A fluorescent, genetically-encoded voltage probe capable of resolving action potentials.

There is a pressing need in neuroscience for genetically-encoded, fluorescent voltage probes that can be targeted to specific neurons and circuits to allow study of neural activity using fluorescent imaging. We created 90 constructs in which the voltage sensing portion (S1-S4) of Ciona intestinalis...

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Autores principales:	Lauren Barnett, Jelena Platisa, Marko Popovic, Vincent A Pieribone, Thomas Hughes
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c07ce5a741a8400f87fdcd2586fc925f
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spelling	oai:doaj.org-article:c07ce5a741a8400f87fdcd2586fc925f2021-11-18T07:06:28ZA fluorescent, genetically-encoded voltage probe capable of resolving action potentials.1932-620310.1371/journal.pone.0043454https://doaj.org/article/c07ce5a741a8400f87fdcd2586fc925f2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22970127/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203There is a pressing need in neuroscience for genetically-encoded, fluorescent voltage probes that can be targeted to specific neurons and circuits to allow study of neural activity using fluorescent imaging. We created 90 constructs in which the voltage sensing portion (S1-S4) of Ciona intestinalis voltage sensitive phosphatase (CiVSP) was fused to circularly permuted eGFP. This led to ElectricPk, a probe that is an order of magnitude faster (taus ~1-2 ms) than any currently published fluorescent protein-based voltage probe. ElectricPk can follow the rise and fall of neuronal action potentials with a modest decrease in fluorescence intensity (~0.7% ΔF/F). The probe has a nearly linear fluorescence/membrane potential response to both hyperpolarizing and depolarizing steps. This is the first probe based on CiVSP that captures the rapid movements of the voltage sensor, suggesting that voltage probes designed with circularly permuted fluorescent proteins may have some advantages.Lauren BarnettJelena PlatisaMarko PopovicVincent A PieriboneThomas HughesPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 9, p e43454 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lauren Barnett Jelena Platisa Marko Popovic Vincent A Pieribone Thomas Hughes A fluorescent, genetically-encoded voltage probe capable of resolving action potentials.
description	There is a pressing need in neuroscience for genetically-encoded, fluorescent voltage probes that can be targeted to specific neurons and circuits to allow study of neural activity using fluorescent imaging. We created 90 constructs in which the voltage sensing portion (S1-S4) of Ciona intestinalis voltage sensitive phosphatase (CiVSP) was fused to circularly permuted eGFP. This led to ElectricPk, a probe that is an order of magnitude faster (taus ~1-2 ms) than any currently published fluorescent protein-based voltage probe. ElectricPk can follow the rise and fall of neuronal action potentials with a modest decrease in fluorescence intensity (~0.7% ΔF/F). The probe has a nearly linear fluorescence/membrane potential response to both hyperpolarizing and depolarizing steps. This is the first probe based on CiVSP that captures the rapid movements of the voltage sensor, suggesting that voltage probes designed with circularly permuted fluorescent proteins may have some advantages.
format	article
author	Lauren Barnett Jelena Platisa Marko Popovic Vincent A Pieribone Thomas Hughes
author_facet	Lauren Barnett Jelena Platisa Marko Popovic Vincent A Pieribone Thomas Hughes
author_sort	Lauren Barnett
title	A fluorescent, genetically-encoded voltage probe capable of resolving action potentials.
title_short	A fluorescent, genetically-encoded voltage probe capable of resolving action potentials.
title_full	A fluorescent, genetically-encoded voltage probe capable of resolving action potentials.
title_fullStr	A fluorescent, genetically-encoded voltage probe capable of resolving action potentials.
title_full_unstemmed	A fluorescent, genetically-encoded voltage probe capable of resolving action potentials.
title_sort	fluorescent, genetically-encoded voltage probe capable of resolving action potentials.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/c07ce5a741a8400f87fdcd2586fc925f
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A fluorescent, genetically-encoded voltage probe capable of resolving action potentials.

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