A Luminex assay detects amyloid β oligomers in Alzheimer's disease cerebrospinal fluid.

Amyloid beta (aβ) protein assembles into larger protein aggregates during the pathogenesis of Alzheimer's disease (AD) and there is increasing evidence that soluble aβ oligomers are a critical pathologic species. Diagnostic evaluations rely on the measurement of increased tau and decreased aβ42...

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Autores principales:	Adrianna Z Herskovits, Joseph J Locascio, Elaine R Peskind, Ge Li, Bradley T Hyman
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c11db52ec3a644b586af0be0eb70ffb0
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spelling	oai:doaj.org-article:c11db52ec3a644b586af0be0eb70ffb02021-11-18T07:39:02ZA Luminex assay detects amyloid β oligomers in Alzheimer's disease cerebrospinal fluid.1932-620310.1371/journal.pone.0067898https://doaj.org/article/c11db52ec3a644b586af0be0eb70ffb02013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23844122/?tool=EBIhttps://doaj.org/toc/1932-6203Amyloid beta (aβ) protein assembles into larger protein aggregates during the pathogenesis of Alzheimer's disease (AD) and there is increasing evidence that soluble aβ oligomers are a critical pathologic species. Diagnostic evaluations rely on the measurement of increased tau and decreased aβ42 in the cerebrospinal fluid (CSF) from AD patients and evidence for oligomeric aβ in patient CSF is conflicting. In this study, we have adapted a monoclonal single antibody sandwich ELISA assay to a Luminex platform and found that this assay can detect oligomerized aβ42 and sAPPα fragments. We evaluated oligomeric aβ reactivity in 20 patients with AD relative to 19 age matched controls and compared these values with a commercially available Alzbio3 kit that detects tau, phosphorylated tau and aβ42 on the same diagnostic platform. We found that CSF samples of patients with AD had elevated aβ oligomers compared to control subjects (p < 0.05) and the ratio of aβ oligomers to aβ42 was also significantly elevated (p < 0.0001). Further research to develop high sensitivity analytical platforms and rigorous methods of developing stable assay standards will be needed before the analysis of oligomeric aβ becomes a routine diagnostic assay for the evaluation of late onset AD patients.Adrianna Z HerskovitsJoseph J LocascioElaine R PeskindGe LiBradley T HymanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e67898 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Adrianna Z Herskovits Joseph J Locascio Elaine R Peskind Ge Li Bradley T Hyman A Luminex assay detects amyloid β oligomers in Alzheimer's disease cerebrospinal fluid.
description	Amyloid beta (aβ) protein assembles into larger protein aggregates during the pathogenesis of Alzheimer's disease (AD) and there is increasing evidence that soluble aβ oligomers are a critical pathologic species. Diagnostic evaluations rely on the measurement of increased tau and decreased aβ42 in the cerebrospinal fluid (CSF) from AD patients and evidence for oligomeric aβ in patient CSF is conflicting. In this study, we have adapted a monoclonal single antibody sandwich ELISA assay to a Luminex platform and found that this assay can detect oligomerized aβ42 and sAPPα fragments. We evaluated oligomeric aβ reactivity in 20 patients with AD relative to 19 age matched controls and compared these values with a commercially available Alzbio3 kit that detects tau, phosphorylated tau and aβ42 on the same diagnostic platform. We found that CSF samples of patients with AD had elevated aβ oligomers compared to control subjects (p < 0.05) and the ratio of aβ oligomers to aβ42 was also significantly elevated (p < 0.0001). Further research to develop high sensitivity analytical platforms and rigorous methods of developing stable assay standards will be needed before the analysis of oligomeric aβ becomes a routine diagnostic assay for the evaluation of late onset AD patients.
format	article
author	Adrianna Z Herskovits Joseph J Locascio Elaine R Peskind Ge Li Bradley T Hyman
author_facet	Adrianna Z Herskovits Joseph J Locascio Elaine R Peskind Ge Li Bradley T Hyman
author_sort	Adrianna Z Herskovits
title	A Luminex assay detects amyloid β oligomers in Alzheimer's disease cerebrospinal fluid.
title_short	A Luminex assay detects amyloid β oligomers in Alzheimer's disease cerebrospinal fluid.
title_full	A Luminex assay detects amyloid β oligomers in Alzheimer's disease cerebrospinal fluid.
title_fullStr	A Luminex assay detects amyloid β oligomers in Alzheimer's disease cerebrospinal fluid.
title_full_unstemmed	A Luminex assay detects amyloid β oligomers in Alzheimer's disease cerebrospinal fluid.
title_sort	luminex assay detects amyloid β oligomers in alzheimer's disease cerebrospinal fluid.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/c11db52ec3a644b586af0be0eb70ffb0
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A Luminex assay detects amyloid β oligomers in Alzheimer's disease cerebrospinal fluid.

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