The Role of the Notch Signal Pathway in Mucosal Cell Metaplasia in Mouse Acute Otitis Media
Abstract Otitis media (OM) is a major cause of morbidity in pediatric and adult patients. This inflammatory condition is characterized by mucous cell hyperplasia that is thought to produce mucins from the middle ear mucosa. We are interested in the role of Notch signalling pathway in this inflammato...
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2017
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oai:doaj.org-article:c0eb372a4ce94c559ac0370d4f9b8f8a2021-12-02T12:32:05ZThe Role of the Notch Signal Pathway in Mucosal Cell Metaplasia in Mouse Acute Otitis Media10.1038/s41598-017-04639-z2045-2322https://doaj.org/article/c0eb372a4ce94c559ac0370d4f9b8f8a2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04639-zhttps://doaj.org/toc/2045-2322Abstract Otitis media (OM) is a major cause of morbidity in pediatric and adult patients. This inflammatory condition is characterized by mucous cell hyperplasia that is thought to produce mucins from the middle ear mucosa. We are interested in the role of Notch signalling pathway in this inflammatory process. Using an acute otitis media (AOM) mouse model through injection of Streptococcus Pneumoniae into the middle ear, histopathologic examination and quantitative RT-PCR, acute inflammation with the thickness of mucosa, Goblet cell hyperplasia, and cilia loss were determined and gene expression related to the Notch signaling pathway were evaluated. Upregulation of the mucous cell markers, Argr2 and Muc5AC, and downregulation of the cilia cell marker, Foxj1 and Dnai2, were observed in AOM. In addition, genes encoding Notch receptors and ligands (Notch1, Notch2, Notch3, Notch4 and Dll1) and the Notch target genes (Hes1, Hes5, Hey1, NRARP) in AOM decreased significantly. The expression of the Notch1 and Jagged1 also showed down-regulation throughout the mouse middle ear epithelium. Taken together, this study suggests that downregulation of the Notch signaling pathway is involved in the mucosa hyperplasia during AOM.Xiang LiuNing CongXiang ChengRui MaJing WangYi-bo HuangMeng ZhaoXin-wei WangFang-Lu ChiDong-Dong RenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017) |
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Medicine R Science Q Xiang Liu Ning Cong Xiang Cheng Rui Ma Jing Wang Yi-bo Huang Meng Zhao Xin-wei Wang Fang-Lu Chi Dong-Dong Ren The Role of the Notch Signal Pathway in Mucosal Cell Metaplasia in Mouse Acute Otitis Media |
| description |
Abstract Otitis media (OM) is a major cause of morbidity in pediatric and adult patients. This inflammatory condition is characterized by mucous cell hyperplasia that is thought to produce mucins from the middle ear mucosa. We are interested in the role of Notch signalling pathway in this inflammatory process. Using an acute otitis media (AOM) mouse model through injection of Streptococcus Pneumoniae into the middle ear, histopathologic examination and quantitative RT-PCR, acute inflammation with the thickness of mucosa, Goblet cell hyperplasia, and cilia loss were determined and gene expression related to the Notch signaling pathway were evaluated. Upregulation of the mucous cell markers, Argr2 and Muc5AC, and downregulation of the cilia cell marker, Foxj1 and Dnai2, were observed in AOM. In addition, genes encoding Notch receptors and ligands (Notch1, Notch2, Notch3, Notch4 and Dll1) and the Notch target genes (Hes1, Hes5, Hey1, NRARP) in AOM decreased significantly. The expression of the Notch1 and Jagged1 also showed down-regulation throughout the mouse middle ear epithelium. Taken together, this study suggests that downregulation of the Notch signaling pathway is involved in the mucosa hyperplasia during AOM. |
| format |
article |
| author |
Xiang Liu Ning Cong Xiang Cheng Rui Ma Jing Wang Yi-bo Huang Meng Zhao Xin-wei Wang Fang-Lu Chi Dong-Dong Ren |
| author_facet |
Xiang Liu Ning Cong Xiang Cheng Rui Ma Jing Wang Yi-bo Huang Meng Zhao Xin-wei Wang Fang-Lu Chi Dong-Dong Ren |
| author_sort |
Xiang Liu |
| title |
The Role of the Notch Signal Pathway in Mucosal Cell Metaplasia in Mouse Acute Otitis Media |
| title_short |
The Role of the Notch Signal Pathway in Mucosal Cell Metaplasia in Mouse Acute Otitis Media |
| title_full |
The Role of the Notch Signal Pathway in Mucosal Cell Metaplasia in Mouse Acute Otitis Media |
| title_fullStr |
The Role of the Notch Signal Pathway in Mucosal Cell Metaplasia in Mouse Acute Otitis Media |
| title_full_unstemmed |
The Role of the Notch Signal Pathway in Mucosal Cell Metaplasia in Mouse Acute Otitis Media |
| title_sort |
role of the notch signal pathway in mucosal cell metaplasia in mouse acute otitis media |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/c0eb372a4ce94c559ac0370d4f9b8f8a |
| work_keys_str_mv |
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