Rhizobacterial community structure in grafted tomato plants infected by Ralstonia solanacearum

Abstract. Navitasari L, Joko T, Murti RH, Arwiyanto T. 2020. Rhizobacterial community structure in grafted tomato plants infected by Ralstonia solanacearum. Biodiversitas 21: 4888-4895. Bacterial wilt disease caused by Ralstonia solanacearum is a devastating soil-borne vascular disease of tomato lea...

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Autores principales:	Lisa Navitasari, TRI JOKO, RUDI HARI MURTI, TRIWIDODO ARWIYANTO
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	bacterial wilt, disease intensity, grafting, predominant rhizobacteria Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/c0af039de1824751b228cceb267403dc
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spelling	oai:doaj.org-article:c0af039de1824751b228cceb267403dc2021-11-22T00:51:08ZRhizobacterial community structure in grafted tomato plants infected by Ralstonia solanacearum1412-033X2085-472210.13057/biodiv/d211055https://doaj.org/article/c0af039de1824751b228cceb267403dc2020-09-01T00:00:00Zhttps://smujo.id/biodiv/article/view/5714https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Navitasari L, Joko T, Murti RH, Arwiyanto T. 2020. Rhizobacterial community structure in grafted tomato plants infected by Ralstonia solanacearum. Biodiversitas 21: 4888-4895. Bacterial wilt disease caused by Ralstonia solanacearum is a devastating soil-borne vascular disease of tomato leading to a 100% yield loss. One of the alternatives to suppress the infestation of R. solanacearum infestation is the application of grafting techniques, which has been studied and successfully practiced by tomato growers. However, the infestation mode of R. solanacearum and the rhizobacterial community structure in grafted tomato plants are poorly reported. In this study, the rhizobacterial community structure in grafted tomato plants infected by R. solanacearum was investigated. The experiment was conducted on tomato germplasms with the implementation of tube grafting using resistant rootstocks (Amelia from Indonesia, H.7996 from Asian Vegetable Research Development Center/AVRDC) and susceptible scion (Servo from Indonesia). The rhizobacterial community structure was analyzed by metagenomic study under 16S rRNA genes sequencing with a distinct region (16SV3-V4) that was amplified using a specific primer (16SV4: 515F-806R) 5’-GTGCCAGCMGCCGCGGTAA and 5’GGACTACHVHHHTWTCTAAT. The results indicated that the grafted tomato plants and resistant rootstocks that were infected by R. solanacearum showed significantly lower intensity of bacterial wilt disease compared to the susceptible scion. The rhizobacterial community structure in the grafted tomato plants infected by R. solanacearum was indicated by predominant phyla of Proteobacteria, Firmicutes, and Actinobacteria with dominant genera of Pseudomonas and Bacillus. Besides, significant difference was also indicated by species of Geitlerinema sp. in the grafted tomatoes infected by R. solanacearum.Lisa NavitasariTRI JOKORUDI HARI MURTITRIWIDODO ARWIYANTOMBI & UNS Soloarticlebacterial wilt, disease intensity, grafting, predominant rhizobacteriaBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacterial wilt, disease intensity, grafting, predominant rhizobacteria Biology (General) QH301-705.5
spellingShingle	bacterial wilt, disease intensity, grafting, predominant rhizobacteria Biology (General) QH301-705.5 Lisa Navitasari TRI JOKO RUDI HARI MURTI TRIWIDODO ARWIYANTO Rhizobacterial community structure in grafted tomato plants infected by Ralstonia solanacearum
description	Abstract. Navitasari L, Joko T, Murti RH, Arwiyanto T. 2020. Rhizobacterial community structure in grafted tomato plants infected by Ralstonia solanacearum. Biodiversitas 21: 4888-4895. Bacterial wilt disease caused by Ralstonia solanacearum is a devastating soil-borne vascular disease of tomato leading to a 100% yield loss. One of the alternatives to suppress the infestation of R. solanacearum infestation is the application of grafting techniques, which has been studied and successfully practiced by tomato growers. However, the infestation mode of R. solanacearum and the rhizobacterial community structure in grafted tomato plants are poorly reported. In this study, the rhizobacterial community structure in grafted tomato plants infected by R. solanacearum was investigated. The experiment was conducted on tomato germplasms with the implementation of tube grafting using resistant rootstocks (Amelia from Indonesia, H.7996 from Asian Vegetable Research Development Center/AVRDC) and susceptible scion (Servo from Indonesia). The rhizobacterial community structure was analyzed by metagenomic study under 16S rRNA genes sequencing with a distinct region (16SV3-V4) that was amplified using a specific primer (16SV4: 515F-806R) 5’-GTGCCAGCMGCCGCGGTAA and 5’GGACTACHVHHHTWTCTAAT. The results indicated that the grafted tomato plants and resistant rootstocks that were infected by R. solanacearum showed significantly lower intensity of bacterial wilt disease compared to the susceptible scion. The rhizobacterial community structure in the grafted tomato plants infected by R. solanacearum was indicated by predominant phyla of Proteobacteria, Firmicutes, and Actinobacteria with dominant genera of Pseudomonas and Bacillus. Besides, significant difference was also indicated by species of Geitlerinema sp. in the grafted tomatoes infected by R. solanacearum.
format	article
author	Lisa Navitasari TRI JOKO RUDI HARI MURTI TRIWIDODO ARWIYANTO
author_facet	Lisa Navitasari TRI JOKO RUDI HARI MURTI TRIWIDODO ARWIYANTO
author_sort	Lisa Navitasari
title	Rhizobacterial community structure in grafted tomato plants infected by Ralstonia solanacearum
title_short	Rhizobacterial community structure in grafted tomato plants infected by Ralstonia solanacearum
title_full	Rhizobacterial community structure in grafted tomato plants infected by Ralstonia solanacearum
title_fullStr	Rhizobacterial community structure in grafted tomato plants infected by Ralstonia solanacearum
title_full_unstemmed	Rhizobacterial community structure in grafted tomato plants infected by Ralstonia solanacearum
title_sort	rhizobacterial community structure in grafted tomato plants infected by ralstonia solanacearum
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/c0af039de1824751b228cceb267403dc
work_keys_str_mv	AT lisanavitasari rhizobacterialcommunitystructureingraftedtomatoplantsinfectedbyralstoniasolanacearum AT trijoko rhizobacterialcommunitystructureingraftedtomatoplantsinfectedbyralstoniasolanacearum AT rudiharimurti rhizobacterialcommunitystructureingraftedtomatoplantsinfectedbyralstoniasolanacearum AT triwidodoarwiyanto rhizobacterialcommunitystructureingraftedtomatoplantsinfectedbyralstoniasolanacearum
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Rhizobacterial community structure in grafted tomato plants infected by Ralstonia solanacearum

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