Rapid and sensitive detection of rare cancer cells by the coupling of immunomagnetic nanoparticle separation with ELISA analysis

Hao-Yuan Cheng,1 Lee-Jene Lai,2 Fu-Hsiang Ko11Department of Materials Science and Engineering, National Chiao Tung University, Taiwan, Republic of China; 2National Synchrotron Radiation Research Center, Taiwan, Republic of ChinaAbstract: This study presents a rapid and sensitive method for detecting...

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Autores principales:	Ko FH, Lai LJ, Cheng HY
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/c0b5e4ee390e49c9970285c1f9a418bb
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spelling	oai:doaj.org-article:c0b5e4ee390e49c9970285c1f9a418bb2021-12-02T05:26:37ZRapid and sensitive detection of rare cancer cells by the coupling of immunomagnetic nanoparticle separation with ELISA analysis1176-91141178-2013https://doaj.org/article/c0b5e4ee390e49c9970285c1f9a418bb2012-06-01T00:00:00Zhttp://www.dovepress.com/rapid-and-sensitive-detection-of-rare-cancer-cells-by-the-coupling-of--a10135https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Hao-Yuan Cheng,1 Lee-Jene Lai,2 Fu-Hsiang Ko11Department of Materials Science and Engineering, National Chiao Tung University, Taiwan, Republic of China; 2National Synchrotron Radiation Research Center, Taiwan, Republic of ChinaAbstract: This study presents a rapid and sensitive method for detecting cancer cells occurring at low concentration. The method involves the simultaneous detection of two biomarkers of T helper cancer cells. One biomarker conjugates with immunofunctionalized magnetic nanoparticles (MNPs), enabling the separation of the T helper cells from a mixed population of cells. The other biomarker is used for detection during enzyme-linked immunosorbent assay (ELISA) analysis. The specific T helper cells can be quantified according to their ELISA absorbance values following magnetic separation. The experimental results demonstrate that immunofunctionalized MNPs can function as magnetic sensors and separate specific T helper cells from a mixed population with high efficiency and high specificity. Coupled with the ELISA technique, the immunofunctionalized MNPs can simultaneously detect rare cells. Results indicated increasing absorbance with increasing T cell number (from 10 to 106). The total detection time was less than 15 minutes, even at a low T cell count. The advantages of the proposed method for detecting specific cells at low concentration include ease of preparation, low cost, rapid detection, and high sensitivity. The proposed system can be adopted to detect circulating tumor cells in early tumor stages for diagnostic or prognostic purposes.Keywords: ELISA, magnetic nanoparticles, immunoassay, cancer cellKo FHLai LJCheng HYDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 2967-2973 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Ko FH Lai LJ Cheng HY Rapid and sensitive detection of rare cancer cells by the coupling of immunomagnetic nanoparticle separation with ELISA analysis
description	Hao-Yuan Cheng,1 Lee-Jene Lai,2 Fu-Hsiang Ko11Department of Materials Science and Engineering, National Chiao Tung University, Taiwan, Republic of China; 2National Synchrotron Radiation Research Center, Taiwan, Republic of ChinaAbstract: This study presents a rapid and sensitive method for detecting cancer cells occurring at low concentration. The method involves the simultaneous detection of two biomarkers of T helper cancer cells. One biomarker conjugates with immunofunctionalized magnetic nanoparticles (MNPs), enabling the separation of the T helper cells from a mixed population of cells. The other biomarker is used for detection during enzyme-linked immunosorbent assay (ELISA) analysis. The specific T helper cells can be quantified according to their ELISA absorbance values following magnetic separation. The experimental results demonstrate that immunofunctionalized MNPs can function as magnetic sensors and separate specific T helper cells from a mixed population with high efficiency and high specificity. Coupled with the ELISA technique, the immunofunctionalized MNPs can simultaneously detect rare cells. Results indicated increasing absorbance with increasing T cell number (from 10 to 106). The total detection time was less than 15 minutes, even at a low T cell count. The advantages of the proposed method for detecting specific cells at low concentration include ease of preparation, low cost, rapid detection, and high sensitivity. The proposed system can be adopted to detect circulating tumor cells in early tumor stages for diagnostic or prognostic purposes.Keywords: ELISA, magnetic nanoparticles, immunoassay, cancer cell
format	article
author	Ko FH Lai LJ Cheng HY
author_facet	Ko FH Lai LJ Cheng HY
author_sort	Ko FH
title	Rapid and sensitive detection of rare cancer cells by the coupling of immunomagnetic nanoparticle separation with ELISA analysis
title_short	Rapid and sensitive detection of rare cancer cells by the coupling of immunomagnetic nanoparticle separation with ELISA analysis
title_full	Rapid and sensitive detection of rare cancer cells by the coupling of immunomagnetic nanoparticle separation with ELISA analysis
title_fullStr	Rapid and sensitive detection of rare cancer cells by the coupling of immunomagnetic nanoparticle separation with ELISA analysis
title_full_unstemmed	Rapid and sensitive detection of rare cancer cells by the coupling of immunomagnetic nanoparticle separation with ELISA analysis
title_sort	rapid and sensitive detection of rare cancer cells by the coupling of immunomagnetic nanoparticle separation with elisa analysis
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/c0b5e4ee390e49c9970285c1f9a418bb
work_keys_str_mv	AT kofh rapidandsensitivedetectionofrarecancercellsbythecouplingofimmunomagneticnanoparticleseparationwithelisaanalysis AT lailj rapidandsensitivedetectionofrarecancercellsbythecouplingofimmunomagneticnanoparticleseparationwithelisaanalysis AT chenghy rapidandsensitivedetectionofrarecancercellsbythecouplingofimmunomagneticnanoparticleseparationwithelisaanalysis
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Rapid and sensitive detection of rare cancer cells by the coupling of immunomagnetic nanoparticle separation with ELISA analysis

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