In vitro effectiveness of an aqueous extract of Neem (Azadirachta indica A. Juss) leaves on bacteria causing healthcare associated infection in Valledupar

Background: Globally, the need to address strategies for preventing infections associated with health care has increased worldwide. In the city of Valledupar, Colombia, reports of bacteria resistant to chemical or enzymatic biocides in hospital environments and surfaces are increasingly frequent, e...

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Autores principales:	Aslenis Emidia Melo Rios, Hedilka Jimenez Rios, Bertilda Pedraza Claros, Jeraldin Castrillon Mejia, Ailyn Fuentes Arias
Formato:	article
Lenguaje:	EN
Publicado:	Universidad de Antioquia 2021
Materias:	Hospital infection antimicrobial Neem Tree cross infection Acinetobacter baumanni Staphylococcus epidermidis Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
Acceso en línea:	https://doaj.org/article/c0ced60a903244bba64ed0281c7c9fce
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spelling	oai:doaj.org-article:c0ced60a903244bba64ed0281c7c9fce2021-12-02T19:42:44ZIn vitro effectiveness of an aqueous extract of Neem (Azadirachta indica A. Juss) leaves on bacteria causing healthcare associated infection in Valledupar10.17533/udea.vitae.v27n3a050121-40042145-2660https://doaj.org/article/c0ced60a903244bba64ed0281c7c9fce2021-01-01T00:00:00Zhttps://revistas.udea.edu.co/index.php/vitae/article/view/341266https://doaj.org/toc/0121-4004https://doaj.org/toc/2145-2660 Background: Globally, the need to address strategies for preventing infections associated with health care has increased worldwide. In the city of Valledupar, Colombia, reports of bacteria resistant to chemical or enzymatic biocides in hospital environments and surfaces are increasingly frequent, evidencing the importance of conducting studies aimed at identifying alternative active ingredients for disinfectant products. Objective: Evaluate the in vitro effectiveness of Neem leaves extract over bacteria strains isolated from different areas and surfaces of a health institution in Valledupar, compared to disinfectants for hospital use, an enzymatic detergent, and a commercial chemical disinfectant. Methods: Biocidal activities on bacteria isolated from hospital surfaces, such as Acinetobacter baumanni, Bacillus subtilis, Enterobacter aerogenes, Staphylococcus aureus, Staphylococcus epidermidis, Micrococcus sp, and Stenotrophomonas malthophila were analyzed. The Neem leaves extract was evaluated at concentrations of 3, 4, and 5 % for each bacterium during 15-minute contact time, incubated at 37 °C for 18 hours. We compared two antimicrobial chemicals, a disinfectant (based on formaldehyde, cetrimide, and glutaraldehyde), and an enzymatic detergent (based on protease, lipase, and amylase). Results: The aqueous Neem extract did not show significant differences with the other treatments with 99.48 to 100% inhibition against bacteria of the species Acinetobacter baumanni, Enterobacter aerogenes, Staphylococcus aureus, and Micrococcus sp.; Bacillus subtilis, and Stenotrophomonas maltophilia strains were the most resistant strains inhibited by enzymatic detergent and disinfectant, respectively. None of the products evaluated were effective against all in vitrostrains. Conclusions: These data show Neem’s bacteriostatic properties, its potential in in-hospital products, and the need to combine different active ingredients in a disinfection plan. Aslenis Emidia Melo RiosHedilka Jimenez RiosBertilda Pedraza ClarosJeraldin Castrillon MejiaAilyn Fuentes AriasUniversidad de AntioquiaarticleHospital infectionantimicrobialNeem Treecross infectionAcinetobacter baumanniStaphylococcus epidermidisFood processing and manufactureTP368-456Pharmaceutical industryHD9665-9675ENVitae, Vol 27, Iss 3 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Hospital infection antimicrobial Neem Tree cross infection Acinetobacter baumanni Staphylococcus epidermidis Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
