Thiol Compounds, Pre-Conditioning and Orientation of Explants – Important Factors Affecting Regeneration from Cotyledons of Legume Crop Sesbania Aculeata

Sesbania aculeata is a multipurpose legume crop grown primarily for green manuring in the rice-based cropping system. Besides this, it is an industrial crop and is also used as food in many parts of the world. The present work reports for optimization of various parameters (growth medium, plant grow...

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Autores principales:	Mehta Nikhil, Rao Priyanka, Saini Raman
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2021
Materias:	cotyledon explant’s orientation organogenesis pre-conditioning regeneration shoot multiplication Agriculture S
Acceso en línea:	https://doaj.org/article/c0abb7c35aaf4808ba778f6b7d0271dc
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spelling	oai:doaj.org-article:c0abb7c35aaf4808ba778f6b7d0271dc2021-12-05T14:11:05ZThiol Compounds, Pre-Conditioning and Orientation of Explants – Important Factors Affecting Regeneration from Cotyledons of Legume Crop Sesbania Aculeata1338-437610.2478/agri-2021-0009https://doaj.org/article/c0abb7c35aaf4808ba778f6b7d0271dc2021-10-01T00:00:00Zhttps://doi.org/10.2478/agri-2021-0009https://doaj.org/toc/1338-4376Sesbania aculeata is a multipurpose legume crop grown primarily for green manuring in the rice-based cropping system. Besides this, it is an industrial crop and is also used as food in many parts of the world. The present work reports for optimization of various parameters (growth medium, plant growth regulators, pre-conditioning, orientation of explant, and presence of thiol compounds) affecting in vitro regeneration using mature cotyledon explants. The 5-day-old mature cotyledon explants excised from seedlings grown on Murashige and Skoog (MS) salts and Gamborg (B5) vitamins medium containing 15 μM 6-benzylaminopurine were cultured with its adaxial side facing on medium containing 2.5 μM 6-benzylaminopurine and 50 mg/L thiourea and produced multiple shoots (7 ‒ 8) in 100% cultures within 28 days. Healthy shoots were rooted on half-strength Murashige and Skoog (MS) salts and full-strength vitamins medium augmented with 2.5 μM indole-3-butyric acid.Mehta NikhilRao PriyankaSaini RamanSciendoarticlecotyledonexplant’s orientationorganogenesispre-conditioningregenerationshoot multiplicationAgricultureSENAgriculture, Vol 67, Iss 3, Pp 95-102 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cotyledon explant’s orientation organogenesis pre-conditioning regeneration shoot multiplication Agriculture S
spellingShingle	cotyledon explant’s orientation organogenesis pre-conditioning regeneration shoot multiplication Agriculture S Mehta Nikhil Rao Priyanka Saini Raman Thiol Compounds, Pre-Conditioning and Orientation of Explants – Important Factors Affecting Regeneration from Cotyledons of Legume Crop Sesbania Aculeata
description	Sesbania aculeata is a multipurpose legume crop grown primarily for green manuring in the rice-based cropping system. Besides this, it is an industrial crop and is also used as food in many parts of the world. The present work reports for optimization of various parameters (growth medium, plant growth regulators, pre-conditioning, orientation of explant, and presence of thiol compounds) affecting in vitro regeneration using mature cotyledon explants. The 5-day-old mature cotyledon explants excised from seedlings grown on Murashige and Skoog (MS) salts and Gamborg (B5) vitamins medium containing 15 μM 6-benzylaminopurine were cultured with its adaxial side facing on medium containing 2.5 μM 6-benzylaminopurine and 50 mg/L thiourea and produced multiple shoots (7 ‒ 8) in 100% cultures within 28 days. Healthy shoots were rooted on half-strength Murashige and Skoog (MS) salts and full-strength vitamins medium augmented with 2.5 μM indole-3-butyric acid.
format	article
author	Mehta Nikhil Rao Priyanka Saini Raman
author_facet	Mehta Nikhil Rao Priyanka Saini Raman
author_sort	Mehta Nikhil
title	Thiol Compounds, Pre-Conditioning and Orientation of Explants – Important Factors Affecting Regeneration from Cotyledons of Legume Crop Sesbania Aculeata
title_short	Thiol Compounds, Pre-Conditioning and Orientation of Explants – Important Factors Affecting Regeneration from Cotyledons of Legume Crop Sesbania Aculeata
title_full	Thiol Compounds, Pre-Conditioning and Orientation of Explants – Important Factors Affecting Regeneration from Cotyledons of Legume Crop Sesbania Aculeata
title_fullStr	Thiol Compounds, Pre-Conditioning and Orientation of Explants – Important Factors Affecting Regeneration from Cotyledons of Legume Crop Sesbania Aculeata
title_full_unstemmed	Thiol Compounds, Pre-Conditioning and Orientation of Explants – Important Factors Affecting Regeneration from Cotyledons of Legume Crop Sesbania Aculeata
title_sort	thiol compounds, pre-conditioning and orientation of explants – important factors affecting regeneration from cotyledons of legume crop sesbania aculeata
publisher	Sciendo
publishDate	2021
url	https://doaj.org/article/c0abb7c35aaf4808ba778f6b7d0271dc
work_keys_str_mv	AT mehtanikhil thiolcompoundspreconditioningandorientationofexplantsimportantfactorsaffectingregenerationfromcotyledonsoflegumecropsesbaniaaculeata AT raopriyanka thiolcompoundspreconditioningandorientationofexplantsimportantfactorsaffectingregenerationfromcotyledonsoflegumecropsesbaniaaculeata AT sainiraman thiolcompoundspreconditioningandorientationofexplantsimportantfactorsaffectingregenerationfromcotyledonsoflegumecropsesbaniaaculeata
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Thiol Compounds, Pre-Conditioning and Orientation of Explants – Important Factors Affecting Regeneration from Cotyledons of Legume Crop Sesbania Aculeata

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