CRISPR/Cas9-mediated genome editing in rice cv. IPB3S results in a semi-dwarf phenotypic mutant

Abstract. Nurhayati, Ardie SW, Santoso TJ, Sudarsono. 2021. CRISPR/Cas9-mediated genome editing in rice cv. IPB3S results in a semi-dwarf phenotypic mutant. Biodiversitas 22: 3792-3800. IPB3S is Indonesian lowland rice and high-yielding cultivar. However, plant height posture makes it prone to lodgi...

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Autores principales:	NURHAYATI NURHAYATI, Sintho Wahyuning Ardie, Tri Joko Santoso, Sudarsono Sudarsono
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2021
Materias:	agrobacterium tumefaciens semi dwarf rice ga20ox2 gene genome editing crispr/cas9 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/c119d6272c8848318cc09b20795c81ce
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spelling	oai:doaj.org-article:c119d6272c8848318cc09b20795c81ce2021-11-22T12:18:55ZCRISPR/Cas9-mediated genome editing in rice cv. IPB3S results in a semi-dwarf phenotypic mutant1412-033X2085-472210.13057/biodiv/d220924https://doaj.org/article/c119d6272c8848318cc09b20795c81ce2021-08-01T00:00:00Zhttps://smujo.id/biodiv/article/view/9132https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Nurhayati, Ardie SW, Santoso TJ, Sudarsono. 2021. CRISPR/Cas9-mediated genome editing in rice cv. IPB3S results in a semi-dwarf phenotypic mutant. Biodiversitas 22: 3792-3800. IPB3S is Indonesian lowland rice and high-yielding cultivar. However, plant height posture makes it prone to lodging which could reduce the yield. This study aimed to edit the GA20Ox2 gene by introducing CRISPR/Cas9 GA20Ox2 construct into IPB3S and developing the semidwarf rice mutants. Immature embryo explants of IPB3S were used for the transformation process mediated by EHA105 strain of Agrobacterium tumefaciens carrying pC1300-Cas9/GA20Ox2 and by changing the regeneration medium composition. PCR analysis showed that rice cv. IPB3S genetic transformation gained putative mutant T0 lines carrying the hpt gene (growth efficiency was 47.9%, while transformation efficiency was 19.3%). Using the developed regeneration medium, we have obtained 24 putative rice cv. IPB3S T0 mutant lines carrying hpt. The best medium for regenerating IPB3S was the A medium (regeneration efficiency 73.3%). IPB 8 and IPB 14 were potential to evaluate in the next generation. The shortest plant height for the T1 generation was observed in the IPB 8-3 mutant.NURHAYATI NURHAYATISintho Wahyuning ArdieTri Joko SantosoSudarsono SudarsonoMBI & UNS Soloarticleagrobacterium tumefacienssemi dwarf ricega20ox2 genegenome editingcrispr/cas9Biology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	agrobacterium tumefaciens semi dwarf rice ga20ox2 gene genome editing crispr/cas9 Biology (General) QH301-705.5
spellingShingle	agrobacterium tumefaciens semi dwarf rice ga20ox2 gene genome editing crispr/cas9 Biology (General) QH301-705.5 NURHAYATI NURHAYATI Sintho Wahyuning Ardie Tri Joko Santoso Sudarsono Sudarsono CRISPR/Cas9-mediated genome editing in rice cv. IPB3S results in a semi-dwarf phenotypic mutant
description	Abstract. Nurhayati, Ardie SW, Santoso TJ, Sudarsono. 2021. CRISPR/Cas9-mediated genome editing in rice cv. IPB3S results in a semi-dwarf phenotypic mutant. Biodiversitas 22: 3792-3800. IPB3S is Indonesian lowland rice and high-yielding cultivar. However, plant height posture makes it prone to lodging which could reduce the yield. This study aimed to edit the GA20Ox2 gene by introducing CRISPR/Cas9 GA20Ox2 construct into IPB3S and developing the semidwarf rice mutants. Immature embryo explants of IPB3S were used for the transformation process mediated by EHA105 strain of Agrobacterium tumefaciens carrying pC1300-Cas9/GA20Ox2 and by changing the regeneration medium composition. PCR analysis showed that rice cv. IPB3S genetic transformation gained putative mutant T0 lines carrying the hpt gene (growth efficiency was 47.9%, while transformation efficiency was 19.3%). Using the developed regeneration medium, we have obtained 24 putative rice cv. IPB3S T0 mutant lines carrying hpt. The best medium for regenerating IPB3S was the A medium (regeneration efficiency 73.3%). IPB 8 and IPB 14 were potential to evaluate in the next generation. The shortest plant height for the T1 generation was observed in the IPB 8-3 mutant.
format	article
author	NURHAYATI NURHAYATI Sintho Wahyuning Ardie Tri Joko Santoso Sudarsono Sudarsono
author_facet	NURHAYATI NURHAYATI Sintho Wahyuning Ardie Tri Joko Santoso Sudarsono Sudarsono
author_sort	NURHAYATI NURHAYATI
title	CRISPR/Cas9-mediated genome editing in rice cv. IPB3S results in a semi-dwarf phenotypic mutant
title_short	CRISPR/Cas9-mediated genome editing in rice cv. IPB3S results in a semi-dwarf phenotypic mutant
title_full	CRISPR/Cas9-mediated genome editing in rice cv. IPB3S results in a semi-dwarf phenotypic mutant
title_fullStr	CRISPR/Cas9-mediated genome editing in rice cv. IPB3S results in a semi-dwarf phenotypic mutant
title_full_unstemmed	CRISPR/Cas9-mediated genome editing in rice cv. IPB3S results in a semi-dwarf phenotypic mutant
title_sort	crispr/cas9-mediated genome editing in rice cv. ipb3s results in a semi-dwarf phenotypic mutant
publisher	MBI & UNS Solo
publishDate	2021
url	https://doaj.org/article/c119d6272c8848318cc09b20795c81ce
work_keys_str_mv	AT nurhayatinurhayati crisprcas9mediatedgenomeeditinginricecvipb3sresultsinasemidwarfphenotypicmutant AT sinthowahyuningardie crisprcas9mediatedgenomeeditinginricecvipb3sresultsinasemidwarfphenotypicmutant AT trijokosantoso crisprcas9mediatedgenomeeditinginricecvipb3sresultsinasemidwarfphenotypicmutant AT sudarsonosudarsono crisprcas9mediatedgenomeeditinginricecvipb3sresultsinasemidwarfphenotypicmutant
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CRISPR/Cas9-mediated genome editing in rice cv. IPB3S results in a semi-dwarf phenotypic mutant

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