spellingShingle	Hospital infection antimicrobial Neem Tree cross infection Acinetobacter baumanni Staphylococcus epidermidis Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675 Aslenis Emidia Melo Rios Hedilka Jimenez Rios Bertilda Pedraza Claros Jeraldin Castrillon Mejia Ailyn Fuentes Arias In vitro effectiveness of an aqueous extract of Neem (Azadirachta indica A. Juss) leaves on bacteria causing healthcare associated infection in Valledupar
description	Background: Globally, the need to address strategies for preventing infections associated with health care has increased worldwide. In the city of Valledupar, Colombia, reports of bacteria resistant to chemical or enzymatic biocides in hospital environments and surfaces are increasingly frequent, evidencing the importance of conducting studies aimed at identifying alternative active ingredients for disinfectant products. Objective: Evaluate the in vitro effectiveness of Neem leaves extract over bacteria strains isolated from different areas and surfaces of a health institution in Valledupar, compared to disinfectants for hospital use, an enzymatic detergent, and a commercial chemical disinfectant. Methods: Biocidal activities on bacteria isolated from hospital surfaces, such as Acinetobacter baumanni, Bacillus subtilis, Enterobacter aerogenes, Staphylococcus aureus, Staphylococcus epidermidis, Micrococcus sp, and Stenotrophomonas malthophila were analyzed. The Neem leaves extract was evaluated at concentrations of 3, 4, and 5 % for each bacterium during 15-minute contact time, incubated at 37 °C for 18 hours. We compared two antimicrobial chemicals, a disinfectant (based on formaldehyde, cetrimide, and glutaraldehyde), and an enzymatic detergent (based on protease, lipase, and amylase). Results: The aqueous Neem extract did not show significant differences with the other treatments with 99.48 to 100% inhibition against bacteria of the species Acinetobacter baumanni, Enterobacter aerogenes, Staphylococcus aureus, and Micrococcus sp.; Bacillus subtilis, and Stenotrophomonas maltophilia strains were the most resistant strains inhibited by enzymatic detergent and disinfectant, respectively. None of the products evaluated were effective against all in vitrostrains. Conclusions: These data show Neem’s bacteriostatic properties, its potential in in-hospital products, and the need to combine different active ingredients in a disinfection plan.
format	article
author	Aslenis Emidia Melo Rios Hedilka Jimenez Rios Bertilda Pedraza Claros Jeraldin Castrillon Mejia Ailyn Fuentes Arias
author_facet	Aslenis Emidia Melo Rios Hedilka Jimenez Rios Bertilda Pedraza Claros Jeraldin Castrillon Mejia Ailyn Fuentes Arias
author_sort	Aslenis Emidia Melo Rios
title	In vitro effectiveness of an aqueous extract of Neem (Azadirachta indica A. Juss) leaves on bacteria causing healthcare associated infection in Valledupar
title_short	In vitro effectiveness of an aqueous extract of Neem (Azadirachta indica A. Juss) leaves on bacteria causing healthcare associated infection in Valledupar
title_full	In vitro effectiveness of an aqueous extract of Neem (Azadirachta indica A. Juss) leaves on bacteria causing healthcare associated infection in Valledupar
title_fullStr	In vitro effectiveness of an aqueous extract of Neem (Azadirachta indica A. Juss) leaves on bacteria causing healthcare associated infection in Valledupar
title_full_unstemmed	In vitro effectiveness of an aqueous extract of Neem (Azadirachta indica A. Juss) leaves on bacteria causing healthcare associated infection in Valledupar
title_sort	in vitro effectiveness of an aqueous extract of neem (azadirachta indica a. juss) leaves on bacteria causing healthcare associated infection in valledupar
publisher	Universidad de Antioquia
publishDate	2021
url	https://doaj.org/article/c0ced60a903244bba64ed0281c7c9fce
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In vitro effectiveness of an aqueous extract of Neem (Azadirachta indica A. Juss) leaves on bacteria causing healthcare associated infection in Valledupar

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