Size and specimen-dependent strategy for x-ray micro-ct and tem correlative analysis of nervous system samples

Abstract Correlative approaches are a powerful tool in the investigation of biological samples, but require specific preparation procedures to maintain the strength of the employed methods. Here we report the optimization of the embedding protocol of nervous system samples for a correlative synchrot...

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Autores principales:	P. Parlanti, V. Cappello, F. Brun, G. Tromba, R. Rigolio, I. Tonazzini, M. Cecchini, V. Piazza, M. Gemmi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c04b3a9367d34ed2ad79ebafb1a853ac
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spelling	oai:doaj.org-article:c04b3a9367d34ed2ad79ebafb1a853ac2021-12-02T15:05:58ZSize and specimen-dependent strategy for x-ray micro-ct and tem correlative analysis of nervous system samples10.1038/s41598-017-02998-12045-2322https://doaj.org/article/c04b3a9367d34ed2ad79ebafb1a853ac2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02998-1https://doaj.org/toc/2045-2322Abstract Correlative approaches are a powerful tool in the investigation of biological samples, but require specific preparation procedures to maintain the strength of the employed methods. Here we report the optimization of the embedding protocol of nervous system samples for a correlative synchrotron X-ray computed microtomography (micro-CT) and transmission electron microscopy (TEM) approach. We demonstrate that it is possible to locate, with the micrometric resolution of micro-CT, specific volumes of interest for a further ultrastructural characterization to be performed with TEM. This approach can be applied to samples of different size and morphology up to several cm. Our optimized method represents an invaluable tool for investigating those pathologies in which microscopic alterations are localized in few confined regions, rather than diffused in entire tissues, organs or systems. We present a proof of concept of our method in a mouse model of Globoid Cells Leukodistrophy.P. ParlantiV. CappelloF. BrunG. TrombaR. RigolioI. TonazziniM. CecchiniV. PiazzaM. GemmiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q P. Parlanti V. Cappello F. Brun G. Tromba R. Rigolio I. Tonazzini M. Cecchini V. Piazza M. Gemmi Size and specimen-dependent strategy for x-ray micro-ct and tem correlative analysis of nervous system samples
description	Abstract Correlative approaches are a powerful tool in the investigation of biological samples, but require specific preparation procedures to maintain the strength of the employed methods. Here we report the optimization of the embedding protocol of nervous system samples for a correlative synchrotron X-ray computed microtomography (micro-CT) and transmission electron microscopy (TEM) approach. We demonstrate that it is possible to locate, with the micrometric resolution of micro-CT, specific volumes of interest for a further ultrastructural characterization to be performed with TEM. This approach can be applied to samples of different size and morphology up to several cm. Our optimized method represents an invaluable tool for investigating those pathologies in which microscopic alterations are localized in few confined regions, rather than diffused in entire tissues, organs or systems. We present a proof of concept of our method in a mouse model of Globoid Cells Leukodistrophy.
format	article
author	P. Parlanti V. Cappello F. Brun G. Tromba R. Rigolio I. Tonazzini M. Cecchini V. Piazza M. Gemmi
author_facet	P. Parlanti V. Cappello F. Brun G. Tromba R. Rigolio I. Tonazzini M. Cecchini V. Piazza M. Gemmi
author_sort	P. Parlanti
title	Size and specimen-dependent strategy for x-ray micro-ct and tem correlative analysis of nervous system samples
title_short	Size and specimen-dependent strategy for x-ray micro-ct and tem correlative analysis of nervous system samples
title_full	Size and specimen-dependent strategy for x-ray micro-ct and tem correlative analysis of nervous system samples
title_fullStr	Size and specimen-dependent strategy for x-ray micro-ct and tem correlative analysis of nervous system samples
title_full_unstemmed	Size and specimen-dependent strategy for x-ray micro-ct and tem correlative analysis of nervous system samples
title_sort	size and specimen-dependent strategy for x-ray micro-ct and tem correlative analysis of nervous system samples
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c04b3a9367d34ed2ad79ebafb1a853ac
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Size and specimen-dependent strategy for x-ray micro-ct and tem correlative analysis of nervous system samples

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