Antimicrobial quaternary ammonium organosilane cross-linked nanofibrous collagen scaffolds for tissue engineering

Chetna Dhand,1,2,* Yamini Balakrishnan,3,* Seow Theng Ong,4,* Neeraj Dwivedi,5 Jayarama R Venugopal,6 Sriram Harini,1 Chak Ming Leung,3 Kenny Zhi Wei Low,7 Xian Jun Loh,7 Roger W Beuerman,1,2 Seeram Ramakrishna,8 Navin Kumar Verma,1,4 Rajamani Lakshminarayanan1,2 1Anti-Infectives Research Group, Si...

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Autores principales:	Dhand C, Balakrishnan Y, Ong ST, Dwivedi N, Venugopal JR, Harini S, Leung CM, Low KZW, Loh XJ, Beuerman RW, Ramakrishna S, Verma NK, Lakshminarayanan R
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	quaternaryammonium organosilane collagen electrospinning crosslinking tissue engineering antimicrobial Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/c06aa56991d34a979c01075625829dc7
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spelling	oai:doaj.org-article:c06aa56991d34a979c01075625829dc72021-12-02T07:22:24ZAntimicrobial quaternary ammonium organosilane cross-linked nanofibrous collagen scaffolds for tissue engineering1178-2013https://doaj.org/article/c06aa56991d34a979c01075625829dc72018-08-01T00:00:00Zhttps://www.dovepress.com/antimicrobial-quaternary-ammonium-organosilane-cross-linked-nanofibrou-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Chetna Dhand,1,2,* Yamini Balakrishnan,3,* Seow Theng Ong,4,* Neeraj Dwivedi,5 Jayarama R Venugopal,6 Sriram Harini,1 Chak Ming Leung,3 Kenny Zhi Wei Low,7 Xian Jun Loh,7 Roger W Beuerman,1,2 Seeram Ramakrishna,8 Navin Kumar Verma,1,4 Rajamani Lakshminarayanan1,2 1Anti-Infectives Research Group, Singapore Eye Research Institute, The Academia, Discovery Tower, Singapore; 2Ophthalmology and Visual Sciences Academic Clinical Program, Duke-NUS Graduate Medical School, Singapore; 3Department of Bioengineering, National University of Singapore, Singapore; 4Dermatology and Skin Biology, Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore; 5Department of Electrical and Computer Engineering, National University of Singapore, Singapore; 6Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang, Gambang, Malaysia; 7Department of Mechanical Engineering, Faculty of Engineering, Center for Nanofibers and Nanotechnology, National University of Singapore, Singapore; 8Soft Materials Department, Institute of Materials Research and Engineering, ASTAR (Agency for Science, Technology and Research, Singapore These authors contributed equally to this work Introduction: In search for cross-linkers with multifunctional characteristics, the present work investigated the utility of quaternary ammonium organosilane (QOS) as a potential cross-linker for electrospun collagen nanofibers. We hypothesized that the quaternary ammonium ions improve the electrospinnability by reducing the surface tension and confer antimicrobial properties, while the formation of siloxane after alkaline hydrolysis could cross-link collagen and stimulate cell proliferation. Materials and methods: QOS collagen nanofibers were electrospun by incorporating various concentrations of QOS (0.1%–10% w/w) and were cross-linked in situ after exposure to ammonium carbonate. The QOS cross-linked scaffolds were characterized and their biological properties were evaluated in terms of their biocompatibility, cellular adhesion and metabolic activity for primary human dermal fibroblasts and human fetal osteoblasts. Results and discussion: The study revealed that 1) QOS cross-linking increased the flexibility of otherwise rigid collagen nanofibers and improved the thermal stability; 2) QOS cross-linked mats displayed potent antibacterial activity and 3) the biocompatibility of the composite mats depended on the amount of QOS present in dope solution – at low QOS concentrations (0.1% w/w), the mats promoted mammalian cell proliferation and growth, whereas at higher QOS concentrations, cytotoxic effect was observed. Conclusion: This study demonstrates that QOS cross-linked mats possess anti-infective properties and confer niches for cellular growth and proliferation, thus offering a useful approach, which is important for hard and soft tissue engineering and regenerative medicine. Keywords: anti-infective wound dressing, cyto-compatible nanofibre, electrospinning, cost-effective cross-linker, tissue regeneration, antimicrobialDhand CBalakrishnan YOng STDwivedi NVenugopal JRHarini SLeung CMLow KZWLoh XJBeuerman RWRamakrishna SVerma NKLakshminarayanan RDove Medical Pressarticlequaternaryammonium organosilanecollagenelectrospinningcrosslinkingtissue engineeringantimicrobialMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 4473-4492 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	quaternaryammonium organosilane collagen electrospinning crosslinking tissue engineering antimicrobial Medicine (General) R5-920
