Allorecognition triggers autophagy and subsequent necrosis in the cnidarian Hydractinia symbiolongicarpus.

Transitory fusion is an allorecognition phenotype displayed by the colonial hydroid Hydractinia symbiolongicarpus when interacting colonies share some, but not all, loci within the allorecognition gene complex (ARC). The phenotype is characterized by an initial fusion followed by subsequent cell dea...

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Auteurs principaux:	Leo W Buss, Christopher Anderson, Erica Westerman, Chad Kritzberger, Monita Poudyal, Maria A Moreno, Fadi G Lakkis
Format:	article
Langue:	EN
Publié:	Public Library of Science (PLoS) 2012
Sujets:	Medicine R Science Q
Accès en ligne:	https://doaj.org/article/c0a317a043604adc920c40d0bc178d2a
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spelling	oai:doaj.org-article:c0a317a043604adc920c40d0bc178d2a2021-11-18T08:09:24ZAllorecognition triggers autophagy and subsequent necrosis in the cnidarian Hydractinia symbiolongicarpus.1932-620310.1371/journal.pone.0048914https://doaj.org/article/c0a317a043604adc920c40d0bc178d2a2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23145018/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Transitory fusion is an allorecognition phenotype displayed by the colonial hydroid Hydractinia symbiolongicarpus when interacting colonies share some, but not all, loci within the allorecognition gene complex (ARC). The phenotype is characterized by an initial fusion followed by subsequent cell death resulting in separation of the two incompatible colonies. We here characterize this cell death process using scanning electron microscopy (SEM), transmission electron microscopy (TEM), and continuous in vivo digital microscopy. These techniques reveal widespread autophagy and subsequent necrosis in both colony and grafted polyp assays. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays and ultrastructural observations revealed no evidence of apoptosis. Pharmacological inhibition of autophagy using 3-methyladenine (3-MA) completely suppressed transitory fusion in vivo in colony assays. Rapamycin did not have a significant effect in the same assays. These results establish the hydroid allorecognition system as a novel model for the study of cell death.Leo W BussChristopher AndersonErica WestermanChad KritzbergerMonita PoudyalMaria A MorenoFadi G LakkisPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 11, p e48914 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Leo W Buss Christopher Anderson Erica Westerman Chad Kritzberger Monita Poudyal Maria A Moreno Fadi G Lakkis Allorecognition triggers autophagy and subsequent necrosis in the cnidarian Hydractinia symbiolongicarpus.
description	Transitory fusion is an allorecognition phenotype displayed by the colonial hydroid Hydractinia symbiolongicarpus when interacting colonies share some, but not all, loci within the allorecognition gene complex (ARC). The phenotype is characterized by an initial fusion followed by subsequent cell death resulting in separation of the two incompatible colonies. We here characterize this cell death process using scanning electron microscopy (SEM), transmission electron microscopy (TEM), and continuous in vivo digital microscopy. These techniques reveal widespread autophagy and subsequent necrosis in both colony and grafted polyp assays. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays and ultrastructural observations revealed no evidence of apoptosis. Pharmacological inhibition of autophagy using 3-methyladenine (3-MA) completely suppressed transitory fusion in vivo in colony assays. Rapamycin did not have a significant effect in the same assays. These results establish the hydroid allorecognition system as a novel model for the study of cell death.
format	article
author	Leo W Buss Christopher Anderson Erica Westerman Chad Kritzberger Monita Poudyal Maria A Moreno Fadi G Lakkis
author_facet	Leo W Buss Christopher Anderson Erica Westerman Chad Kritzberger Monita Poudyal Maria A Moreno Fadi G Lakkis
author_sort	Leo W Buss
title	Allorecognition triggers autophagy and subsequent necrosis in the cnidarian Hydractinia symbiolongicarpus.
title_short	Allorecognition triggers autophagy and subsequent necrosis in the cnidarian Hydractinia symbiolongicarpus.
title_full	Allorecognition triggers autophagy and subsequent necrosis in the cnidarian Hydractinia symbiolongicarpus.
title_fullStr	Allorecognition triggers autophagy and subsequent necrosis in the cnidarian Hydractinia symbiolongicarpus.
title_full_unstemmed	Allorecognition triggers autophagy and subsequent necrosis in the cnidarian Hydractinia symbiolongicarpus.
title_sort	allorecognition triggers autophagy and subsequent necrosis in the cnidarian hydractinia symbiolongicarpus.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/c0a317a043604adc920c40d0bc178d2a
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Allorecognition triggers autophagy and subsequent necrosis in the cnidarian Hydractinia symbiolongicarpus.

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