Curcuminoid-loaded poly(methyl methacrylate) nanoparticles for cancer therapy

Abhispa Sahu,1 Pratima Solanki,2 Susmita Mitra1 1Amity Institute of Nanotechnology, Amity University, Noida, 2Special Centre for Nanosciences, Jawaharlal Nehru University, New Delhi, India Abstract: Curcuminoids (Curs), oleoresins from Curcuma longa L., have known anticarcinogenic and anti-inflamm...

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Autores principales:	Sahu A, Solanki P, Mitra S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	polymeric nanoparticle squamous cell carcinoma A549 cell line anticarcinogenic Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/c0e5294ba3fc4b37928faee7299ff0ca
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spelling	oai:doaj.org-article:c0e5294ba3fc4b37928faee7299ff0ca2021-12-02T04:51:13ZCurcuminoid-loaded poly(methyl methacrylate) nanoparticles for cancer therapy1178-2013https://doaj.org/article/c0e5294ba3fc4b37928faee7299ff0ca2018-03-01T00:00:00Zhttps://www.dovepress.com/curcuminoid-loaded--polymethyl-methacrylate-nanoparticles-for-cancer-t-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Abhispa Sahu,1 Pratima Solanki,2 Susmita Mitra1 1Amity Institute of Nanotechnology, Amity University, Noida, 2Special Centre for Nanosciences, Jawaharlal Nehru University, New Delhi, India Abstract: Curcuminoids (Curs), oleoresins from Curcuma longa L., have known anticarcinogenic and anti-inflammatory properties, but high toxicity, poor aqueous solubility and susceptibility to degradation in body fluids are deterrents to their clinical administration. Poly(methyl methacrylate) nanoparticles (PMMA-NPs) are biocompatible and resilient and can entrap hydrophobic drugs. The present investigation is related to solubilizing Curs by incorporating them in these nanoparticles (NPs) and is related to a study comparing the anticarcinogenic effect of drug-loaded NPs with free Cur using lung cancer (A549) cell line. Freshly extracted oleoresins were post loaded in PMMA-NPs prepared using emulsion polymerization. The presence of the three components of oleoresins was confirmed by thin-layer chromatography. The size and morphology of void and loaded NPs were determined by dynamic light scattering, scanning electron microscopy and transmission electron microscopy. The NPs were spherical with diameters of 192.66±5 nm (void) and 199.16±5 nm (loaded). Drug loading and encapsulation efficiency were 6% and 93%, respectively. From the Fourier transform infrared spectroscopy spectra, the characteristic absorption vibration of poly(methyl methacrylate) and the bands at 1,383, 1,233 and 962 cm−1 for Cur moiety were observed. Drug release up to 10 days was estimated in buffer, saline and serum. The highest release of ~55% in ~3 days was noted in buffer that exhibited the highest bioavailability. The in vitro anticancer activity of loaded drug was evaluated up to 72 hours by MTT assay using A549 cell line. Cellular uptake of dye-loaded NPs was visualized within 30 minutes of incubation. The results revealed that the dose- and time-dependent cell death in case of loaded PMMA-NPs was comparable to that of free Cur. According to the study, the drug-loaded PMMA-NPs appear to be highly suitable for effective, localized and safe chemotherapy. Keywords: polymeric nanoparticle, squamous cell carcinoma, A549 cell line, anticarcinogenicSahu ASolanki PMitra SDove Medical Pressarticlepolymeric nanoparticlesquamous cell carcinomaA549 cell lineanticarcinogenicMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 101-105 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	polymeric nanoparticle squamous cell carcinoma A549 cell line anticarcinogenic Medicine (General) R5-920
spellingShingle	polymeric nanoparticle squamous cell carcinoma A549 cell line anticarcinogenic Medicine (General) R5-920 Sahu A Solanki P Mitra S Curcuminoid-loaded poly(methyl methacrylate) nanoparticles for cancer therapy
description	Abhispa Sahu,1 Pratima Solanki,2 Susmita Mitra1 1Amity Institute of Nanotechnology, Amity University, Noida, 2Special Centre for Nanosciences, Jawaharlal Nehru University, New Delhi, India Abstract: Curcuminoids (Curs), oleoresins from Curcuma longa L., have known anticarcinogenic and anti-inflammatory properties, but high toxicity, poor aqueous solubility and susceptibility to degradation in body fluids are deterrents to their clinical administration. Poly(methyl methacrylate) nanoparticles (PMMA-NPs) are biocompatible and resilient and can entrap hydrophobic drugs. The present investigation is related to solubilizing Curs by incorporating them in these nanoparticles (NPs) and is related to a study comparing the anticarcinogenic effect of drug-loaded NPs with free Cur using lung cancer (A549) cell line. Freshly extracted oleoresins were post loaded in PMMA-NPs prepared using emulsion polymerization. The presence of the three components of oleoresins was confirmed by thin-layer chromatography. The size and morphology of void and loaded NPs were determined by dynamic light scattering, scanning electron microscopy and transmission electron microscopy. The NPs were spherical with diameters of 192.66±5 nm (void) and 199.16±5 nm (loaded). Drug loading and encapsulation efficiency were 6% and 93%, respectively. From the Fourier transform infrared spectroscopy spectra, the characteristic absorption vibration of poly(methyl methacrylate) and the bands at 1,383, 1,233 and 962 cm−1 for Cur moiety were observed. Drug release up to 10 days was estimated in buffer, saline and serum. The highest release of ~55% in ~3 days was noted in buffer that exhibited the highest bioavailability. The in vitro anticancer activity of loaded drug was evaluated up to 72 hours by MTT assay using A549 cell line. Cellular uptake of dye-loaded NPs was visualized within 30 minutes of incubation. The results revealed that the dose- and time-dependent cell death in case of loaded PMMA-NPs was comparable to that of free Cur. According to the study, the drug-loaded PMMA-NPs appear to be highly suitable for effective, localized and safe chemotherapy. Keywords: polymeric nanoparticle, squamous cell carcinoma, A549 cell line, anticarcinogenic
format	article
author	Sahu A Solanki P Mitra S
author_facet	Sahu A Solanki P Mitra S
author_sort	Sahu A
title	Curcuminoid-loaded poly(methyl methacrylate) nanoparticles for cancer therapy
title_short	Curcuminoid-loaded poly(methyl methacrylate) nanoparticles for cancer therapy
title_full	Curcuminoid-loaded poly(methyl methacrylate) nanoparticles for cancer therapy
title_fullStr	Curcuminoid-loaded poly(methyl methacrylate) nanoparticles for cancer therapy
title_full_unstemmed	Curcuminoid-loaded poly(methyl methacrylate) nanoparticles for cancer therapy
title_sort	curcuminoid-loaded poly(methyl methacrylate) nanoparticles for cancer therapy
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/c0e5294ba3fc4b37928faee7299ff0ca
work_keys_str_mv	AT sahua curcuminoidloadedpolymethylmethacrylatenanoparticlesforcancertherapy AT solankip curcuminoidloadedpolymethylmethacrylatenanoparticlesforcancertherapy AT mitras curcuminoidloadedpolymethylmethacrylatenanoparticlesforcancertherapy
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Curcuminoid-loaded poly(methyl methacrylate) nanoparticles for cancer therapy

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