Phenotypic characterization of NKT-like cells and evaluation of specifically related cytokines for the prediction of unexplained recurrent miscarriage

Problem: Immune system dysregulation is a major cause of unexplained recurrent miscarriage (URM). Women with URM need screening for their pregnancy microenvironment and immune regulators, to prevent spontaneous abortion. Method of study: In this study we evaluated NKT-like cell subsets in peripheral...

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Autores principales:	Wafaa S. Khalaf, Mohammad R.A. Mahmoud, Walid F. Elkhatib, Hany R. Hashem, Wafaa E. Soliman
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	NKT-like cells Cytokines Prediction Unexplained recurrent miscarriage Science (General) Q1-390 Social sciences (General) H1-99
Acceso en línea:	https://doaj.org/article/c0eb57d4a62644578221a51067282bbd
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spelling	oai:doaj.org-article:c0eb57d4a62644578221a51067282bbd2021-12-02T05:03:05ZPhenotypic characterization of NKT-like cells and evaluation of specifically related cytokines for the prediction of unexplained recurrent miscarriage2405-844010.1016/j.heliyon.2021.e08409https://doaj.org/article/c0eb57d4a62644578221a51067282bbd2021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2405844021025123https://doaj.org/toc/2405-8440Problem: Immune system dysregulation is a major cause of unexplained recurrent miscarriage (URM). Women with URM need screening for their pregnancy microenvironment and immune regulators, to prevent spontaneous abortion. Method of study: In this study we evaluated NKT-like cell subsets in peripheral venous blood of women with URM using flow cytometry. The expression levels of specifically related Th1 cytokines (IFN-γ and IL-2), Th2 cytokine (IL-4), and Th17 cytokines (IL-17), were measured using enzyme-linked immunosorbent assay. Results: The percentage of CD16+CD56+NKT-like (Double Positive NKT-like; DPNKT-like) cell subset, and the levels of IL-2 and IFN-γ were significantly elevated in blood of non-pregnant and pregnant patients with URM compared with the healthy control groups, and these parameters were significantly increased after pregnancy in the same patients with URM. Based on the prevalence of the candidate immunological factors in patients with URM, the prognostic significance of the NKT-like cell subsets, IFN-γ and IL-2 profiles were evaluated as potential predictors of URM. A cut-off point of 2.55% for DPNKT-like cell subset in the blood and cut-off values of 39.5 and 20.5 pg/ml for the levels of IFN-γ and IL-2, respectively could be used for the prediction of the risk of spontaneous abortion. To the best of our knowledge, this is the first study that described the prognostic significance of the aforementioned immunological parameters before and after pregnancy, and highlighted the correlation of NKT-like cells and the candidate Th1 cytokines with pregnancy loss in women with URM. Conclusions: DPNKT-like cells, IFN-γ and IL-2 patient profiles could be used as markers to predict the risk of miscarriage in patients with URM.Wafaa S. KhalafMohammad R.A. MahmoudWalid F. ElkhatibHany R. HashemWafaa E. SolimanElsevierarticleNKT-like cellsCytokinesPredictionUnexplained recurrent miscarriageScience (General)Q1-390Social sciences (General)H1-99ENHeliyon, Vol 7, Iss 11, Pp e08409- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	NKT-like cells Cytokines Prediction Unexplained recurrent miscarriage Science (General) Q1-390 Social sciences (General) H1-99
spellingShingle	NKT-like cells Cytokines Prediction Unexplained recurrent miscarriage Science (General) Q1-390 Social sciences (General) H1-99 Wafaa S. Khalaf Mohammad R.A. Mahmoud Walid F. Elkhatib Hany R. Hashem Wafaa E. Soliman Phenotypic characterization of NKT-like cells and evaluation of specifically related cytokines for the prediction of unexplained recurrent miscarriage
description	Problem: Immune system dysregulation is a major cause of unexplained recurrent miscarriage (URM). Women with URM need screening for their pregnancy microenvironment and immune regulators, to prevent spontaneous abortion. Method of study: In this study we evaluated NKT-like cell subsets in peripheral venous blood of women with URM using flow cytometry. The expression levels of specifically related Th1 cytokines (IFN-γ and IL-2), Th2 cytokine (IL-4), and Th17 cytokines (IL-17), were measured using enzyme-linked immunosorbent assay. Results: The percentage of CD16+CD56+NKT-like (Double Positive NKT-like; DPNKT-like) cell subset, and the levels of IL-2 and IFN-γ were significantly elevated in blood of non-pregnant and pregnant patients with URM compared with the healthy control groups, and these parameters were significantly increased after pregnancy in the same patients with URM. Based on the prevalence of the candidate immunological factors in patients with URM, the prognostic significance of the NKT-like cell subsets, IFN-γ and IL-2 profiles were evaluated as potential predictors of URM. A cut-off point of 2.55% for DPNKT-like cell subset in the blood and cut-off values of 39.5 and 20.5 pg/ml for the levels of IFN-γ and IL-2, respectively could be used for the prediction of the risk of spontaneous abortion. To the best of our knowledge, this is the first study that described the prognostic significance of the aforementioned immunological parameters before and after pregnancy, and highlighted the correlation of NKT-like cells and the candidate Th1 cytokines with pregnancy loss in women with URM. Conclusions: DPNKT-like cells, IFN-γ and IL-2 patient profiles could be used as markers to predict the risk of miscarriage in patients with URM.
format	article
author	Wafaa S. Khalaf Mohammad R.A. Mahmoud Walid F. Elkhatib Hany R. Hashem Wafaa E. Soliman
author_facet	Wafaa S. Khalaf Mohammad R.A. Mahmoud Walid F. Elkhatib Hany R. Hashem Wafaa E. Soliman
author_sort	Wafaa S. Khalaf
title	Phenotypic characterization of NKT-like cells and evaluation of specifically related cytokines for the prediction of unexplained recurrent miscarriage
title_short	Phenotypic characterization of NKT-like cells and evaluation of specifically related cytokines for the prediction of unexplained recurrent miscarriage
title_full	Phenotypic characterization of NKT-like cells and evaluation of specifically related cytokines for the prediction of unexplained recurrent miscarriage
title_fullStr	Phenotypic characterization of NKT-like cells and evaluation of specifically related cytokines for the prediction of unexplained recurrent miscarriage
title_full_unstemmed	Phenotypic characterization of NKT-like cells and evaluation of specifically related cytokines for the prediction of unexplained recurrent miscarriage
title_sort	phenotypic characterization of nkt-like cells and evaluation of specifically related cytokines for the prediction of unexplained recurrent miscarriage
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/c0eb57d4a62644578221a51067282bbd
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Phenotypic characterization of NKT-like cells and evaluation of specifically related cytokines for the prediction of unexplained recurrent miscarriage

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