Label-free imaging of lipid depositions in C. elegans using third-harmonic generation microscopy.

Elucidation of the molecular mechanisms regulating lipid storage and metabolism is essential for mitigating excess adiposity and obesity, which has been associated with increased prevalence of severe pathological conditions such as cardiovascular disorders and type II diabetes, worldwide. However, i...

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Autores principales:	George J Tserevelakis, Evgenia V Megalou, George Filippidis, Barbara Petanidou, Costas Fotakis, Nektarios Tavernarakis
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c0c390e929fb416b85763c38e7e43157
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spelling	oai:doaj.org-article:c0c390e929fb416b85763c38e7e431572021-11-18T08:39:05ZLabel-free imaging of lipid depositions in C. elegans using third-harmonic generation microscopy.1932-620310.1371/journal.pone.0084431https://doaj.org/article/c0c390e929fb416b85763c38e7e431572014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24392137/?tool=EBIhttps://doaj.org/toc/1932-6203Elucidation of the molecular mechanisms regulating lipid storage and metabolism is essential for mitigating excess adiposity and obesity, which has been associated with increased prevalence of severe pathological conditions such as cardiovascular disorders and type II diabetes, worldwide. However, imaging fatty acid distribution and dynamics in vivo, at the cellular or organismal level is challenging. We developed a label-free method for visualizing lipid depositions in vivo, based on third harmonic generation (THG) microscopy. THG imaging requires a single pulsed-laser light source, alleviating the technical challenges of implementing coherent anti-Stokes Raman scattering spectroscopy (CARS) to detect fat stores in living cells. We demonstrate that THG can be used to efficiently and reliably visualize lipid droplets in Caenorhabditis elegans. Thus, THG microscopy offers a versatile alternative to fluorescence and dye-based approaches for lipid biology research.George J TserevelakisEvgenia V MegalouGeorge FilippidisBarbara PetanidouCostas FotakisNektarios TavernarakisPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e84431 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q George J Tserevelakis Evgenia V Megalou George Filippidis Barbara Petanidou Costas Fotakis Nektarios Tavernarakis Label-free imaging of lipid depositions in C. elegans using third-harmonic generation microscopy.
description	Elucidation of the molecular mechanisms regulating lipid storage and metabolism is essential for mitigating excess adiposity and obesity, which has been associated with increased prevalence of severe pathological conditions such as cardiovascular disorders and type II diabetes, worldwide. However, imaging fatty acid distribution and dynamics in vivo, at the cellular or organismal level is challenging. We developed a label-free method for visualizing lipid depositions in vivo, based on third harmonic generation (THG) microscopy. THG imaging requires a single pulsed-laser light source, alleviating the technical challenges of implementing coherent anti-Stokes Raman scattering spectroscopy (CARS) to detect fat stores in living cells. We demonstrate that THG can be used to efficiently and reliably visualize lipid droplets in Caenorhabditis elegans. Thus, THG microscopy offers a versatile alternative to fluorescence and dye-based approaches for lipid biology research.
format	article
author	George J Tserevelakis Evgenia V Megalou George Filippidis Barbara Petanidou Costas Fotakis Nektarios Tavernarakis
author_facet	George J Tserevelakis Evgenia V Megalou George Filippidis Barbara Petanidou Costas Fotakis Nektarios Tavernarakis
author_sort	George J Tserevelakis
title	Label-free imaging of lipid depositions in C. elegans using third-harmonic generation microscopy.
title_short	Label-free imaging of lipid depositions in C. elegans using third-harmonic generation microscopy.
title_full	Label-free imaging of lipid depositions in C. elegans using third-harmonic generation microscopy.
title_fullStr	Label-free imaging of lipid depositions in C. elegans using third-harmonic generation microscopy.
title_full_unstemmed	Label-free imaging of lipid depositions in C. elegans using third-harmonic generation microscopy.
title_sort	label-free imaging of lipid depositions in c. elegans using third-harmonic generation microscopy.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/c0c390e929fb416b85763c38e7e43157
work_keys_str_mv	AT georgejtserevelakis labelfreeimagingoflipiddepositionsincelegansusingthirdharmonicgenerationmicroscopy AT evgeniavmegalou labelfreeimagingoflipiddepositionsincelegansusingthirdharmonicgenerationmicroscopy AT georgefilippidis labelfreeimagingoflipiddepositionsincelegansusingthirdharmonicgenerationmicroscopy AT barbarapetanidou labelfreeimagingoflipiddepositionsincelegansusingthirdharmonicgenerationmicroscopy AT costasfotakis labelfreeimagingoflipiddepositionsincelegansusingthirdharmonicgenerationmicroscopy AT nektariostavernarakis labelfreeimagingoflipiddepositionsincelegansusingthirdharmonicgenerationmicroscopy
_version_	1718421525921529856

Label-free imaging of lipid depositions in C. elegans using third-harmonic generation microscopy.

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