Minimal system for assembly of SARS-CoV-2 virus like particles

Abstract SARS-CoV-2 virus is the causative agent of COVID-19. Here we demonstrate that non-infectious SARS-CoV-2 virus like particles (VLPs) can be assembled by co-expressing the viral proteins S, M and E in mammalian cells. The assembled SARS-CoV-2 VLPs possess S protein spikes on particle exterior...

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Autores principales:	Heather Swann, Abhimanyu Sharma, Benjamin Preece, Abby Peterson, Crystal Eldridge, David M. Belnap, Michael Vershinin, Saveez Saffarian
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c04addeea9b342c79dfabdf0aada9795
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spelling	oai:doaj.org-article:c04addeea9b342c79dfabdf0aada97952021-12-02T12:40:40ZMinimal system for assembly of SARS-CoV-2 virus like particles10.1038/s41598-020-78656-w2045-2322https://doaj.org/article/c04addeea9b342c79dfabdf0aada97952020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78656-whttps://doaj.org/toc/2045-2322Abstract SARS-CoV-2 virus is the causative agent of COVID-19. Here we demonstrate that non-infectious SARS-CoV-2 virus like particles (VLPs) can be assembled by co-expressing the viral proteins S, M and E in mammalian cells. The assembled SARS-CoV-2 VLPs possess S protein spikes on particle exterior, making them ideal for vaccine development. The particles range in shape from spherical to elongated with a characteristic size of 129 ± 32 nm. We further show that SARS-CoV-2 VLPs dried in ambient conditions can retain their structural integrity upon repeated scans with Atomic Force Microscopy up to a peak force of 1 nN.Heather SwannAbhimanyu SharmaBenjamin PreeceAbby PetersonCrystal EldridgeDavid M. BelnapMichael VershininSaveez SaffarianNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-5 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Heather Swann Abhimanyu Sharma Benjamin Preece Abby Peterson Crystal Eldridge David M. Belnap Michael Vershinin Saveez Saffarian Minimal system for assembly of SARS-CoV-2 virus like particles
description	Abstract SARS-CoV-2 virus is the causative agent of COVID-19. Here we demonstrate that non-infectious SARS-CoV-2 virus like particles (VLPs) can be assembled by co-expressing the viral proteins S, M and E in mammalian cells. The assembled SARS-CoV-2 VLPs possess S protein spikes on particle exterior, making them ideal for vaccine development. The particles range in shape from spherical to elongated with a characteristic size of 129 ± 32 nm. We further show that SARS-CoV-2 VLPs dried in ambient conditions can retain their structural integrity upon repeated scans with Atomic Force Microscopy up to a peak force of 1 nN.
format	article
author	Heather Swann Abhimanyu Sharma Benjamin Preece Abby Peterson Crystal Eldridge David M. Belnap Michael Vershinin Saveez Saffarian
author_facet	Heather Swann Abhimanyu Sharma Benjamin Preece Abby Peterson Crystal Eldridge David M. Belnap Michael Vershinin Saveez Saffarian
author_sort	Heather Swann
title	Minimal system for assembly of SARS-CoV-2 virus like particles
title_short	Minimal system for assembly of SARS-CoV-2 virus like particles
title_full	Minimal system for assembly of SARS-CoV-2 virus like particles
title_fullStr	Minimal system for assembly of SARS-CoV-2 virus like particles
title_full_unstemmed	Minimal system for assembly of SARS-CoV-2 virus like particles
title_sort	minimal system for assembly of sars-cov-2 virus like particles
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/c04addeea9b342c79dfabdf0aada9795
work_keys_str_mv	AT heatherswann minimalsystemforassemblyofsarscov2viruslikeparticles AT abhimanyusharma minimalsystemforassemblyofsarscov2viruslikeparticles AT benjaminpreece minimalsystemforassemblyofsarscov2viruslikeparticles AT abbypeterson minimalsystemforassemblyofsarscov2viruslikeparticles AT crystaleldridge minimalsystemforassemblyofsarscov2viruslikeparticles AT davidmbelnap minimalsystemforassemblyofsarscov2viruslikeparticles AT michaelvershinin minimalsystemforassemblyofsarscov2viruslikeparticles AT saveezsaffarian minimalsystemforassemblyofsarscov2viruslikeparticles
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Minimal system for assembly of SARS-CoV-2 virus like particles

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