Propolis particles incorporated in aqueous formulations with enhanced antibacterial performance

In recent years, the use of natural bioactives in food, pharmaceutical and cosmetic industries has emerged as a global formulation development trend. Although natural bioactives exhibit promising properties, they are also associated with chemical instability or poor aqueous solubility. One such bioa...

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Autores principales:	Nelli Chourmouziadi Laleni, Paulo De Carvalho Gomes, Konstantinos Gkatzionis, Fotis Spyropoulos
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Propolis Sonication Dispersion Antibacterial activity Flow cytometry Nutrition. Foods and food supply TX341-641 Nutritional diseases. Deficiency diseases RC620-627
Acceso en línea:	https://doaj.org/article/c0fa10b50f58472da89cb5f32fc72a46
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spelling	oai:doaj.org-article:c0fa10b50f58472da89cb5f32fc72a462021-12-02T05:04:33ZPropolis particles incorporated in aqueous formulations with enhanced antibacterial performance2667-025910.1016/j.fhfh.2021.100040https://doaj.org/article/c0fa10b50f58472da89cb5f32fc72a462021-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2667025921000327https://doaj.org/toc/2667-0259In recent years, the use of natural bioactives in food, pharmaceutical and cosmetic industries has emerged as a global formulation development trend. Although natural bioactives exhibit promising properties, they are also associated with chemical instability or poor aqueous solubility. One such bioactive with beneficial functionalities but limited industrial applicability within industry is propolis. The purpose of this study was to investigate means to enable enhancement to the antibacterial activity of propolis-based aqueous formulations. Dry propolis was firstly extracted from crude material and the effect of common carrier phases used for dissolution of propolis for antibacterial assays was investigated. Consequently, the extract was formulated into propolis sub-micron aqueous dispersions via direct ultrasonication. Processing time was varied, and all formed particles were characterised immediately after production in terms of size, polydispersity and zeta potential, and then again after a month-long storage period. When tested on E. coli cells, 15% propolis dispersions caused a bactericidal effect, which was sonication time and time of exposure dependent. Particles formed at the shortest sonication period (4 min) resulted in higher cell injury while those processed the longest (10 min) caused greater cell death and with AFM imaging, cell membrane alterations were confirmed. Chemically, for whole dispersions and carrier phases alone, free radical scavenging activity and total phenol content were slightly enhanced at longer sonication times. Overall, the present work suggests that formulating propolis extract sub-micron aqueous dispersions via sonication enhances their antibacterial performance via a synergistic effect involving both their carrier and dispersed phases.Nelli Chourmouziadi LaleniPaulo De Carvalho GomesKonstantinos GkatzionisFotis SpyropoulosElsevierarticlePropolisSonicationDispersionAntibacterial activityFlow cytometryNutrition. Foods and food supplyTX341-641Nutritional diseases. Deficiency diseasesRC620-627ENFood Hydrocolloids for Health, Vol 1, Iss , Pp 100040- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Propolis Sonication Dispersion Antibacterial activity Flow cytometry Nutrition. Foods and food supply TX341-641 Nutritional diseases. Deficiency diseases RC620-627
spellingShingle	Propolis Sonication Dispersion Antibacterial activity Flow cytometry Nutrition. Foods and food supply TX341-641 Nutritional diseases. Deficiency diseases RC620-627 Nelli Chourmouziadi Laleni Paulo De Carvalho Gomes Konstantinos Gkatzionis Fotis Spyropoulos Propolis particles incorporated in aqueous formulations with enhanced antibacterial performance
description	In recent years, the use of natural bioactives in food, pharmaceutical and cosmetic industries has emerged as a global formulation development trend. Although natural bioactives exhibit promising properties, they are also associated with chemical instability or poor aqueous solubility. One such bioactive with beneficial functionalities but limited industrial applicability within industry is propolis. The purpose of this study was to investigate means to enable enhancement to the antibacterial activity of propolis-based aqueous formulations. Dry propolis was firstly extracted from crude material and the effect of common carrier phases used for dissolution of propolis for antibacterial assays was investigated. Consequently, the extract was formulated into propolis sub-micron aqueous dispersions via direct ultrasonication. Processing time was varied, and all formed particles were characterised immediately after production in terms of size, polydispersity and zeta potential, and then again after a month-long storage period. When tested on E. coli cells, 15% propolis dispersions caused a bactericidal effect, which was sonication time and time of exposure dependent. Particles formed at the shortest sonication period (4 min) resulted in higher cell injury while those processed the longest (10 min) caused greater cell death and with AFM imaging, cell membrane alterations were confirmed. Chemically, for whole dispersions and carrier phases alone, free radical scavenging activity and total phenol content were slightly enhanced at longer sonication times. Overall, the present work suggests that formulating propolis extract sub-micron aqueous dispersions via sonication enhances their antibacterial performance via a synergistic effect involving both their carrier and dispersed phases.
format	article
author	Nelli Chourmouziadi Laleni Paulo De Carvalho Gomes Konstantinos Gkatzionis Fotis Spyropoulos
author_facet	Nelli Chourmouziadi Laleni Paulo De Carvalho Gomes Konstantinos Gkatzionis Fotis Spyropoulos
author_sort	Nelli Chourmouziadi Laleni
title	Propolis particles incorporated in aqueous formulations with enhanced antibacterial performance
title_short	Propolis particles incorporated in aqueous formulations with enhanced antibacterial performance
title_full	Propolis particles incorporated in aqueous formulations with enhanced antibacterial performance
title_fullStr	Propolis particles incorporated in aqueous formulations with enhanced antibacterial performance
title_full_unstemmed	Propolis particles incorporated in aqueous formulations with enhanced antibacterial performance
title_sort	propolis particles incorporated in aqueous formulations with enhanced antibacterial performance
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/c0fa10b50f58472da89cb5f32fc72a46
work_keys_str_mv	AT nellichourmouziadilaleni propolisparticlesincorporatedinaqueousformulationswithenhancedantibacterialperformance AT paulodecarvalhogomes propolisparticlesincorporatedinaqueousformulationswithenhancedantibacterialperformance AT konstantinosgkatzionis propolisparticlesincorporatedinaqueousformulationswithenhancedantibacterialperformance AT fotisspyropoulos propolisparticlesincorporatedinaqueousformulationswithenhancedantibacterialperformance
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Propolis particles incorporated in aqueous formulations with enhanced antibacterial performance

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