Dentin Matrix Protein 1 on Titanium Surface Facilitates Osteogenic Differentiation of Stem Cells

Dentin matrix protein 1 (DMP1) contains a large number of acidic domains, multiple phosphorylation sites, a functional arginine-glycine-aspartate (RGD) motif, and a DNA binding domain, and has been shown to play essential regulatory function in dentin and bone mineralization. DMP1 could also orchest...

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Autores principales:	Suchada Kongkiatkamon, Amsaveni Ramachandran, Kent L. Knoernschild, Stephen D. Campbell, Cortino Sukotjo, Anne George
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	dental implant titanium surface modification DMP1 stem cell Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/c0bebb65637e4a838b807a8bb2a36359
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spelling	oai:doaj.org-article:c0bebb65637e4a838b807a8bb2a363592021-11-25T18:27:00ZDentin Matrix Protein 1 on Titanium Surface Facilitates Osteogenic Differentiation of Stem Cells10.3390/molecules262267561420-3049https://doaj.org/article/c0bebb65637e4a838b807a8bb2a363592021-11-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/22/6756https://doaj.org/toc/1420-3049Dentin matrix protein 1 (DMP1) contains a large number of acidic domains, multiple phosphorylation sites, a functional arginine-glycine-aspartate (RGD) motif, and a DNA binding domain, and has been shown to play essential regulatory function in dentin and bone mineralization. DMP1 could also orchestrate bone matrix formation, but the ability of DMP1 on Ti to human mesenchymal stem cell (hMSC) conversion to osteoblasts has not been studied. There is importance to test if the DMP1 coated Ti surface would promote cell migration and attachment to the metal surface and promote the differentiation of the attached stem cells to an osteogenic lineage. This study aimed to study the human mesenchymal stem cells (hMSCs) attachment and proliferation on DMP1 coated titanium (Ti) disks compared to non-coated disks, and to assess possible osteoblastic differentiation of attached hMSCs. Sixty-eight Ti disks were divided into two groups. Group 1 disks were coated with dentin matrix protein 1 and group 2 disks served as control. Assessment with light microscopy was used to verify hMSC attachment and proliferation. Cell viability was confirmed through fluorescence microscopy and mitochondrial dehydrogenase activity. Real-time polymerase chain reaction analysis was done to study the gene expression. The proliferation assay showed significantly greater cell proliferation with DMP1 coated disks compared to the control group (<i>p</i>-value < 0.001). Cell vitality analysis showed a greater density of live cells on DMP1 coated disks compared to the control group. Alkaline phosphatase staining revealed higher enzyme activity on DMP1 coated disks and showed itself to be significantly higher than the control group (<i>p</i>-value < 0.001). von Kossa staining revealed higher positive areas for mineralized deposits on DMP1 coated disks than the control group (<i>p</i>-value < 0.05). Gene expression analysis confirmed upregulation of runt-related transcription factor 2, osteoprotegerin, osteocalcin, osteopontin, and alkaline phosphatase on DMP1 coated disks (<i>p</i>-value < 0.001). The dentin matrix protein promoted the adhesion, proliferation, facilitation differentiation of hMSC, and mineralized matrix formation.Suchada KongkiatkamonAmsaveni RamachandranKent L. KnoernschildStephen D. CampbellCortino SukotjoAnne GeorgeMDPI AGarticledental implanttitaniumsurface modificationDMP1stem cellOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6756, p 6756 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	dental implant titanium surface modification DMP1 stem cell Organic chemistry QD241-441
spellingShingle	dental implant titanium surface modification DMP1 stem cell Organic chemistry QD241-441 Suchada Kongkiatkamon Amsaveni Ramachandran Kent L. Knoernschild Stephen D. Campbell Cortino Sukotjo Anne George Dentin Matrix Protein 1 on Titanium Surface Facilitates Osteogenic Differentiation of Stem Cells
