Study of Some Virulence Genes of Enterococci Species Isolated from Raw Milk and Some Milk Products

A total of 150 random samples of raw cow milk and some locally manufactured dairy products including yogurt, Kareish cheese, and ice cream were collected from various dairy shops, and supermarkets in Qena city, Egypt. Samples were examined for the presence of Enterococcus spp. The investigation reve...

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Autor principal:	Margret Yousry Shafeek
Formato:	article
Lenguaje:	EN
Publicado:	South Valley University 2018
Materias:	Enterococcus spp Milk Dairy products PCR Virulence Genes Agriculture S Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/c0e694abf317443a909ae39564e3c397
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spelling	oai:doaj.org-article:c0e694abf317443a909ae39564e3c3972021-12-02T00:44:46ZStudy of Some Virulence Genes of Enterococci Species Isolated from Raw Milk and Some Milk Products2535-18262535-1877https://doaj.org/article/c0e694abf317443a909ae39564e3c3972018-06-01T00:00:00Zhttps://portal.svu.edu.eg/svu-src/index.php/ijvs/article/view/18https://doaj.org/toc/2535-1826https://doaj.org/toc/2535-1877A total of 150 random samples of raw cow milk and some locally manufactured dairy products including yogurt, Kareish cheese, and ice cream were collected from various dairy shops, and supermarkets in Qena city, Egypt. Samples were examined for the presence of Enterococcus spp. The investigation revealed that 64, 28, 76, 72 and 16 % of the examined raw milk samples, large and small-scale yogurt, Kareish cheese and ice cream were contaminated with Enterococcus spp., respectively. Isolates were identified as E. faecalis and E. faecium in percentages of (8 & 32), (16 & 0), (8 & 28), (8 & 36), and (4 & 0) in the examined raw milk samples, large and small-scale yoghurt, Kareish cheese and ice cream, respectively. The obtained isolates were screened for presence of some virulence genes gelE, asa1, esp and cylA using multiplex PCR. The results indicated that gelE, asa1, esp and cylA were located in 53.9, 76.9, 69.2, and 30.8% of the total E. faecalis isolates and in 46.9, 71.9, 53.1, and 34.3 % of the total E. faecium isolates, respectively. The asa1 and esp genes were the predominant virulence traits among all investigated enterococci isolates followed by gelE and cylA genes. Therefore, the results of this study showed that milk and dairy products can play an important role in the spread of Enterococci with virulence potential through the food chain to the human population.Margret Yousry ShafeekSouth Valley UniversityarticleEnterococcus sppMilkDairy productsPCRVirulence GenesAgricultureSVeterinary medicineSF600-1100ENSVU-International Journal of Veterinary Sciences, Vol 1, Iss 1, Pp 102-113 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Enterococcus spp Milk Dairy products PCR Virulence Genes Agriculture S Veterinary medicine SF600-1100
spellingShingle	Enterococcus spp Milk Dairy products PCR Virulence Genes Agriculture S Veterinary medicine SF600-1100 Margret Yousry Shafeek Study of Some Virulence Genes of Enterococci Species Isolated from Raw Milk and Some Milk Products
description	A total of 150 random samples of raw cow milk and some locally manufactured dairy products including yogurt, Kareish cheese, and ice cream were collected from various dairy shops, and supermarkets in Qena city, Egypt. Samples were examined for the presence of Enterococcus spp. The investigation revealed that 64, 28, 76, 72 and 16 % of the examined raw milk samples, large and small-scale yogurt, Kareish cheese and ice cream were contaminated with Enterococcus spp., respectively. Isolates were identified as E. faecalis and E. faecium in percentages of (8 & 32), (16 & 0), (8 & 28), (8 & 36), and (4 & 0) in the examined raw milk samples, large and small-scale yoghurt, Kareish cheese and ice cream, respectively. The obtained isolates were screened for presence of some virulence genes gelE, asa1, esp and cylA using multiplex PCR. The results indicated that gelE, asa1, esp and cylA were located in 53.9, 76.9, 69.2, and 30.8% of the total E. faecalis isolates and in 46.9, 71.9, 53.1, and 34.3 % of the total E. faecium isolates, respectively. The asa1 and esp genes were the predominant virulence traits among all investigated enterococci isolates followed by gelE and cylA genes. Therefore, the results of this study showed that milk and dairy products can play an important role in the spread of Enterococci with virulence potential through the food chain to the human population.
format	article
author	Margret Yousry Shafeek
author_facet	Margret Yousry Shafeek
author_sort	Margret Yousry Shafeek
title	Study of Some Virulence Genes of Enterococci Species Isolated from Raw Milk and Some Milk Products
title_short	Study of Some Virulence Genes of Enterococci Species Isolated from Raw Milk and Some Milk Products
title_full	Study of Some Virulence Genes of Enterococci Species Isolated from Raw Milk and Some Milk Products
title_fullStr	Study of Some Virulence Genes of Enterococci Species Isolated from Raw Milk and Some Milk Products
title_full_unstemmed	Study of Some Virulence Genes of Enterococci Species Isolated from Raw Milk and Some Milk Products
title_sort	study of some virulence genes of enterococci species isolated from raw milk and some milk products
publisher	South Valley University
publishDate	2018
url	https://doaj.org/article/c0e694abf317443a909ae39564e3c397
work_keys_str_mv	AT margretyousryshafeek studyofsomevirulencegenesofenterococcispeciesisolatedfromrawmilkandsomemilkproducts
_version_	1718403522753462272

Study of Some Virulence Genes of Enterococci Species Isolated from Raw Milk and Some Milk Products

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