Effect Of Microgravity On Aromatase Expression In Sertoli Cells

Abstract Cytochrome P450-aromatase catalyzes estrogen biosynthesis from C19 steroids. In the testis, Sertoli cells express P450-aromatase and represent the primary source of estrogen during prepuberal age. This study focused on the effect of simulated microgravity (SM) on aromatase expression in pri...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Elisa Cirelli, Emanuela De Domenico, Flavia Botti, Renato Massoud, Raffaele Geremia, Paola Grimaldi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c1201aea0a8247aaafdddcd61764f5e6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c1201aea0a8247aaafdddcd61764f5e6
record_format	dspace
spelling	oai:doaj.org-article:c1201aea0a8247aaafdddcd61764f5e62021-12-02T16:06:39ZEffect Of Microgravity On Aromatase Expression In Sertoli Cells10.1038/s41598-017-02018-22045-2322https://doaj.org/article/c1201aea0a8247aaafdddcd61764f5e62017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02018-2https://doaj.org/toc/2045-2322Abstract Cytochrome P450-aromatase catalyzes estrogen biosynthesis from C19 steroids. In the testis, Sertoli cells express P450-aromatase and represent the primary source of estrogen during prepuberal age. This study focused on the effect of simulated microgravity (SM) on aromatase expression in primary mouse Sertoli cells. When cultured in Rotary Cell Culture System (RCCS), Sertoli cells, formed multicellular three dimensional spheroids (3D). Biological properties were first analyzed in terms of viability, cell cycle, expression of cytoskeletal components and growth factors in comparison to Sertoli cells cultured in spheroids at unit gravity (G). SM did not affect cell viability and proliferation, nor expression of the main cytoskeleton proteins and of growth factors like Kit Ligand (KL) and glial derived neurotrophic factor (GDNF). On the other hand, SM caused a strong increase in P450 aromatase mRNA and protein expression. Interestingly, P450-aromatase was no more inducible by 8-Br-cAMP. The presence of a functional aromatase was confirmed by enrichment of 17β-estradiol released in the medium by androgen precursors. We concluded that SM causes a significant upregulation of aromatase gene expression in Sertoli cells, leading to a consequent increase in 17β-estradiol secretion. High level of 17β-estradiol in the testis could have potentially adverse effects on male fertility and testicular cancer.Elisa CirelliEmanuela De DomenicoFlavia BottiRenato MassoudRaffaele GeremiaPaola GrimaldiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Elisa Cirelli Emanuela De Domenico Flavia Botti Renato Massoud Raffaele Geremia Paola Grimaldi Effect Of Microgravity On Aromatase Expression In Sertoli Cells
description	Abstract Cytochrome P450-aromatase catalyzes estrogen biosynthesis from C19 steroids. In the testis, Sertoli cells express P450-aromatase and represent the primary source of estrogen during prepuberal age. This study focused on the effect of simulated microgravity (SM) on aromatase expression in primary mouse Sertoli cells. When cultured in Rotary Cell Culture System (RCCS), Sertoli cells, formed multicellular three dimensional spheroids (3D). Biological properties were first analyzed in terms of viability, cell cycle, expression of cytoskeletal components and growth factors in comparison to Sertoli cells cultured in spheroids at unit gravity (G). SM did not affect cell viability and proliferation, nor expression of the main cytoskeleton proteins and of growth factors like Kit Ligand (KL) and glial derived neurotrophic factor (GDNF). On the other hand, SM caused a strong increase in P450 aromatase mRNA and protein expression. Interestingly, P450-aromatase was no more inducible by 8-Br-cAMP. The presence of a functional aromatase was confirmed by enrichment of 17β-estradiol released in the medium by androgen precursors. We concluded that SM causes a significant upregulation of aromatase gene expression in Sertoli cells, leading to a consequent increase in 17β-estradiol secretion. High level of 17β-estradiol in the testis could have potentially adverse effects on male fertility and testicular cancer.
format	article
author	Elisa Cirelli Emanuela De Domenico Flavia Botti Renato Massoud Raffaele Geremia Paola Grimaldi
author_facet	Elisa Cirelli Emanuela De Domenico Flavia Botti Renato Massoud Raffaele Geremia Paola Grimaldi
author_sort	Elisa Cirelli
title	Effect Of Microgravity On Aromatase Expression In Sertoli Cells
title_short	Effect Of Microgravity On Aromatase Expression In Sertoli Cells
title_full	Effect Of Microgravity On Aromatase Expression In Sertoli Cells
title_fullStr	Effect Of Microgravity On Aromatase Expression In Sertoli Cells
title_full_unstemmed	Effect Of Microgravity On Aromatase Expression In Sertoli Cells
title_sort	effect of microgravity on aromatase expression in sertoli cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c1201aea0a8247aaafdddcd61764f5e6
work_keys_str_mv	AT elisacirelli effectofmicrogravityonaromataseexpressioninsertolicells AT emanueladedomenico effectofmicrogravityonaromataseexpressioninsertolicells AT flaviabotti effectofmicrogravityonaromataseexpressioninsertolicells AT renatomassoud effectofmicrogravityonaromataseexpressioninsertolicells AT raffaelegeremia effectofmicrogravityonaromataseexpressioninsertolicells AT paolagrimaldi effectofmicrogravityonaromataseexpressioninsertolicells
_version_	1718384928564969472

Effect Of Microgravity On Aromatase Expression In Sertoli Cells

Ejemplares similares