description	Dentin matrix protein 1 (DMP1) contains a large number of acidic domains, multiple phosphorylation sites, a functional arginine-glycine-aspartate (RGD) motif, and a DNA binding domain, and has been shown to play essential regulatory function in dentin and bone mineralization. DMP1 could also orchestrate bone matrix formation, but the ability of DMP1 on Ti to human mesenchymal stem cell (hMSC) conversion to osteoblasts has not been studied. There is importance to test if the DMP1 coated Ti surface would promote cell migration and attachment to the metal surface and promote the differentiation of the attached stem cells to an osteogenic lineage. This study aimed to study the human mesenchymal stem cells (hMSCs) attachment and proliferation on DMP1 coated titanium (Ti) disks compared to non-coated disks, and to assess possible osteoblastic differentiation of attached hMSCs. Sixty-eight Ti disks were divided into two groups. Group 1 disks were coated with dentin matrix protein 1 and group 2 disks served as control. Assessment with light microscopy was used to verify hMSC attachment and proliferation. Cell viability was confirmed through fluorescence microscopy and mitochondrial dehydrogenase activity. Real-time polymerase chain reaction analysis was done to study the gene expression. The proliferation assay showed significantly greater cell proliferation with DMP1 coated disks compared to the control group (<i>p</i>-value < 0.001). Cell vitality analysis showed a greater density of live cells on DMP1 coated disks compared to the control group. Alkaline phosphatase staining revealed higher enzyme activity on DMP1 coated disks and showed itself to be significantly higher than the control group (<i>p</i>-value < 0.001). von Kossa staining revealed higher positive areas for mineralized deposits on DMP1 coated disks than the control group (<i>p</i>-value < 0.05). Gene expression analysis confirmed upregulation of runt-related transcription factor 2, osteoprotegerin, osteocalcin, osteopontin, and alkaline phosphatase on DMP1 coated disks (<i>p</i>-value < 0.001). The dentin matrix protein promoted the adhesion, proliferation, facilitation differentiation of hMSC, and mineralized matrix formation.
format	article
author	Suchada Kongkiatkamon Amsaveni Ramachandran Kent L. Knoernschild Stephen D. Campbell Cortino Sukotjo Anne George
author_facet	Suchada Kongkiatkamon Amsaveni Ramachandran Kent L. Knoernschild Stephen D. Campbell Cortino Sukotjo Anne George
author_sort	Suchada Kongkiatkamon
title	Dentin Matrix Protein 1 on Titanium Surface Facilitates Osteogenic Differentiation of Stem Cells
title_short	Dentin Matrix Protein 1 on Titanium Surface Facilitates Osteogenic Differentiation of Stem Cells
title_full	Dentin Matrix Protein 1 on Titanium Surface Facilitates Osteogenic Differentiation of Stem Cells
title_fullStr	Dentin Matrix Protein 1 on Titanium Surface Facilitates Osteogenic Differentiation of Stem Cells
title_full_unstemmed	Dentin Matrix Protein 1 on Titanium Surface Facilitates Osteogenic Differentiation of Stem Cells
title_sort	dentin matrix protein 1 on titanium surface facilitates osteogenic differentiation of stem cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/c0bebb65637e4a838b807a8bb2a36359
work_keys_str_mv	AT suchadakongkiatkamon dentinmatrixprotein1ontitaniumsurfacefacilitatesosteogenicdifferentiationofstemcells AT amsaveniramachandran dentinmatrixprotein1ontitaniumsurfacefacilitatesosteogenicdifferentiationofstemcells AT kentlknoernschild dentinmatrixprotein1ontitaniumsurfacefacilitatesosteogenicdifferentiationofstemcells AT stephendcampbell dentinmatrixprotein1ontitaniumsurfacefacilitatesosteogenicdifferentiationofstemcells AT cortinosukotjo dentinmatrixprotein1ontitaniumsurfacefacilitatesosteogenicdifferentiationofstemcells AT annegeorge dentinmatrixprotein1ontitaniumsurfacefacilitatesosteogenicdifferentiationofstemcells
_version_	1718411155820511232

Dentin Matrix Protein 1 on Titanium Surface Facilitates Osteogenic Differentiation of Stem Cells

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