Impairment of the immune response after transcuticular introduction of the insect gonadoinhibitory and hemocytotoxic peptide Neb-colloostatin: A nanotech approach for pest control

Abstract This article shows that nanodiamonds can transmigrate through the insect cuticle easily, and the doses used were not hemocytotoxic and did not cause inhibition of cellular and humoral immune responses in larvae, pupae and adults of Tenebrio molitor. The examination of the nanodiamond biodis...

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Autores principales:	Elżbieta Czarniewska, Patryk Nowicki, Mariola Kuczer, Grzegorz Schroeder
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c046cb72d48d4b008652e628564029e1
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spelling	oai:doaj.org-article:c046cb72d48d4b008652e628564029e12021-12-02T15:10:01ZImpairment of the immune response after transcuticular introduction of the insect gonadoinhibitory and hemocytotoxic peptide Neb-colloostatin: A nanotech approach for pest control10.1038/s41598-019-46720-92045-2322https://doaj.org/article/c046cb72d48d4b008652e628564029e12019-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-46720-9https://doaj.org/toc/2045-2322Abstract This article shows that nanodiamonds can transmigrate through the insect cuticle easily, and the doses used were not hemocytotoxic and did not cause inhibition of cellular and humoral immune responses in larvae, pupae and adults of Tenebrio molitor. The examination of the nanodiamond biodistribution in insect cells demonstrated the presence of nanodiamond aggregates mainly in hemocytes, where nanoparticles were efficiently collected as a result of phagocytosis. To a lesser extent, nanodiamond aggregates were also detected in fat body cells, while they were not observed in Malpighian tubule cells. We functionalized nanodiamonds with Neb-colloostatin, an insect hemocytotoxic and gonadoinhibitory peptide, and we showed that this conjugate passed through the insect cuticle into the hemolymph, where the peptide complexed with the nanodiamonds induced apoptosis of hemocytes, significantly decreased the number of hemocytes circulating in the hemolymph and inhibited cellular and humoral immune responses in all developmental stages of insects. The results indicate that it is possible to introduce a peptide that interferes with the immunity and reproduction of insects to the interior of the insect body by means of a nanocarrier. In the future, the results of these studies may contribute to the development of new pest control agents.Elżbieta CzarniewskaPatryk NowickiMariola KuczerGrzegorz SchroederNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-12 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Elżbieta Czarniewska Patryk Nowicki Mariola Kuczer Grzegorz Schroeder Impairment of the immune response after transcuticular introduction of the insect gonadoinhibitory and hemocytotoxic peptide Neb-colloostatin: A nanotech approach for pest control
description	Abstract This article shows that nanodiamonds can transmigrate through the insect cuticle easily, and the doses used were not hemocytotoxic and did not cause inhibition of cellular and humoral immune responses in larvae, pupae and adults of Tenebrio molitor. The examination of the nanodiamond biodistribution in insect cells demonstrated the presence of nanodiamond aggregates mainly in hemocytes, where nanoparticles were efficiently collected as a result of phagocytosis. To a lesser extent, nanodiamond aggregates were also detected in fat body cells, while they were not observed in Malpighian tubule cells. We functionalized nanodiamonds with Neb-colloostatin, an insect hemocytotoxic and gonadoinhibitory peptide, and we showed that this conjugate passed through the insect cuticle into the hemolymph, where the peptide complexed with the nanodiamonds induced apoptosis of hemocytes, significantly decreased the number of hemocytes circulating in the hemolymph and inhibited cellular and humoral immune responses in all developmental stages of insects. The results indicate that it is possible to introduce a peptide that interferes with the immunity and reproduction of insects to the interior of the insect body by means of a nanocarrier. In the future, the results of these studies may contribute to the development of new pest control agents.
format	article
author	Elżbieta Czarniewska Patryk Nowicki Mariola Kuczer Grzegorz Schroeder
author_facet	Elżbieta Czarniewska Patryk Nowicki Mariola Kuczer Grzegorz Schroeder
author_sort	Elżbieta Czarniewska
title	Impairment of the immune response after transcuticular introduction of the insect gonadoinhibitory and hemocytotoxic peptide Neb-colloostatin: A nanotech approach for pest control
title_short	Impairment of the immune response after transcuticular introduction of the insect gonadoinhibitory and hemocytotoxic peptide Neb-colloostatin: A nanotech approach for pest control
title_full	Impairment of the immune response after transcuticular introduction of the insect gonadoinhibitory and hemocytotoxic peptide Neb-colloostatin: A nanotech approach for pest control
title_fullStr	Impairment of the immune response after transcuticular introduction of the insect gonadoinhibitory and hemocytotoxic peptide Neb-colloostatin: A nanotech approach for pest control
title_full_unstemmed	Impairment of the immune response after transcuticular introduction of the insect gonadoinhibitory and hemocytotoxic peptide Neb-colloostatin: A nanotech approach for pest control
title_sort	impairment of the immune response after transcuticular introduction of the insect gonadoinhibitory and hemocytotoxic peptide neb-colloostatin: a nanotech approach for pest control
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/c046cb72d48d4b008652e628564029e1
work_keys_str_mv	AT elzbietaczarniewska impairmentoftheimmuneresponseaftertranscuticularintroductionoftheinsectgonadoinhibitoryandhemocytotoxicpeptidenebcolloostatinananotechapproachforpestcontrol AT patryknowicki impairmentoftheimmuneresponseaftertranscuticularintroductionoftheinsectgonadoinhibitoryandhemocytotoxicpeptidenebcolloostatinananotechapproachforpestcontrol AT mariolakuczer impairmentoftheimmuneresponseaftertranscuticularintroductionoftheinsectgonadoinhibitoryandhemocytotoxicpeptidenebcolloostatinananotechapproachforpestcontrol AT grzegorzschroeder impairmentoftheimmuneresponseaftertranscuticularintroductionoftheinsectgonadoinhibitoryandhemocytotoxicpeptidenebcolloostatinananotechapproachforpestcontrol
_version_	1718387776809861120

Impairment of the immune response after transcuticular introduction of the insect gonadoinhibitory and hemocytotoxic peptide Neb-colloostatin: A nanotech approach for pest control

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