3-Bromofluoranthene-induced cardiotoxicity of zebrafish and apoptosis in the vascular endothelial cells via intrinsic and extrinsic caspase-dependent pathways

Fluoranthene, a high-molecular-weight polycyclic aromatic hydrocarbon (PAH), is widely present in air pollutants, including fine inhalable particulate matter. 3-Bromofluoranthene (3-BrFlu), which is a brominated fluoranthene and halogenated PAH, is generated from waste combustion, metallurgical proc...

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Autores principales:	Chun-Hung Su, Shih-Pin Chen, Li-You Chen, Jiann-Jou Yang, Yi-Chia Lee, Shiuan-Shinn Lee, Hsin-Hung Chen, Yan-Yan Ng, Yu-Hsiang Kuan
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	3-Bromofluoranthene Zebrafish Vascular endothelial cells Caspase-dependent apoptosis Environmental pollution TD172-193.5 Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/c11625813d6c4bababff5cabc9fca5b5
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spelling	oai:doaj.org-article:c11625813d6c4bababff5cabc9fca5b52021-11-12T04:24:06Z3-Bromofluoranthene-induced cardiotoxicity of zebrafish and apoptosis in the vascular endothelial cells via intrinsic and extrinsic caspase-dependent pathways0147-651310.1016/j.ecoenv.2021.112962https://doaj.org/article/c11625813d6c4bababff5cabc9fca5b52021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0147651321010745https://doaj.org/toc/0147-6513Fluoranthene, a high-molecular-weight polycyclic aromatic hydrocarbon (PAH), is widely present in air pollutants, including fine inhalable particulate matter. 3-Bromofluoranthene (3-BrFlu), which is a brominated fluoranthene and halogenated PAH, is generated from waste combustion, metallurgical processes, cement production, e-waste dismantling, and photoreaction. Vascular endothelial cells have key functions in the homeostasis and the development of the cardiovascular system. The zebrafish model has been widely employed to study cardiotoxicity and embryotoxicity. However, no evidence has indicated that 3-BrFlu induces cytotoxicity in vascular endothelial cells, or cardiotoxicity and embryotoxicity in zebrafish. In this study, 3-BrFlu induced concentration-dependent changes in embryo- and cardiotoxicity. Cytotoxicity was also induced by 3-BrFlu in a concentration-dependent manner through apoptosis and necrosis in vascular endothelial cells, SVEC4-10 cells. The activities of caspase-3, -8, and -9 were induced by 3-BrFlu via an intrinsic pathway constituting Bcl-2 downregulation, Bad upregulation, and mitochondrial dysfunction; the extrinsic pathway included the expression of death receptors, including tumour necrosis factor α and Fas receptors. These results indicated that 3-BrFlu caused cardio- and embryotoxicity in zebrafish through vascular endothelial cells cytotoxicity resulting from caspase-dependent apoptosis through intrinsic and extrinsic pathways.Chun-Hung SuShih-Pin ChenLi-You ChenJiann-Jou YangYi-Chia LeeShiuan-Shinn LeeHsin-Hung ChenYan-Yan NgYu-Hsiang KuanElsevierarticle3-BromofluorantheneZebrafishVascular endothelial cellsCaspase-dependent apoptosisEnvironmental pollutionTD172-193.5Environmental sciencesGE1-350ENEcotoxicology and Environmental Safety, Vol 228, Iss , Pp 112962- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	3-Bromofluoranthene Zebrafish Vascular endothelial cells Caspase-dependent apoptosis Environmental pollution TD172-193.5 Environmental sciences GE1-350
spellingShingle	3-Bromofluoranthene Zebrafish Vascular endothelial cells Caspase-dependent apoptosis Environmental pollution TD172-193.5 Environmental sciences GE1-350 Chun-Hung Su Shih-Pin Chen Li-You Chen Jiann-Jou Yang Yi-Chia Lee Shiuan-Shinn Lee Hsin-Hung Chen Yan-Yan Ng Yu-Hsiang Kuan 3-Bromofluoranthene-induced cardiotoxicity of zebrafish and apoptosis in the vascular endothelial cells via intrinsic and extrinsic caspase-dependent pathways
description	Fluoranthene, a high-molecular-weight polycyclic aromatic hydrocarbon (PAH), is widely present in air pollutants, including fine inhalable particulate matter. 3-Bromofluoranthene (3-BrFlu), which is a brominated fluoranthene and halogenated PAH, is generated from waste combustion, metallurgical processes, cement production, e-waste dismantling, and photoreaction. Vascular endothelial cells have key functions in the homeostasis and the development of the cardiovascular system. The zebrafish model has been widely employed to study cardiotoxicity and embryotoxicity. However, no evidence has indicated that 3-BrFlu induces cytotoxicity in vascular endothelial cells, or cardiotoxicity and embryotoxicity in zebrafish. In this study, 3-BrFlu induced concentration-dependent changes in embryo- and cardiotoxicity. Cytotoxicity was also induced by 3-BrFlu in a concentration-dependent manner through apoptosis and necrosis in vascular endothelial cells, SVEC4-10 cells. The activities of caspase-3, -8, and -9 were induced by 3-BrFlu via an intrinsic pathway constituting Bcl-2 downregulation, Bad upregulation, and mitochondrial dysfunction; the extrinsic pathway included the expression of death receptors, including tumour necrosis factor α and Fas receptors. These results indicated that 3-BrFlu caused cardio- and embryotoxicity in zebrafish through vascular endothelial cells cytotoxicity resulting from caspase-dependent apoptosis through intrinsic and extrinsic pathways.
format	article
author	Chun-Hung Su Shih-Pin Chen Li-You Chen Jiann-Jou Yang Yi-Chia Lee Shiuan-Shinn Lee Hsin-Hung Chen Yan-Yan Ng Yu-Hsiang Kuan
author_facet	Chun-Hung Su Shih-Pin Chen Li-You Chen Jiann-Jou Yang Yi-Chia Lee Shiuan-Shinn Lee Hsin-Hung Chen Yan-Yan Ng Yu-Hsiang Kuan
author_sort	Chun-Hung Su
title	3-Bromofluoranthene-induced cardiotoxicity of zebrafish and apoptosis in the vascular endothelial cells via intrinsic and extrinsic caspase-dependent pathways
title_short	3-Bromofluoranthene-induced cardiotoxicity of zebrafish and apoptosis in the vascular endothelial cells via intrinsic and extrinsic caspase-dependent pathways
title_full	3-Bromofluoranthene-induced cardiotoxicity of zebrafish and apoptosis in the vascular endothelial cells via intrinsic and extrinsic caspase-dependent pathways
title_fullStr	3-Bromofluoranthene-induced cardiotoxicity of zebrafish and apoptosis in the vascular endothelial cells via intrinsic and extrinsic caspase-dependent pathways
title_full_unstemmed	3-Bromofluoranthene-induced cardiotoxicity of zebrafish and apoptosis in the vascular endothelial cells via intrinsic and extrinsic caspase-dependent pathways
title_sort	3-bromofluoranthene-induced cardiotoxicity of zebrafish and apoptosis in the vascular endothelial cells via intrinsic and extrinsic caspase-dependent pathways
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/c11625813d6c4bababff5cabc9fca5b5
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3-Bromofluoranthene-induced cardiotoxicity of zebrafish and apoptosis in the vascular endothelial cells via intrinsic and extrinsic caspase-dependent pathways

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