spellingShingle	quaternaryammonium organosilane collagen electrospinning crosslinking tissue engineering antimicrobial Medicine (General) R5-920 Dhand C Balakrishnan Y Ong ST Dwivedi N Venugopal JR Harini S Leung CM Low KZW Loh XJ Beuerman RW Ramakrishna S Verma NK Lakshminarayanan R Antimicrobial quaternary ammonium organosilane cross-linked nanofibrous collagen scaffolds for tissue engineering
description	Chetna Dhand,1,2,* Yamini Balakrishnan,3,* Seow Theng Ong,4,* Neeraj Dwivedi,5 Jayarama R Venugopal,6 Sriram Harini,1 Chak Ming Leung,3 Kenny Zhi Wei Low,7 Xian Jun Loh,7 Roger W Beuerman,1,2 Seeram Ramakrishna,8 Navin Kumar Verma,1,4 Rajamani Lakshminarayanan1,2 1Anti-Infectives Research Group, Singapore Eye Research Institute, The Academia, Discovery Tower, Singapore; 2Ophthalmology and Visual Sciences Academic Clinical Program, Duke-NUS Graduate Medical School, Singapore; 3Department of Bioengineering, National University of Singapore, Singapore; 4Dermatology and Skin Biology, Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore; 5Department of Electrical and Computer Engineering, National University of Singapore, Singapore; 6Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang, Gambang, Malaysia; 7Department of Mechanical Engineering, Faculty of Engineering, Center for Nanofibers and Nanotechnology, National University of Singapore, Singapore; 8Soft Materials Department, Institute of Materials Research and Engineering, ASTAR (Agency for Science, Technology and Research, Singapore These authors contributed equally to this work Introduction: In search for cross-linkers with multifunctional characteristics, the present work investigated the utility of quaternary ammonium organosilane (QOS) as a potential cross-linker for electrospun collagen nanofibers. We hypothesized that the quaternary ammonium ions improve the electrospinnability by reducing the surface tension and confer antimicrobial properties, while the formation of siloxane after alkaline hydrolysis could cross-link collagen and stimulate cell proliferation. Materials and methods: QOS collagen nanofibers were electrospun by incorporating various concentrations of QOS (0.1%–10% w/w) and were cross-linked in situ after exposure to ammonium carbonate. The QOS cross-linked scaffolds were characterized and their biological properties were evaluated in terms of their biocompatibility, cellular adhesion and metabolic activity for primary human dermal fibroblasts and human fetal osteoblasts. Results and discussion: The study revealed that 1) QOS cross-linking increased the flexibility of otherwise rigid collagen nanofibers and improved the thermal stability; 2) QOS cross-linked mats displayed potent antibacterial activity and 3) the biocompatibility of the composite mats depended on the amount of QOS present in dope solution – at low QOS concentrations (0.1% w/w), the mats promoted mammalian cell proliferation and growth, whereas at higher QOS concentrations, cytotoxic effect was observed. Conclusion: This study demonstrates that QOS cross-linked mats possess anti-infective properties and confer niches for cellular growth and proliferation, thus offering a useful approach, which is important for hard and soft tissue engineering and regenerative medicine. Keywords: anti-infective wound dressing, cyto-compatible nanofibre, electrospinning, cost-effective cross-linker, tissue regeneration, antimicrobial
format	article
author	Dhand C Balakrishnan Y Ong ST Dwivedi N Venugopal JR Harini S Leung CM Low KZW Loh XJ Beuerman RW Ramakrishna S Verma NK Lakshminarayanan R
author_facet	Dhand C Balakrishnan Y Ong ST Dwivedi N Venugopal JR Harini S Leung CM Low KZW Loh XJ Beuerman RW Ramakrishna S Verma NK Lakshminarayanan R
author_sort	Dhand C
title	Antimicrobial quaternary ammonium organosilane cross-linked nanofibrous collagen scaffolds for tissue engineering
title_short	Antimicrobial quaternary ammonium organosilane cross-linked nanofibrous collagen scaffolds for tissue engineering
title_full	Antimicrobial quaternary ammonium organosilane cross-linked nanofibrous collagen scaffolds for tissue engineering
title_fullStr	Antimicrobial quaternary ammonium organosilane cross-linked nanofibrous collagen scaffolds for tissue engineering
title_full_unstemmed	Antimicrobial quaternary ammonium organosilane cross-linked nanofibrous collagen scaffolds for tissue engineering
title_sort	antimicrobial quaternary ammonium organosilane cross-linked nanofibrous collagen scaffolds for tissue engineering
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/c06aa56991d34a979c01075625829dc7
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Antimicrobial quaternary ammonium organosilane cross-linked nanofibrous collagen scaffolds for tissue engineering

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