An approach for reliably investigating hippocampal sharp wave-ripples in vitro.

<h4>Background</h4>Among the various hippocampal network patterns, sharp wave-ripples (SPW-R) are currently the mechanistically least understood. Although accurate information on synaptic interactions between the participating neurons is essential for comprehensive understanding of the n...

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Autores principales:	Nikolaus Maier, Genela Morris, Friedrich W Johenning, Dietmar Schmitz
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c0cbcb4adffe493c952c24c159e6dab3
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spelling	oai:doaj.org-article:c0cbcb4adffe493c952c24c159e6dab32021-11-25T06:20:33ZAn approach for reliably investigating hippocampal sharp wave-ripples in vitro.1932-620310.1371/journal.pone.0006925https://doaj.org/article/c0cbcb4adffe493c952c24c159e6dab32009-09-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19738897/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Among the various hippocampal network patterns, sharp wave-ripples (SPW-R) are currently the mechanistically least understood. Although accurate information on synaptic interactions between the participating neurons is essential for comprehensive understanding of the network function during complex activities like SPW-R, such knowledge is currently notably scarce.<h4>Methodology/principal findings</h4>We demonstrate an in vitro approach to SPW-R that offers a simple experimental tool allowing detailed analysis of mechanisms governing the sharp wave-state of the hippocampus. We combine interface storage of slices with modifications of a conventional submerged recording system and established in vitro SPW-R comparable to their in vivo counterpart. We show that slice storage in the interface chamber close to physiological temperature is the required condition to preserve network integrity that is necessary for the generation of SPW-R. Moreover, we demonstrate the utility of our method for studying synaptic and network properties of SPW-R, using electrophysiological and imaging methods that can only be applied in the submerged system.<h4>Conclusions/significance</h4>The approach presented here demonstrates a reliable and experimentally simple strategy for studying hippocampal sharp wave-ripples. Given its utility and easy application we expect our model to foster the generation of new insight into the network physiology underlying SPW-R.Nikolaus MaierGenela MorrisFriedrich W JohenningDietmar SchmitzPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 9, p e6925 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nikolaus Maier Genela Morris Friedrich W Johenning Dietmar Schmitz An approach for reliably investigating hippocampal sharp wave-ripples in vitro.
description	<h4>Background</h4>Among the various hippocampal network patterns, sharp wave-ripples (SPW-R) are currently the mechanistically least understood. Although accurate information on synaptic interactions between the participating neurons is essential for comprehensive understanding of the network function during complex activities like SPW-R, such knowledge is currently notably scarce.<h4>Methodology/principal findings</h4>We demonstrate an in vitro approach to SPW-R that offers a simple experimental tool allowing detailed analysis of mechanisms governing the sharp wave-state of the hippocampus. We combine interface storage of slices with modifications of a conventional submerged recording system and established in vitro SPW-R comparable to their in vivo counterpart. We show that slice storage in the interface chamber close to physiological temperature is the required condition to preserve network integrity that is necessary for the generation of SPW-R. Moreover, we demonstrate the utility of our method for studying synaptic and network properties of SPW-R, using electrophysiological and imaging methods that can only be applied in the submerged system.<h4>Conclusions/significance</h4>The approach presented here demonstrates a reliable and experimentally simple strategy for studying hippocampal sharp wave-ripples. Given its utility and easy application we expect our model to foster the generation of new insight into the network physiology underlying SPW-R.
format	article
author	Nikolaus Maier Genela Morris Friedrich W Johenning Dietmar Schmitz
author_facet	Nikolaus Maier Genela Morris Friedrich W Johenning Dietmar Schmitz
author_sort	Nikolaus Maier
title	An approach for reliably investigating hippocampal sharp wave-ripples in vitro.
title_short	An approach for reliably investigating hippocampal sharp wave-ripples in vitro.
title_full	An approach for reliably investigating hippocampal sharp wave-ripples in vitro.
title_fullStr	An approach for reliably investigating hippocampal sharp wave-ripples in vitro.
title_full_unstemmed	An approach for reliably investigating hippocampal sharp wave-ripples in vitro.
title_sort	approach for reliably investigating hippocampal sharp wave-ripples in vitro.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/c0cbcb4adffe493c952c24c159e6dab3
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An approach for reliably investigating hippocampal sharp wave-ripples in vitro.

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