018
Materias:	5,10-methylenetetrahydrofolate reductase gene c677t a1298c snps coexisting genotypes real-time pcr-fret Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/c04019a2528140d88c07450580930c04
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spelling	oai:doaj.org-article:c04019a2528140d88c07450580930c042021-11-16T14:03:00ZFrequency and coexistence of the C677T and A1298C polymorphisms of the MTHFR gene in Aures region of Algeria1412-033X2085-472210.13057/biodiv/d190353https://doaj.org/article/c04019a2528140d88c07450580930c042018-05-01T00:00:00Zhttps://smujo.id/biodiv/article/view/2762https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Hayat D, Mouloud Y, Abdelali B. 2018. Frequency and coexistence of the C677T and A1298C polymorphisms of the MTHFR gene in Aures region of Algeria. Biodiversitas 19: 1169-1175. The aim of this study was to assess the frequency of the two most common polymorphisms C677T and A 1298C of the methylenetetrahydrofolate reductase (MTHFR) gene, as well as the coexistence of both these genetic variants in healthy subjects from part of the Algerian population (Aures Region). A total of 94 apparently healthy subjects were enrolled in the study group. The frequency of the both investigated genotypes of the both MTHFR gene polymorphisms (C677T and A1298C) was determined by using the Real-Time Polymerase Chain Reaction-Fluorescence Resonance Energy Transfer (Real-Time PCR-FRET) technic. The frequencies of C and T alleles of C677T polymorphism were 127 (67.55%), 61 (32.45%), and for CC, CT, and TT genotypes were 44 (46.48%), 39 (41.8%) and 11 (11.70%) respectively. Regarding the frequencies at position 1298, for A and C alleles were 147 (78.19%), 41 (21.81%), and for AA, AC, and CC genotypes were 60 (63.82%), 27 (28.72%) and 7 (7.44%) respectively. Also, our results indicated that no significant differences in the percentage distributions of the C677T (P=0.518) and A1298C (P=0.514) polymorphisms between males and females carriers. As noted in the findings, the most frequent coexistence of genotypes were 677CT/1298AA (29.78%), 677CC/1298AA (22.34%) and 677CC/1298AC (17.02%%). The coexistence of 677TT/1298AA (11.70%), 677CT/1298 AC (11.70%) and 677CC/1298 CC (7.44%) genotypes was observed less frequently and for 677TT/1298AC, 677CT/1298CC, 677TT/1298CC genotypes, it has been no observed in the studied population. The frequency of MTHFR 677 C and T alleles were 0.66 and 0.31, whereas that of MTHFR1298 A and C alleles were 0.77 and 0.21, respectively. The allelic distributions of the C677T polymorphism remain intermediate in the Aures region (Northeast of Algeria); that support the idea of a north-south gradient. For the A1298C SNP, our funding appears to be lower compared across populations. In addition, the frequency and coexistence of genotypes of the C677T and A 1298C MTHFR gene polymorphisms in the region studied are similar to other ethnic group populations.DJAARA HAYATYAHIA MOULOUDBOUSSIF ABDELALIMBI & UNS Soloarticle5,10-methylenetetrahydrofolate reductase genec677ta1298c snpscoexisting genotypesreal-time pcr-fretBiology (General)QH301-705.5ENBiodiversitas, Vol 19, Iss 3, Pp 1169-1175 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	5,10-methylenetetrahydrofolate reductase gene c677t a1298c snps coexisting genotypes real-time pcr-fret Biology (General) QH301-705.5
spellingShingle	5,10-methylenetetrahydrofolate reductase gene c677t a1298c snps coexisting genotypes real-time pcr-fret Biology (General) QH301-705.5 DJAARA HAYAT YAHIA MOULOUD BOUSSIF ABDELALI Frequency and coexistence of the C677T and A1298C polymorphisms of the MTHFR gene in Aures region of Algeria
