Loss of FYCO1 leads to cataract formation

Abstract Autophagy is a degradation process of cytoplasmic proteins and organelles trafficked to degradation vesicles known as autophagosomes. The conversion of LC3-I to LC3-II is an essential step of autophagosome formation, and FYCO1 is a LC3-binding protein that mediates autophagosome transport....

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Autores principales:	Kiyotoshi Satoh, Yukitoshi Takemura, Motohiko Satoh, Kiyokazu Ozaki, Shunichiro Kubota
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c0b8ac297cb349e38c508fc1040412f8
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spelling	oai:doaj.org-article:c0b8ac297cb349e38c508fc1040412f82021-12-02T16:32:13ZLoss of FYCO1 leads to cataract formation10.1038/s41598-021-93110-12045-2322https://doaj.org/article/c0b8ac297cb349e38c508fc1040412f82021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93110-1https://doaj.org/toc/2045-2322Abstract Autophagy is a degradation process of cytoplasmic proteins and organelles trafficked to degradation vesicles known as autophagosomes. The conversion of LC3-I to LC3-II is an essential step of autophagosome formation, and FYCO1 is a LC3-binding protein that mediates autophagosome transport. The p62 protein also directly binds to LC3 and is degraded by autophagy. In the present study, we demonstrated that disrupting the FYCO1 gene in mice resulted in cataract formation. LC3 conversion decreased in eyes from FYCO1 knockout mice. Further, FYCO1 interacted with αA- and αB-crystallin, as demonstrated by yeast two-hybrid screening and immunoprecipitation analyses. In eyes from knockout mice, the soluble forms of αA- and αB-crystallin, the lens’s major protein components, decreased. In addition, p62 accumulated in eyes from FYCO1 knockout mice. Collectively, these findings suggested that FYCO1 recruited damaged α-crystallin into autophagosomes to protect lens cells from cataract formation.Kiyotoshi SatohYukitoshi TakemuraMotohiko SatohKiyokazu OzakiShunichiro KubotaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kiyotoshi Satoh Yukitoshi Takemura Motohiko Satoh Kiyokazu Ozaki Shunichiro Kubota Loss of FYCO1 leads to cataract formation
description	Abstract Autophagy is a degradation process of cytoplasmic proteins and organelles trafficked to degradation vesicles known as autophagosomes. The conversion of LC3-I to LC3-II is an essential step of autophagosome formation, and FYCO1 is a LC3-binding protein that mediates autophagosome transport. The p62 protein also directly binds to LC3 and is degraded by autophagy. In the present study, we demonstrated that disrupting the FYCO1 gene in mice resulted in cataract formation. LC3 conversion decreased in eyes from FYCO1 knockout mice. Further, FYCO1 interacted with αA- and αB-crystallin, as demonstrated by yeast two-hybrid screening and immunoprecipitation analyses. In eyes from knockout mice, the soluble forms of αA- and αB-crystallin, the lens’s major protein components, decreased. In addition, p62 accumulated in eyes from FYCO1 knockout mice. Collectively, these findings suggested that FYCO1 recruited damaged α-crystallin into autophagosomes to protect lens cells from cataract formation.
format	article
author	Kiyotoshi Satoh Yukitoshi Takemura Motohiko Satoh Kiyokazu Ozaki Shunichiro Kubota
author_facet	Kiyotoshi Satoh Yukitoshi Takemura Motohiko Satoh Kiyokazu Ozaki Shunichiro Kubota
author_sort	Kiyotoshi Satoh
title	Loss of FYCO1 leads to cataract formation
title_short	Loss of FYCO1 leads to cataract formation
title_full	Loss of FYCO1 leads to cataract formation
title_fullStr	Loss of FYCO1 leads to cataract formation
title_full_unstemmed	Loss of FYCO1 leads to cataract formation
title_sort	loss of fyco1 leads to cataract formation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c0b8ac297cb349e38c508fc1040412f8
work_keys_str_mv	AT kiyotoshisatoh lossoffyco1leadstocataractformation AT yukitoshitakemura lossoffyco1leadstocataractformation AT motohikosatoh lossoffyco1leadstocataractformation AT kiyokazuozaki lossoffyco1leadstocataractformation AT shunichirokubota lossoffyco1leadstocataractformation
_version_	1718383832829263872

Loss of FYCO1 leads to cataract formation

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