Protective Effect of <i>Siegesbeckia orientalis</i> on Pancreatic β-Cells under High Glucose-Induced Glucotoxicity

The glucotoxicity caused by long-term exposure of β-cells to high glucose (HG) conditions may lead to the generation of more reactive oxygen species (ROS), reduce the activity of antioxidant enzymes, cause cell damage and apoptosis, and induce insulin secretion dysfunction. <i>Siegesbeckia ori...

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Autores principales:	Chi-Chang Chang, Jer-Yiing Houng, Shih-Wei Wang, Chin-Feng Hsuan, Yung-Chuan Lu, Tzu-Hsien Chang, Ya-Ling Chen
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Siegesbeckia orientalis</i> pancreatic β-cells glucotoxicity oxidative stress cell proliferation antioxidant enzyme activity Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/c0e3920f7b6f444695973db35132613f
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spelling	oai:doaj.org-article:c0e3920f7b6f444695973db35132613f2021-11-25T16:41:52ZProtective Effect of <i>Siegesbeckia orientalis</i> on Pancreatic β-Cells under High Glucose-Induced Glucotoxicity10.3390/app1122109632076-3417https://doaj.org/article/c0e3920f7b6f444695973db35132613f2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-3417/11/22/10963https://doaj.org/toc/2076-3417The glucotoxicity caused by long-term exposure of β-cells to high glucose (HG) conditions may lead to the generation of more reactive oxygen species (ROS), reduce the activity of antioxidant enzymes, cause cell damage and apoptosis, and induce insulin secretion dysfunction. <i>Siegesbeckia orientalis</i> linne is a traditional folk herbal medicine used to treat snake bites, rheumatoid arthritis, allergies, and immune deficiencies. In this study, we evaluated the protective effect of <i>S. orientalis</i> ethanol extract (SOE) on cell death and oxidative stress in RIN-m5f pancreatic β-cells stimulated by two HG concentrations (50–100 mM). In the cell viability assay, SOE could significantly increase the survival rate of pancreatic β-cells under HG-induced conditions. For the oxidative stress induced by HG condition, the treatment of SOE effectively reduced the ROS formation, increased the content of intracellular glutathione, and up-regulated the expression of antioxidant enzymes, catalase, superoxide dismutase, and glutathione peroxidase. As a result, the SOE treatment could decrease the glucotoxicity-mediated oxidative damage on RIN-m5F β-cells. Moreover, SOE had the function of regulating insulin secretion in pancreatic β-cells under different HG-mediated conditions. It could decrease the increasing intracellular insulin secretion under the low glucose concentration to normal level; while increase the decreasing intracellular insulin secretion under the relatively high glucose concentration to normal level. Taken together, this study suggests that SOE has a protective effect on pancreatic β-cells under the HG-stimulated glucotoxic environment.Chi-Chang ChangJer-Yiing HoungShih-Wei WangChin-Feng HsuanYung-Chuan LuTzu-Hsien ChangYa-Ling ChenMDPI AGarticle<i>Siegesbeckia orientalis</i>pancreatic β-cellsglucotoxicityoxidative stresscell proliferationantioxidant enzyme activityTechnologyTEngineering (General). Civil engineering (General)TA1-2040Biology (General)QH301-705.5PhysicsQC1-999ChemistryQD1-999ENApplied Sciences, Vol 11, Iss 10963, p 10963 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Siegesbeckia orientalis</i> pancreatic β-cells glucotoxicity oxidative stress cell proliferation antioxidant enzyme activity Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
spellingShingle	<i>Siegesbeckia orientalis</i> pancreatic β-cells glucotoxicity oxidative stress cell proliferation antioxidant enzyme activity Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999 Chi-Chang Chang Jer-Yiing Houng Shih-Wei Wang Chin-Feng Hsuan Yung-Chuan Lu Tzu-Hsien Chang Ya-Ling Chen Protective Effect of <i>Siegesbeckia orientalis</i> on Pancreatic β-Cells under High Glucose-Induced Glucotoxicity
description	The glucotoxicity caused by long-term exposure of β-cells to high glucose (HG) conditions may lead to the generation of more reactive oxygen species (ROS), reduce the activity of antioxidant enzymes, cause cell damage and apoptosis, and induce insulin secretion dysfunction. <i>Siegesbeckia orientalis</i> linne is a traditional folk herbal medicine used to treat snake bites, rheumatoid arthritis, allergies, and immune deficiencies. In this study, we evaluated the protective effect of <i>S. orientalis</i> ethanol extract (SOE) on cell death and oxidative stress in RIN-m5f pancreatic β-cells stimulated by two HG concentrations (50–100 mM). In the cell viability assay, SOE could significantly increase the survival rate of pancreatic β-cells under HG-induced conditions. For the oxidative stress induced by HG condition, the treatment of SOE effectively reduced the ROS formation, increased the content of intracellular glutathione, and up-regulated the expression of antioxidant enzymes, catalase, superoxide dismutase, and glutathione peroxidase. As a result, the SOE treatment could decrease the glucotoxicity-mediated oxidative damage on RIN-m5F β-cells. Moreover, SOE had the function of regulating insulin secretion in pancreatic β-cells under different HG-mediated conditions. It could decrease the increasing intracellular insulin secretion under the low glucose concentration to normal level; while increase the decreasing intracellular insulin secretion under the relatively high glucose concentration to normal level. Taken together, this study suggests that SOE has a protective effect on pancreatic β-cells under the HG-stimulated glucotoxic environment.
format	article
author	Chi-Chang Chang Jer-Yiing Houng Shih-Wei Wang Chin-Feng Hsuan Yung-Chuan Lu Tzu-Hsien Chang Ya-Ling Chen
author_facet	Chi-Chang Chang Jer-Yiing Houng Shih-Wei Wang Chin-Feng Hsuan Yung-Chuan Lu Tzu-Hsien Chang Ya-Ling Chen
author_sort	Chi-Chang Chang
title	Protective Effect of <i>Siegesbeckia orientalis</i> on Pancreatic β-Cells under High Glucose-Induced Glucotoxicity
title_short	Protective Effect of <i>Siegesbeckia orientalis</i> on Pancreatic β-Cells under High Glucose-Induced Glucotoxicity
title_full	Protective Effect of <i>Siegesbeckia orientalis</i> on Pancreatic β-Cells under High Glucose-Induced Glucotoxicity
title_fullStr	Protective Effect of <i>Siegesbeckia orientalis</i> on Pancreatic β-Cells under High Glucose-Induced Glucotoxicity
title_full_unstemmed	Protective Effect of <i>Siegesbeckia orientalis</i> on Pancreatic β-Cells under High Glucose-Induced Glucotoxicity
title_sort	protective effect of <i>siegesbeckia orientalis</i> on pancreatic β-cells under high glucose-induced glucotoxicity
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/c0e3920f7b6f444695973db35132613f
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Protective Effect of <i>Siegesbeckia orientalis</i> on Pancreatic β-Cells under High Glucose-Induced Glucotoxicity

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