description	Hayat D, Mouloud Y, Abdelali B. 2018. Frequency and coexistence of the C677T and A1298C polymorphisms of the MTHFR gene in Aures region of Algeria. Biodiversitas 19: 1169-1175. The aim of this study was to assess the frequency of the two most common polymorphisms C677T and A 1298C of the methylenetetrahydrofolate reductase (MTHFR) gene, as well as the coexistence of both these genetic variants in healthy subjects from part of the Algerian population (Aures Region). A total of 94 apparently healthy subjects were enrolled in the study group. The frequency of the both investigated genotypes of the both MTHFR gene polymorphisms (C677T and A1298C) was determined by using the Real-Time Polymerase Chain Reaction-Fluorescence Resonance Energy Transfer (Real-Time PCR-FRET) technic. The frequencies of C and T alleles of C677T polymorphism were 127 (67.55%), 61 (32.45%), and for CC, CT, and TT genotypes were 44 (46.48%), 39 (41.8%) and 11 (11.70%) respectively. Regarding the frequencies at position 1298, for A and C alleles were 147 (78.19%), 41 (21.81%), and for AA, AC, and CC genotypes were 60 (63.82%), 27 (28.72%) and 7 (7.44%) respectively. Also, our results indicated that no significant differences in the percentage distributions of the C677T (P=0.518) and A1298C (P=0.514) polymorphisms between males and females carriers. As noted in the findings, the most frequent coexistence of genotypes were 677CT/1298AA (29.78%), 677CC/1298AA (22.34%) and 677CC/1298AC (17.02%%). The coexistence of 677TT/1298AA (11.70%), 677CT/1298 AC (11.70%) and 677CC/1298 CC (7.44%) genotypes was observed less frequently and for 677TT/1298AC, 677CT/1298CC, 677TT/1298CC genotypes, it has been no observed in the studied population. The frequency of MTHFR 677 C and T alleles were 0.66 and 0.31, whereas that of MTHFR1298 A and C alleles were 0.77 and 0.21, respectively. The allelic distributions of the C677T polymorphism remain intermediate in the Aures region (Northeast of Algeria); that support the idea of a north-south gradient. For the A1298C SNP, our funding appears to be lower compared across populations. In addition, the frequency and coexistence of genotypes of the C677T and A 1298C MTHFR gene polymorphisms in the region studied are similar to other ethnic group populations.
format	article
author	DJAARA HAYAT YAHIA MOULOUD BOUSSIF ABDELALI
author_facet	DJAARA HAYAT YAHIA MOULOUD BOUSSIF ABDELALI
author_sort	DJAARA HAYAT
title	Frequency and coexistence of the C677T and A1298C polymorphisms of the MTHFR gene in Aures region of Algeria
title_short	Frequency and coexistence of the C677T and A1298C polymorphisms of the MTHFR gene in Aures region of Algeria
title_full	Frequency and coexistence of the C677T and A1298C polymorphisms of the MTHFR gene in Aures region of Algeria
title_fullStr	Frequency and coexistence of the C677T and A1298C polymorphisms of the MTHFR gene in Aures region of Algeria
title_full_unstemmed	Frequency and coexistence of the C677T and A1298C polymorphisms of the MTHFR gene in Aures region of Algeria
title_sort	frequency and coexistence of the c677t and a1298c polymorphisms of the mthfr gene in aures region of algeria
publisher	MBI & UNS Solo
publishDate	2018
url	https://doaj.org/article/c04019a2528140d88c07450580930c04
work_keys_str_mv	AT djaarahayat frequencyandcoexistenceofthec677tanda1298cpolymorphismsofthemthfrgeneinauresregionofalgeria AT yahiamouloud frequencyandcoexistenceofthec677tanda1298cpolymorphismsofthemthfrgeneinauresregionofalgeria AT boussifabdelali frequencyandcoexistenceofthec677tanda1298cpolymorphismsofthemthfrgeneinauresregionofalgeria
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Frequency and coexistence of the C677T and A1298C polymorphisms of the MTHFR gene in Aures region of Algeria

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