A MAP6-related protein is present in protozoa and is involved in flagellum motility.

In vertebrates the microtubule-associated proteins MAP6 and MAP6d1 stabilize cold-resistant microtubules. Cilia and flagella have cold-stable microtubules but MAP6 proteins have not been identified in these organelles. Here, we describe TbSAXO as the first MAP6-related protein to be identified in a...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Denis Dacheux, Nicolas Landrein, Magali Thonnus, Guillaume Gilbert, Annelise Sahin, Harald Wodrich, Derrick R Robinson, Mélanie Bonhivers
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c1031b405a364705bbbb5c9048c489d1
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c1031b405a364705bbbb5c9048c489d1
record_format	dspace
spelling	oai:doaj.org-article:c1031b405a364705bbbb5c9048c489d12021-11-18T07:28:11ZA MAP6-related protein is present in protozoa and is involved in flagellum motility.1932-620310.1371/journal.pone.0031344https://doaj.org/article/c1031b405a364705bbbb5c9048c489d12012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22355359/?tool=EBIhttps://doaj.org/toc/1932-6203In vertebrates the microtubule-associated proteins MAP6 and MAP6d1 stabilize cold-resistant microtubules. Cilia and flagella have cold-stable microtubules but MAP6 proteins have not been identified in these organelles. Here, we describe TbSAXO as the first MAP6-related protein to be identified in a protozoan, Trypanosoma brucei. Using a heterologous expression system, we show that TbSAXO is a microtubule stabilizing protein. Furthermore we identify the domains of the protein responsible for microtubule binding and stabilizing and show that they share homologies with the microtubule-stabilizing Mn domains of the MAP6 proteins. We demonstrate, in the flagellated parasite, that TbSAXO is an axonemal protein that plays a role in flagellum motility. Lastly we provide evidence that TbSAXO belongs to a group of MAP6-related proteins (SAXO proteins) present only in ciliated or flagellated organisms ranging from protozoa to mammals. We discuss the potential roles of the SAXO proteins in cilia and flagella function.Denis DacheuxNicolas LandreinMagali ThonnusGuillaume GilbertAnnelise SahinHarald WodrichDerrick R RobinsonMélanie BonhiversPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 2, p e31344 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Denis Dacheux Nicolas Landrein Magali Thonnus Guillaume Gilbert Annelise Sahin Harald Wodrich Derrick R Robinson Mélanie Bonhivers A MAP6-related protein is present in protozoa and is involved in flagellum motility.
description	In vertebrates the microtubule-associated proteins MAP6 and MAP6d1 stabilize cold-resistant microtubules. Cilia and flagella have cold-stable microtubules but MAP6 proteins have not been identified in these organelles. Here, we describe TbSAXO as the first MAP6-related protein to be identified in a protozoan, Trypanosoma brucei. Using a heterologous expression system, we show that TbSAXO is a microtubule stabilizing protein. Furthermore we identify the domains of the protein responsible for microtubule binding and stabilizing and show that they share homologies with the microtubule-stabilizing Mn domains of the MAP6 proteins. We demonstrate, in the flagellated parasite, that TbSAXO is an axonemal protein that plays a role in flagellum motility. Lastly we provide evidence that TbSAXO belongs to a group of MAP6-related proteins (SAXO proteins) present only in ciliated or flagellated organisms ranging from protozoa to mammals. We discuss the potential roles of the SAXO proteins in cilia and flagella function.
format	article
author	Denis Dacheux Nicolas Landrein Magali Thonnus Guillaume Gilbert Annelise Sahin Harald Wodrich Derrick R Robinson Mélanie Bonhivers
author_facet	Denis Dacheux Nicolas Landrein Magali Thonnus Guillaume Gilbert Annelise Sahin Harald Wodrich Derrick R Robinson Mélanie Bonhivers
author_sort	Denis Dacheux
title	A MAP6-related protein is present in protozoa and is involved in flagellum motility.
title_short	A MAP6-related protein is present in protozoa and is involved in flagellum motility.
title_full	A MAP6-related protein is present in protozoa and is involved in flagellum motility.
title_fullStr	A MAP6-related protein is present in protozoa and is involved in flagellum motility.
title_full_unstemmed	A MAP6-related protein is present in protozoa and is involved in flagellum motility.
title_sort	map6-related protein is present in protozoa and is involved in flagellum motility.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/c1031b405a364705bbbb5c9048c489d1
work_keys_str_mv	AT denisdacheux amap6relatedproteinispresentinprotozoaandisinvolvedinflagellummotility AT nicolaslandrein amap6relatedproteinispresentinprotozoaandisinvolvedinflagellummotility AT magalithonnus amap6relatedproteinispresentinprotozoaandisinvolvedinflagellummotility AT guillaumegilbert amap6relatedproteinispresentinprotozoaandisinvolvedinflagellummotility AT annelisesahin amap6relatedproteinispresentinprotozoaandisinvolvedinflagellummotility AT haraldwodrich amap6relatedproteinispresentinprotozoaandisinvolvedinflagellummotility AT derrickrrobinson amap6relatedproteinispresentinprotozoaandisinvolvedinflagellummotility AT melaniebonhivers amap6relatedproteinispresentinprotozoaandisinvolvedinflagellummotility AT denisdacheux map6relatedproteinispresentinprotozoaandisinvolvedinflagellummotility AT nicolaslandrein map6relatedproteinispresentinprotozoaandisinvolvedinflagellummotility AT magalithonnus map6relatedproteinispresentinprotozoaandisinvolvedinflagellummotility AT guillaumegilbert map6relatedproteinispresentinprotozoaandisinvolvedinflagellummotility AT annelisesahin map6relatedproteinispresentinprotozoaandisinvolvedinflagellummotility AT haraldwodrich map6relatedproteinispresentinprotozoaandisinvolvedinflagellummotility AT derrickrrobinson map6relatedproteinispresentinprotozoaandisinvolvedinflagellummotility AT melaniebonhivers map6relatedproteinispresentinprotozoaandisinvolvedinflagellummotility
_version_	1718423436313755648

A MAP6-related protein is present in protozoa and is involved in flagellum motility.

Ejemplares similares