Multilayered Titanium Carbide MXene Film for Guided Bone Regeneration

Jiebing Zhang, Yu Fu, Anchun Mo State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, People’s Republic of ChinaCorrespondence: Anchun MoState Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu...

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Autores principales:	Zhang J, Fu Y, Mo A
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	ti3c2tx bone tissue engineering gbr osteoinductivity biocompatibility Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/c0dae6b216fd44e9977696c153d77237
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spelling	oai:doaj.org-article:c0dae6b216fd44e9977696c153d772372021-12-02T09:06:24ZMultilayered Titanium Carbide MXene Film for Guided Bone Regeneration1178-2013https://doaj.org/article/c0dae6b216fd44e9977696c153d772372019-12-01T00:00:00Zhttps://www.dovepress.com/multilayered-titanium-carbide-mxene-film-for-guided-bone-regeneration-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Jiebing Zhang, Yu Fu, Anchun Mo State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, People’s Republic of ChinaCorrespondence: Anchun MoState Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, People’s Republic of ChinaEmail moanchun@163.comPurpose: MXenes are two-dimensional (2D) materials that are increasingly being applied in biomedical fields. This is ascribed to their good physiochemical properties, unique structure and high biological compatibility. However, the osteogenic activity and suitability of these materials for bone tissue engineering are not clearly understood. Thus, the aim of this study is to evaluate the biocompatibility, osteoinductivity and guided bone regeneration ability of Ti3C2Tx MXene in vitro and in vivo.Methods: Multilayered Ti3C2Tx MXene films were prepared and characterized by XRD and SEM. In vitro experiments were performed to evaluate the effect of MXene films on cell adhesion and morphology with SEM and fluorescence microscopy. The cytotoxicity of MXene films was detected with the Live/Dead double-staining tests. The EdU assay was employed to evaluate cell proliferation on MXene films and ALP activity was tested to determine the effect of the films on osteogenic differentiation in vitro. The mRNA expression of osteogenic differentiation-related markers was measured using qRT-PCR. In vivo animal studies were performed in which the MXene films were implanted subcutaneously in rats to evaluate biocompatibility and host tissue response in vivo. In addition, a rat calvarial defect model was established to examine the bone regeneration performance of the Ti3C2Tx MXene films. The specimens were analyzed with micro-CT evaluation and histological tests.Results: The XRD and SEM analyses revealed that the Ti3C2Tx MXene film was successfully synthesized. The cellular experiments showed that MXene films were highly cytocompatible and enhanced osteogenic differentiation in vitro. When implanted into subcutaneous sites and calvarial defect sites in rats, MXene films showed good biocompatibility, osteoinductivity and bone regeneration activity in vivo.Conclusion: In summary, this study presents new applications of MXenes in bone tissue engineering and in guided bone regeneration therapy.Keywords: Ti3C2Tx, bone tissue engineering, GBR, osteoinductivity, biocompatibilityZhang JFu YMo ADove Medical Pressarticleti3c2txbone tissue engineeringgbrosteoinductivitybiocompatibilityMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 10091-10103 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	ti3c2tx bone tissue engineering gbr osteoinductivity biocompatibility Medicine (General) R5-920
spellingShingle	ti3c2tx bone tissue engineering gbr osteoinductivity biocompatibility Medicine (General) R5-920 Zhang J Fu Y Mo A Multilayered Titanium Carbide MXene Film for Guided Bone Regeneration
description	Jiebing Zhang, Yu Fu, Anchun Mo State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, People’s Republic of ChinaCorrespondence: Anchun MoState Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, People’s Republic of ChinaEmail moanchun@163.comPurpose: MXenes are two-dimensional (2D) materials that are increasingly being applied in biomedical fields. This is ascribed to their good physiochemical properties, unique structure and high biological compatibility. However, the osteogenic activity and suitability of these materials for bone tissue engineering are not clearly understood. Thus, the aim of this study is to evaluate the biocompatibility, osteoinductivity and guided bone regeneration ability of Ti3C2Tx MXene in vitro and in vivo.Methods: Multilayered Ti3C2Tx MXene films were prepared and characterized by XRD and SEM. In vitro experiments were performed to evaluate the effect of MXene films on cell adhesion and morphology with SEM and fluorescence microscopy. The cytotoxicity of MXene films was detected with the Live/Dead double-staining tests. The EdU assay was employed to evaluate cell proliferation on MXene films and ALP activity was tested to determine the effect of the films on osteogenic differentiation in vitro. The mRNA expression of osteogenic differentiation-related markers was measured using qRT-PCR. In vivo animal studies were performed in which the MXene films were implanted subcutaneously in rats to evaluate biocompatibility and host tissue response in vivo. In addition, a rat calvarial defect model was established to examine the bone regeneration performance of the Ti3C2Tx MXene films. The specimens were analyzed with micro-CT evaluation and histological tests.Results: The XRD and SEM analyses revealed that the Ti3C2Tx MXene film was successfully synthesized. The cellular experiments showed that MXene films were highly cytocompatible and enhanced osteogenic differentiation in vitro. When implanted into subcutaneous sites and calvarial defect sites in rats, MXene films showed good biocompatibility, osteoinductivity and bone regeneration activity in vivo.Conclusion: In summary, this study presents new applications of MXenes in bone tissue engineering and in guided bone regeneration therapy.Keywords: Ti3C2Tx, bone tissue engineering, GBR, osteoinductivity, biocompatibility
format	article
author	Zhang J Fu Y Mo A
author_facet	Zhang J Fu Y Mo A
author_sort	Zhang J
title	Multilayered Titanium Carbide MXene Film for Guided Bone Regeneration
title_short	Multilayered Titanium Carbide MXene Film for Guided Bone Regeneration
title_full	Multilayered Titanium Carbide MXene Film for Guided Bone Regeneration
title_fullStr	Multilayered Titanium Carbide MXene Film for Guided Bone Regeneration
title_full_unstemmed	Multilayered Titanium Carbide MXene Film for Guided Bone Regeneration
title_sort	multilayered titanium carbide mxene film for guided bone regeneration
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/c0dae6b216fd44e9977696c153d77237
work_keys_str_mv	AT zhangj multilayeredtitaniumcarbidemxenefilmforguidedboneregeneration AT fuy multilayeredtitaniumcarbidemxenefilmforguidedboneregeneration AT moa multilayeredtitaniumcarbidemxenefilmforguidedboneregeneration
_version_	1718398268982951936

Multilayered Titanium Carbide MXene Film for Guided Bone Regeneration

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