A Sesquiterpene Aldehyde Isolated From Ethyl Acetate Extract of Lansium Domesticum Fruit Peel

Lansium domesticum (fam. Meliaceae) contains various compounds with various biological activities. Based on the previous research, extracts from several parts of the plant have biological activity. This study aimed to isolate a compound from the fruitpeel of L. domesticum and evaluate cytotoxic acti...

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Autores principales:	Khusnul Fadhilah, Subagus Wahyuono, Puji Astuti
Formato:	article
Lenguaje:	EN
Publicado:	Universitas Gadjah Mada 2021
Materias:	lansium domesticum, sesquiterpene, cytotoxic, cancer Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/c105875d37714edb8b67331b533d4d6a
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spelling	oai:doaj.org-article:c105875d37714edb8b67331b533d4d6a2021-11-15T06:08:46ZA Sesquiterpene Aldehyde Isolated From Ethyl Acetate Extract of Lansium Domesticum Fruit Peel2338-94272338-948610.22146/ijp.1884https://doaj.org/article/c105875d37714edb8b67331b533d4d6a2021-09-01T00:00:00Zhttps://jurnal.ugm.ac.id/v3/IJP/article/view/1884https://doaj.org/toc/2338-9427https://doaj.org/toc/2338-9486Lansium domesticum (fam. Meliaceae) contains various compounds with various biological activities. Based on the previous research, extracts from several parts of the plant have biological activity. This study aimed to isolate a compound from the fruitpeel of L. domesticum and evaluate cytotoxic activity against T47D, WiDr and HepG2 cell lines. Powdered peels were macerated with ethyl acetate and the filtrate was evaporated to give EtOAc extract. Dried extract was triturated with n-hexane to give n-hexane soluble fraction (A) and insoluble fraction (B). The fraction B was separated using vacuum column chromatography (VLC) with mobile phase n-hexane: ethyl acetate and given 5 fractions. Fractions B3-B5 were combined and separated using VLC with n-hexane and ethyl acetate as mobile phase. This VLC separation gave 18 subractions, subfractions 6-9 with the similar TLC profile were combined. This subfraction was separated further using preparative thin layer chromatography to give compound 1. The Isolated compound (1) appeared as liquid. The chemical structure of 1 was identified acoording to spectroscopic data and comparison with literature. Cytotoxic bioassay was performed on T-47D, WiDr and Hep G2 cell lines in a series of concentrations at 50, 40, 30, 20, 10 and 5µg/mL, with Doxorubicine used as positive control. According to spectroscopic data, compound 1 was identified as 2-ethyl,3-(1’-hydroxy-2’-menthene) propenal, and demonstrate the strongest cytotoxicity against T-47D cell lines (IC50=39.18+1.54 µg/mL).Khusnul FadhilahSubagus WahyuonoPuji AstutiUniversitas Gadjah Madaarticlelansium domesticum, sesquiterpene, cytotoxic, cancerPharmacy and materia medicaRS1-441ENIndonesian Journal of Pharmacy, Pp 394-398 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	lansium domesticum, sesquiterpene, cytotoxic, cancer Pharmacy and materia medica RS1-441
spellingShingle	lansium domesticum, sesquiterpene, cytotoxic, cancer Pharmacy and materia medica RS1-441 Khusnul Fadhilah Subagus Wahyuono Puji Astuti A Sesquiterpene Aldehyde Isolated From Ethyl Acetate Extract of Lansium Domesticum Fruit Peel
description	Lansium domesticum (fam. Meliaceae) contains various compounds with various biological activities. Based on the previous research, extracts from several parts of the plant have biological activity. This study aimed to isolate a compound from the fruitpeel of L. domesticum and evaluate cytotoxic activity against T47D, WiDr and HepG2 cell lines. Powdered peels were macerated with ethyl acetate and the filtrate was evaporated to give EtOAc extract. Dried extract was triturated with n-hexane to give n-hexane soluble fraction (A) and insoluble fraction (B). The fraction B was separated using vacuum column chromatography (VLC) with mobile phase n-hexane: ethyl acetate and given 5 fractions. Fractions B3-B5 were combined and separated using VLC with n-hexane and ethyl acetate as mobile phase. This VLC separation gave 18 subractions, subfractions 6-9 with the similar TLC profile were combined. This subfraction was separated further using preparative thin layer chromatography to give compound 1. The Isolated compound (1) appeared as liquid. The chemical structure of 1 was identified acoording to spectroscopic data and comparison with literature. Cytotoxic bioassay was performed on T-47D, WiDr and Hep G2 cell lines in a series of concentrations at 50, 40, 30, 20, 10 and 5µg/mL, with Doxorubicine used as positive control. According to spectroscopic data, compound 1 was identified as 2-ethyl,3-(1’-hydroxy-2’-menthene) propenal, and demonstrate the strongest cytotoxicity against T-47D cell lines (IC50=39.18+1.54 µg/mL).
format	article
author	Khusnul Fadhilah Subagus Wahyuono Puji Astuti
author_facet	Khusnul Fadhilah Subagus Wahyuono Puji Astuti
author_sort	Khusnul Fadhilah
title	A Sesquiterpene Aldehyde Isolated From Ethyl Acetate Extract of Lansium Domesticum Fruit Peel
title_short	A Sesquiterpene Aldehyde Isolated From Ethyl Acetate Extract of Lansium Domesticum Fruit Peel
title_full	A Sesquiterpene Aldehyde Isolated From Ethyl Acetate Extract of Lansium Domesticum Fruit Peel
title_fullStr	A Sesquiterpene Aldehyde Isolated From Ethyl Acetate Extract of Lansium Domesticum Fruit Peel
title_full_unstemmed	A Sesquiterpene Aldehyde Isolated From Ethyl Acetate Extract of Lansium Domesticum Fruit Peel
title_sort	sesquiterpene aldehyde isolated from ethyl acetate extract of lansium domesticum fruit peel
publisher	Universitas Gadjah Mada
publishDate	2021
url	https://doaj.org/article/c105875d37714edb8b67331b533d4d6a
work_keys_str_mv	AT khusnulfadhilah asesquiterpenealdehydeisolatedfromethylacetateextractoflansiumdomesticumfruitpeel AT subaguswahyuono asesquiterpenealdehydeisolatedfromethylacetateextractoflansiumdomesticumfruitpeel AT pujiastuti asesquiterpenealdehydeisolatedfromethylacetateextractoflansiumdomesticumfruitpeel AT khusnulfadhilah sesquiterpenealdehydeisolatedfromethylacetateextractoflansiumdomesticumfruitpeel AT subaguswahyuono sesquiterpenealdehydeisolatedfromethylacetateextractoflansiumdomesticumfruitpeel AT pujiastuti sesquiterpenealdehydeisolatedfromethylacetateextractoflansiumdomesticumfruitpeel
_version_	1718428580025729024

A Sesquiterpene Aldehyde Isolated From Ethyl Acetate Extract of Lansium Domesticum Fruit Peel

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