Metabolomic Characterization of Cerebrospinal Fluid from Intracranial Bacterial Infection Pediatric Patients: A Pilot Study

Intracranial bacterial infection remains a major cause of morbidity and mortality in neurosurgical cases. Metabolomic profiling of cerebrospinal fluid (CSF) holds great promise to gain insights into the pathogenesis of central neural system (CNS) bacterial infections. In this pilot study, we analyze...

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Autores principales:	Yiwen Wang, Yu Liu, Ruoping Chen, Liang Qiao
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cerebrospinal fluid central neural system infection mass spectrometry metabolomics metabolic pathway Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/c0b2d4d0b8c94ff8ae15232b345a772e
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spelling	oai:doaj.org-article:c0b2d4d0b8c94ff8ae15232b345a772e2021-11-25T18:27:59ZMetabolomic Characterization of Cerebrospinal Fluid from Intracranial Bacterial Infection Pediatric Patients: A Pilot Study10.3390/molecules262268711420-3049https://doaj.org/article/c0b2d4d0b8c94ff8ae15232b345a772e2021-11-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/22/6871https://doaj.org/toc/1420-3049Intracranial bacterial infection remains a major cause of morbidity and mortality in neurosurgical cases. Metabolomic profiling of cerebrospinal fluid (CSF) holds great promise to gain insights into the pathogenesis of central neural system (CNS) bacterial infections. In this pilot study, we analyzed the metabolites in CSF of CNS infection patients and controls in a pseudo-targeted manner, aiming at elucidating the metabolic dysregulation in response to postoperative intracranial bacterial infection of pediatric cases. Untargeted analysis uncovered 597 metabolites, and screened out 206 differential metabolites in case of infection. Targeted verification and pathway analysis filtered out the glycolysis, amino acids metabolism and purine metabolism pathways as potential pathological pathways. These perturbed pathways are involved in the infection-induced oxidative stress and immune response. Characterization of the infection-induced metabolic changes can provide robust biomarkers of CNS bacterial infection for clinical diagnosis, novel pathways for pathological investigation, and new targets for treatment.Yiwen WangYu LiuRuoping ChenLiang QiaoMDPI AGarticlecerebrospinal fluidcentral neural system infectionmass spectrometrymetabolomicsmetabolic pathwayOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6871, p 6871 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cerebrospinal fluid central neural system infection mass spectrometry metabolomics metabolic pathway Organic chemistry QD241-441
spellingShingle	cerebrospinal fluid central neural system infection mass spectrometry metabolomics metabolic pathway Organic chemistry QD241-441 Yiwen Wang Yu Liu Ruoping Chen Liang Qiao Metabolomic Characterization of Cerebrospinal Fluid from Intracranial Bacterial Infection Pediatric Patients: A Pilot Study
description	Intracranial bacterial infection remains a major cause of morbidity and mortality in neurosurgical cases. Metabolomic profiling of cerebrospinal fluid (CSF) holds great promise to gain insights into the pathogenesis of central neural system (CNS) bacterial infections. In this pilot study, we analyzed the metabolites in CSF of CNS infection patients and controls in a pseudo-targeted manner, aiming at elucidating the metabolic dysregulation in response to postoperative intracranial bacterial infection of pediatric cases. Untargeted analysis uncovered 597 metabolites, and screened out 206 differential metabolites in case of infection. Targeted verification and pathway analysis filtered out the glycolysis, amino acids metabolism and purine metabolism pathways as potential pathological pathways. These perturbed pathways are involved in the infection-induced oxidative stress and immune response. Characterization of the infection-induced metabolic changes can provide robust biomarkers of CNS bacterial infection for clinical diagnosis, novel pathways for pathological investigation, and new targets for treatment.
format	article
author	Yiwen Wang Yu Liu Ruoping Chen Liang Qiao
author_facet	Yiwen Wang Yu Liu Ruoping Chen Liang Qiao
author_sort	Yiwen Wang
title	Metabolomic Characterization of Cerebrospinal Fluid from Intracranial Bacterial Infection Pediatric Patients: A Pilot Study
title_short	Metabolomic Characterization of Cerebrospinal Fluid from Intracranial Bacterial Infection Pediatric Patients: A Pilot Study
title_full	Metabolomic Characterization of Cerebrospinal Fluid from Intracranial Bacterial Infection Pediatric Patients: A Pilot Study
title_fullStr	Metabolomic Characterization of Cerebrospinal Fluid from Intracranial Bacterial Infection Pediatric Patients: A Pilot Study
title_full_unstemmed	Metabolomic Characterization of Cerebrospinal Fluid from Intracranial Bacterial Infection Pediatric Patients: A Pilot Study
title_sort	metabolomic characterization of cerebrospinal fluid from intracranial bacterial infection pediatric patients: a pilot study
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/c0b2d4d0b8c94ff8ae15232b345a772e
work_keys_str_mv	AT yiwenwang metabolomiccharacterizationofcerebrospinalfluidfromintracranialbacterialinfectionpediatricpatientsapilotstudy AT yuliu metabolomiccharacterizationofcerebrospinalfluidfromintracranialbacterialinfectionpediatricpatientsapilotstudy AT ruopingchen metabolomiccharacterizationofcerebrospinalfluidfromintracranialbacterialinfectionpediatricpatientsapilotstudy AT liangqiao metabolomiccharacterizationofcerebrospinalfluidfromintracranialbacterialinfectionpediatricpatientsapilotstudy
_version_	1718411155597164544

Metabolomic Characterization of Cerebrospinal Fluid from Intracranial Bacterial Infection Pediatric Patients: A Pilot Study

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