RNA-seq profiles of immune related genes in the staghorn coral Acropora cervicornis infected with white band disease.

Coral diseases are among the most serious threats to coral reefs worldwide, yet most coral diseases remain poorly understood. How the coral host responds to pathogen infection is an area where very little is known. Here we used next-generation RNA-sequencing (RNA-seq) to produce a transcriptome-wide...

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Autores principales:	Silvia Libro, Stefan T Kaluziak, Steven V Vollmer
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c0ddb1f5aa73448388aef4c9cd9dc9be
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spelling	oai:doaj.org-article:c0ddb1f5aa73448388aef4c9cd9dc9be2021-11-18T08:45:07ZRNA-seq profiles of immune related genes in the staghorn coral Acropora cervicornis infected with white band disease.1932-620310.1371/journal.pone.0081821https://doaj.org/article/c0ddb1f5aa73448388aef4c9cd9dc9be2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24278460/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Coral diseases are among the most serious threats to coral reefs worldwide, yet most coral diseases remain poorly understood. How the coral host responds to pathogen infection is an area where very little is known. Here we used next-generation RNA-sequencing (RNA-seq) to produce a transcriptome-wide profile of the immune response of the Staghorn coral Acropora cervicornis to White Band Disease (WBD) by comparing infected versus healthy (asymptomatic) coral tissues. The transcriptome of A. cervicornis was assembled de novo from A-tail selected Illumina mRNA-seq data from whole coral tissues, and parsed bioinformatically into coral and non-coral transcripts using existing Acropora genomes in order to identify putative coral transcripts. Differentially expressed transcripts were identified in the coral and non-coral datasets to identify genes that were up- and down-regulated due to disease infection. RNA-seq analyses indicate that infected corals exhibited significant changes in gene expression across 4% (1,805 out of 47,748 transcripts) of the coral transcriptome. The primary response to infection included transcripts involved in macrophage-mediated pathogen recognition and ROS production, two hallmarks of phagocytosis, as well as key mediators of apoptosis and calcium homeostasis. The strong up-regulation of the enzyme allene oxide synthase-lipoxygenase suggests a key role of the allene oxide pathway in coral immunity. Interestingly, none of the three primary innate immune pathways--Toll-like receptors (TLR), Complement, and prophenoloxydase pathways, were strongly associated with the response of A. cervicornis to infection. Five-hundred and fifty differentially expressed non-coral transcripts were classified as metazoan (n = 84), algal or plant (n = 52), fungi (n = 24) and protozoans (n = 13). None of the 52 putative Symbiodinium or algal transcript had any clear immune functions indicating that the immune response is driven by the coral host, and not its algal symbionts.Silvia LibroStefan T KaluziakSteven V VollmerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e81821 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Silvia Libro Stefan T Kaluziak Steven V Vollmer RNA-seq profiles of immune related genes in the staghorn coral Acropora cervicornis infected with white band disease.
description	Coral diseases are among the most serious threats to coral reefs worldwide, yet most coral diseases remain poorly understood. How the coral host responds to pathogen infection is an area where very little is known. Here we used next-generation RNA-sequencing (RNA-seq) to produce a transcriptome-wide profile of the immune response of the Staghorn coral Acropora cervicornis to White Band Disease (WBD) by comparing infected versus healthy (asymptomatic) coral tissues. The transcriptome of A. cervicornis was assembled de novo from A-tail selected Illumina mRNA-seq data from whole coral tissues, and parsed bioinformatically into coral and non-coral transcripts using existing Acropora genomes in order to identify putative coral transcripts. Differentially expressed transcripts were identified in the coral and non-coral datasets to identify genes that were up- and down-regulated due to disease infection. RNA-seq analyses indicate that infected corals exhibited significant changes in gene expression across 4% (1,805 out of 47,748 transcripts) of the coral transcriptome. The primary response to infection included transcripts involved in macrophage-mediated pathogen recognition and ROS production, two hallmarks of phagocytosis, as well as key mediators of apoptosis and calcium homeostasis. The strong up-regulation of the enzyme allene oxide synthase-lipoxygenase suggests a key role of the allene oxide pathway in coral immunity. Interestingly, none of the three primary innate immune pathways--Toll-like receptors (TLR), Complement, and prophenoloxydase pathways, were strongly associated with the response of A. cervicornis to infection. Five-hundred and fifty differentially expressed non-coral transcripts were classified as metazoan (n = 84), algal or plant (n = 52), fungi (n = 24) and protozoans (n = 13). None of the 52 putative Symbiodinium or algal transcript had any clear immune functions indicating that the immune response is driven by the coral host, and not its algal symbionts.
format	article
author	Silvia Libro Stefan T Kaluziak Steven V Vollmer
author_facet	Silvia Libro Stefan T Kaluziak Steven V Vollmer
author_sort	Silvia Libro
title	RNA-seq profiles of immune related genes in the staghorn coral Acropora cervicornis infected with white band disease.
title_short	RNA-seq profiles of immune related genes in the staghorn coral Acropora cervicornis infected with white band disease.
title_full	RNA-seq profiles of immune related genes in the staghorn coral Acropora cervicornis infected with white band disease.
title_fullStr	RNA-seq profiles of immune related genes in the staghorn coral Acropora cervicornis infected with white band disease.
title_full_unstemmed	RNA-seq profiles of immune related genes in the staghorn coral Acropora cervicornis infected with white band disease.
title_sort	rna-seq profiles of immune related genes in the staghorn coral acropora cervicornis infected with white band disease.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/c0ddb1f5aa73448388aef4c9cd9dc9be
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RNA-seq profiles of immune related genes in the staghorn coral Acropora cervicornis infected with white band disease.

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