Involvement of cancer-derived EMT cells in the accumulation of 18F-fluorodeoxyglucose in the hypoxic cancer microenvironment

Abstract A high rate of glycolysis, one of the most common features of cancer, is used in positron emission tomography (PET) imaging to visualize tumor tissues using 18F-fluorodeoxyglucose (18F-FDG). Heterogeneous intratumoral distribution of 18F-FDG in tissues has been established in some types of...

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Autores principales:	Sachi Sugita, Masanori Yamato, Toshimitsu Hatabu, Yosky Kataoka
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c0ca0590a75d41e6951ed60788a9057f
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spelling	oai:doaj.org-article:c0ca0590a75d41e6951ed60788a9057f2021-12-02T14:59:06ZInvolvement of cancer-derived EMT cells in the accumulation of 18F-fluorodeoxyglucose in the hypoxic cancer microenvironment10.1038/s41598-021-88414-12045-2322https://doaj.org/article/c0ca0590a75d41e6951ed60788a9057f2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88414-1https://doaj.org/toc/2045-2322Abstract A high rate of glycolysis, one of the most common features of cancer, is used in positron emission tomography (PET) imaging to visualize tumor tissues using 18F-fluorodeoxyglucose (18F-FDG). Heterogeneous intratumoral distribution of 18F-FDG in tissues has been established in some types of cancer, and the maximum standardized uptake value (SUVmax) has been correlated with poor prognosis. However, the phenotype of cells that show high 18F-FDG accumulation in tumors remains unknown. Here, we combined quantitative micro-autoradiography with fluorescence immunohistochemistry to simultaneously visualize 18F-FDG distribution, the expression of multiple proteins, and hypoxic regions in the cancer microenvironment of a human A431 xenograft tumor in C.B-17/Icr-scid/scid mice. We found that the highest 18F-FDG accumulation was in cancer-derived cells undergoing epithelial-mesenchymal transition (EMT) in hypoxic regions, implicating these regions as a major contributor to increased glucose metabolism, as measured by 18F-FDG-PET.Sachi SugitaMasanori YamatoToshimitsu HatabuYosky KataokaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sachi Sugita Masanori Yamato Toshimitsu Hatabu Yosky Kataoka Involvement of cancer-derived EMT cells in the accumulation of 18F-fluorodeoxyglucose in the hypoxic cancer microenvironment
description	Abstract A high rate of glycolysis, one of the most common features of cancer, is used in positron emission tomography (PET) imaging to visualize tumor tissues using 18F-fluorodeoxyglucose (18F-FDG). Heterogeneous intratumoral distribution of 18F-FDG in tissues has been established in some types of cancer, and the maximum standardized uptake value (SUVmax) has been correlated with poor prognosis. However, the phenotype of cells that show high 18F-FDG accumulation in tumors remains unknown. Here, we combined quantitative micro-autoradiography with fluorescence immunohistochemistry to simultaneously visualize 18F-FDG distribution, the expression of multiple proteins, and hypoxic regions in the cancer microenvironment of a human A431 xenograft tumor in C.B-17/Icr-scid/scid mice. We found that the highest 18F-FDG accumulation was in cancer-derived cells undergoing epithelial-mesenchymal transition (EMT) in hypoxic regions, implicating these regions as a major contributor to increased glucose metabolism, as measured by 18F-FDG-PET.
format	article
author	Sachi Sugita Masanori Yamato Toshimitsu Hatabu Yosky Kataoka
author_facet	Sachi Sugita Masanori Yamato Toshimitsu Hatabu Yosky Kataoka
author_sort	Sachi Sugita
title	Involvement of cancer-derived EMT cells in the accumulation of 18F-fluorodeoxyglucose in the hypoxic cancer microenvironment
title_short	Involvement of cancer-derived EMT cells in the accumulation of 18F-fluorodeoxyglucose in the hypoxic cancer microenvironment
title_full	Involvement of cancer-derived EMT cells in the accumulation of 18F-fluorodeoxyglucose in the hypoxic cancer microenvironment
title_fullStr	Involvement of cancer-derived EMT cells in the accumulation of 18F-fluorodeoxyglucose in the hypoxic cancer microenvironment
title_full_unstemmed	Involvement of cancer-derived EMT cells in the accumulation of 18F-fluorodeoxyglucose in the hypoxic cancer microenvironment
title_sort	involvement of cancer-derived emt cells in the accumulation of 18f-fluorodeoxyglucose in the hypoxic cancer microenvironment
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c0ca0590a75d41e6951ed60788a9057f
work_keys_str_mv	AT sachisugita involvementofcancerderivedemtcellsintheaccumulationof18ffluorodeoxyglucoseinthehypoxiccancermicroenvironment AT masanoriyamato involvementofcancerderivedemtcellsintheaccumulationof18ffluorodeoxyglucoseinthehypoxiccancermicroenvironment AT toshimitsuhatabu involvementofcancerderivedemtcellsintheaccumulationof18ffluorodeoxyglucoseinthehypoxiccancermicroenvironment AT yoskykataoka involvementofcancerderivedemtcellsintheaccumulationof18ffluorodeoxyglucoseinthehypoxiccancermicroenvironment
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Involvement of cancer-derived EMT cells in the accumulation of 18F-fluorodeoxyglucose in the hypoxic cancer microenvironment

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