NuA4 and SAGA acetyltransferase complexes cooperate for repair of DNA breaks by homologous recombination.

Chromatin modifying complexes play important yet not fully defined roles in DNA repair processes. The essential NuA4 histone acetyltransferase (HAT) complex is recruited to double-strand break (DSB) sites and spreads along with DNA end resection. As predicted, NuA4 acetylates surrounding nucleosomes...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xue Cheng, Valérie Côté, Jacques Côté
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/c07c9a3c9fda49bf89c41828cf83325a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c07c9a3c9fda49bf89c41828cf83325a
record_format	dspace
spelling	oai:doaj.org-article:c07c9a3c9fda49bf89c41828cf83325a2021-12-02T20:02:47ZNuA4 and SAGA acetyltransferase complexes cooperate for repair of DNA breaks by homologous recombination.1553-73901553-740410.1371/journal.pgen.1009459https://doaj.org/article/c07c9a3c9fda49bf89c41828cf83325a2021-07-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009459https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404Chromatin modifying complexes play important yet not fully defined roles in DNA repair processes. The essential NuA4 histone acetyltransferase (HAT) complex is recruited to double-strand break (DSB) sites and spreads along with DNA end resection. As predicted, NuA4 acetylates surrounding nucleosomes upon DSB induction and defects in its activity correlate with altered DNA end resection and Rad51 recombinase recruitment. Importantly, we show that NuA4 is also recruited to the donor sequence during recombination along with increased H4 acetylation, indicating a direct role during strand invasion/D-loop formation after resection. We found that NuA4 cooperates locally with another HAT, the SAGA complex, during DSB repair as their combined action is essential for DNA end resection to occur. This cooperation of NuA4 and SAGA is required for recruitment of ATP-dependent chromatin remodelers, targeted acetylation of repair factors and homologous recombination. Our work reveals a multifaceted and conserved cooperation mechanism between acetyltransferase complexes to allow repair of DNA breaks by homologous recombination.Xue ChengValérie CôtéJacques CôtéPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 7, p e1009459 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Xue Cheng Valérie Côté Jacques Côté NuA4 and SAGA acetyltransferase complexes cooperate for repair of DNA breaks by homologous recombination.
description	Chromatin modifying complexes play important yet not fully defined roles in DNA repair processes. The essential NuA4 histone acetyltransferase (HAT) complex is recruited to double-strand break (DSB) sites and spreads along with DNA end resection. As predicted, NuA4 acetylates surrounding nucleosomes upon DSB induction and defects in its activity correlate with altered DNA end resection and Rad51 recombinase recruitment. Importantly, we show that NuA4 is also recruited to the donor sequence during recombination along with increased H4 acetylation, indicating a direct role during strand invasion/D-loop formation after resection. We found that NuA4 cooperates locally with another HAT, the SAGA complex, during DSB repair as their combined action is essential for DNA end resection to occur. This cooperation of NuA4 and SAGA is required for recruitment of ATP-dependent chromatin remodelers, targeted acetylation of repair factors and homologous recombination. Our work reveals a multifaceted and conserved cooperation mechanism between acetyltransferase complexes to allow repair of DNA breaks by homologous recombination.
format	article
author	Xue Cheng Valérie Côté Jacques Côté
author_facet	Xue Cheng Valérie Côté Jacques Côté
author_sort	Xue Cheng
title	NuA4 and SAGA acetyltransferase complexes cooperate for repair of DNA breaks by homologous recombination.
title_short	NuA4 and SAGA acetyltransferase complexes cooperate for repair of DNA breaks by homologous recombination.
title_full	NuA4 and SAGA acetyltransferase complexes cooperate for repair of DNA breaks by homologous recombination.
title_fullStr	NuA4 and SAGA acetyltransferase complexes cooperate for repair of DNA breaks by homologous recombination.
title_full_unstemmed	NuA4 and SAGA acetyltransferase complexes cooperate for repair of DNA breaks by homologous recombination.
title_sort	nua4 and saga acetyltransferase complexes cooperate for repair of dna breaks by homologous recombination.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/c07c9a3c9fda49bf89c41828cf83325a
work_keys_str_mv	AT xuecheng nua4andsagaacetyltransferasecomplexescooperateforrepairofdnabreaksbyhomologousrecombination AT valeriecote nua4andsagaacetyltransferasecomplexescooperateforrepairofdnabreaksbyhomologousrecombination AT jacquescote nua4andsagaacetyltransferasecomplexescooperateforrepairofdnabreaksbyhomologousrecombination
_version_	1718375684219338752

NuA4 and SAGA acetyltransferase complexes cooperate for repair of DNA breaks by homologous recombination.

Ejemplares similares