Adhesion of annexin 7 deficient erythrocytes to endothelial cells.

Annexin 7 deficiency has previously been shown to foster suicidal death of erythrocytes or eryptosis, which is triggered by increase of intracellular Ca(2+) concentration ([Ca(2+)](i)) and characterized by cell shrinkage and cell membrane scrambling with subsequent phosphatidylserine exposure at the...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Majed Abed, Siraskar Balasaheb, Syeda Tasneem Towhid, Christoph Daniel, Kerstin Amann, Florian Lang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c0f33896115b4c6f8c76b81bdd711dd4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c0f33896115b4c6f8c76b81bdd711dd4
record_format	dspace
spelling	oai:doaj.org-article:c0f33896115b4c6f8c76b81bdd711dd42021-11-18T07:56:50ZAdhesion of annexin 7 deficient erythrocytes to endothelial cells.1932-620310.1371/journal.pone.0056650https://doaj.org/article/c0f33896115b4c6f8c76b81bdd711dd42013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23437197/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Annexin 7 deficiency has previously been shown to foster suicidal death of erythrocytes or eryptosis, which is triggered by increase of intracellular Ca(2+) concentration ([Ca(2+)](i)) and characterized by cell shrinkage and cell membrane scrambling with subsequent phosphatidylserine exposure at the cell surface. Eryptosis following increase of [Ca(2+)](i) by Ca(2+) ionophore ionomycin, osmotic shock or energy depletion was more pronounced in erythrocytes from annexinA7-deficient mice (anxA7(-/-)) than in erythrocytes from wild type mice (anxA7(+/+)). As phosphatidylserine exposure is considered to mediate adhesion of erythrocytes to the vascular wall, the present study explored adhesion of erythrocytes from anx7(-/-) and anx7(+/+)-mice following increase of [Ca(2+)](i) by Ca(2+) ionophore ionomycin (1 µM for 30 min), hyperosmotic shock (addition of 550 mM sucrose for 2 hours) or energy depletion (removal of glucose for 12 hours). Phosphatidylserine exposing erythrocytes were identified by annexin V binding, cell volume estimated from forward scatter in FACS analysis and adhesion to human umbilical vein endothelial cells (HUVEC) utilizing a flow chamber. As a result, ionomycin, sucrose addition and glucose removal all triggered phosphatidylserine-exposure, decreased forward scatter and enhanced adhesion of erythrocytes to human umbilical vein endothelial cells (HUVEC), effects significantly more pronounced in anx7(-/-) than in anx7(+/+)-erythrocytes. Following ischemia, morphological renal injury was significantly higher in anx7(-/-) than in anx7(+/+)-mice. The present observations demonstrate that enhanced eryptosis of annexin7 deficient cells is paralleled by increased adhesion of erythrocytes to the vascular wall, an effect, which may impact on microcirculation during ischemia.Majed AbedSiraskar BalasahebSyeda Tasneem TowhidChristoph DanielKerstin AmannFlorian LangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 2, p e56650 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Majed Abed Siraskar Balasaheb Syeda Tasneem Towhid Christoph Daniel Kerstin Amann Florian Lang Adhesion of annexin 7 deficient erythrocytes to endothelial cells.
description	Annexin 7 deficiency has previously been shown to foster suicidal death of erythrocytes or eryptosis, which is triggered by increase of intracellular Ca(2+) concentration ([Ca(2+)](i)) and characterized by cell shrinkage and cell membrane scrambling with subsequent phosphatidylserine exposure at the cell surface. Eryptosis following increase of [Ca(2+)](i) by Ca(2+) ionophore ionomycin, osmotic shock or energy depletion was more pronounced in erythrocytes from annexinA7-deficient mice (anxA7(-/-)) than in erythrocytes from wild type mice (anxA7(+/+)). As phosphatidylserine exposure is considered to mediate adhesion of erythrocytes to the vascular wall, the present study explored adhesion of erythrocytes from anx7(-/-) and anx7(+/+)-mice following increase of [Ca(2+)](i) by Ca(2+) ionophore ionomycin (1 µM for 30 min), hyperosmotic shock (addition of 550 mM sucrose for 2 hours) or energy depletion (removal of glucose for 12 hours). Phosphatidylserine exposing erythrocytes were identified by annexin V binding, cell volume estimated from forward scatter in FACS analysis and adhesion to human umbilical vein endothelial cells (HUVEC) utilizing a flow chamber. As a result, ionomycin, sucrose addition and glucose removal all triggered phosphatidylserine-exposure, decreased forward scatter and enhanced adhesion of erythrocytes to human umbilical vein endothelial cells (HUVEC), effects significantly more pronounced in anx7(-/-) than in anx7(+/+)-erythrocytes. Following ischemia, morphological renal injury was significantly higher in anx7(-/-) than in anx7(+/+)-mice. The present observations demonstrate that enhanced eryptosis of annexin7 deficient cells is paralleled by increased adhesion of erythrocytes to the vascular wall, an effect, which may impact on microcirculation during ischemia.
format	article
author	Majed Abed Siraskar Balasaheb Syeda Tasneem Towhid Christoph Daniel Kerstin Amann Florian Lang
author_facet	Majed Abed Siraskar Balasaheb Syeda Tasneem Towhid Christoph Daniel Kerstin Amann Florian Lang
author_sort	Majed Abed
title	Adhesion of annexin 7 deficient erythrocytes to endothelial cells.
title_short	Adhesion of annexin 7 deficient erythrocytes to endothelial cells.
title_full	Adhesion of annexin 7 deficient erythrocytes to endothelial cells.
title_fullStr	Adhesion of annexin 7 deficient erythrocytes to endothelial cells.
title_full_unstemmed	Adhesion of annexin 7 deficient erythrocytes to endothelial cells.
title_sort	adhesion of annexin 7 deficient erythrocytes to endothelial cells.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/c0f33896115b4c6f8c76b81bdd711dd4
work_keys_str_mv	AT majedabed adhesionofannexin7deficienterythrocytestoendothelialcells AT siraskarbalasaheb adhesionofannexin7deficienterythrocytestoendothelialcells AT syedatasneemtowhid adhesionofannexin7deficienterythrocytestoendothelialcells AT christophdaniel adhesionofannexin7deficienterythrocytestoendothelialcells AT kerstinamann adhesionofannexin7deficienterythrocytestoendothelialcells AT florianlang adhesionofannexin7deficienterythrocytestoendothelialcells
_version_	1718422754984722432

Adhesion of annexin 7 deficient erythrocytes to endothelial cells.

Ejemplares similares