Preparation and biological characterization of the mixture of poly(lactic-co-glycolic acid)/chitosan/Ag nanoparticles for periodontal tissue engineering

Yanxiang Xue,1,2 Xiaofang Hong,3 Jie Gao,1,2 Renze Shen,3 Zhanchao Ye3 1Department of Stomatology, The Liwan Hospital of The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510000, China; 2Department of Stomatology, Southern Medical University Guangzhou, Guangzhou 510515, China...

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Autores principales:	Xue Y, Hong X, Gao J, Shen R, Ye Z
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	nanoparticles bone regeneratio Periodontitis Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/c1209f42971943609d4117616eb51c7b
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spelling	oai:doaj.org-article:c1209f42971943609d4117616eb51c7b2021-12-02T01:06:02ZPreparation and biological characterization of the mixture of poly(lactic-co-glycolic acid)/chitosan/Ag nanoparticles for periodontal tissue engineering1178-2013https://doaj.org/article/c1209f42971943609d4117616eb51c7b2019-01-01T00:00:00Zhttps://www.dovepress.com/preparation-and-biological-characterization-of-the-mixture-of-polylact-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yanxiang Xue,1,2 Xiaofang Hong,3 Jie Gao,1,2 Renze Shen,3 Zhanchao Ye3 1Department of Stomatology, The Liwan Hospital of The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510000, China; 2Department of Stomatology, Southern Medical University Guangzhou, Guangzhou 510515, China; 3Department of Stomatology, Zhongshan Hospital of Xiamen University, Medical College of Xiamen University, Xiamen University, Xiamen 361000, China Objective: This study aims to produce nanoparticles of chitosan (CS), poly(lactic-co-glycolic acid) (PLGA), and silver and investigate the optimal composite ratio of these three materials for periodontal tissue regeneration.Methods: PLGA nanoparticles (nPLGA), CS nanoparticles (nCS), and silver nanoparticles (nAg) were prepared. The antibacterial properties of single nanoparticles and their effects on the proliferation and mineralization of periodontal membrane cells were investigated. Different ratios of nPLGA and nCS were combined, the proliferation and mineralization of periodontal membrane cells were investigated, and based on the results, the optimal ratio was determined. Finally, nPLGA and nCS in optimal ratio were combined with nAg, and the effects of the complex of these three materials on the proliferation and mineralization of periodontal membrane cells were investigated and tested in animals.Results: The single nanoparticles were found to have no cytotoxicity and were able to promote cell mineralization. nCS and nAg in low concentrations showed antibacterial activity; however, nAg inhibited cell proliferation. The nPLGA and nCS complex in 3:7 ratio contributed to cell mineralization and had no cytotoxicity. nPLGA/nCS/nAg complex, which had the optimal proportion of the three materials, showed no cytotoxicity and contributed to cell mineralization.Conclusion: nPLGA/nCS/nAg complex had no cytotoxicity and contributed to cell mineralization. The 3:7 ratio of nPLGA/nCS and 50 µg/mL nAg were found as the optimal proportion of the three materials. Keywords: nanoparticles, bone regeneration, periodontitisXue YHong XGao JShen RYe ZDove Medical Pressarticlenanoparticlesbone regeneratioPeriodontitisMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 483-498 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	nanoparticles bone regeneratio Periodontitis Medicine (General) R5-920
spellingShingle	nanoparticles bone regeneratio Periodontitis Medicine (General) R5-920 Xue Y Hong X Gao J Shen R Ye Z Preparation and biological characterization of the mixture of poly(lactic-co-glycolic acid)/chitosan/Ag nanoparticles for periodontal tissue engineering
description	Yanxiang Xue,1,2 Xiaofang Hong,3 Jie Gao,1,2 Renze Shen,3 Zhanchao Ye3 1Department of Stomatology, The Liwan Hospital of The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510000, China; 2Department of Stomatology, Southern Medical University Guangzhou, Guangzhou 510515, China; 3Department of Stomatology, Zhongshan Hospital of Xiamen University, Medical College of Xiamen University, Xiamen University, Xiamen 361000, China Objective: This study aims to produce nanoparticles of chitosan (CS), poly(lactic-co-glycolic acid) (PLGA), and silver and investigate the optimal composite ratio of these three materials for periodontal tissue regeneration.Methods: PLGA nanoparticles (nPLGA), CS nanoparticles (nCS), and silver nanoparticles (nAg) were prepared. The antibacterial properties of single nanoparticles and their effects on the proliferation and mineralization of periodontal membrane cells were investigated. Different ratios of nPLGA and nCS were combined, the proliferation and mineralization of periodontal membrane cells were investigated, and based on the results, the optimal ratio was determined. Finally, nPLGA and nCS in optimal ratio were combined with nAg, and the effects of the complex of these three materials on the proliferation and mineralization of periodontal membrane cells were investigated and tested in animals.Results: The single nanoparticles were found to have no cytotoxicity and were able to promote cell mineralization. nCS and nAg in low concentrations showed antibacterial activity; however, nAg inhibited cell proliferation. The nPLGA and nCS complex in 3:7 ratio contributed to cell mineralization and had no cytotoxicity. nPLGA/nCS/nAg complex, which had the optimal proportion of the three materials, showed no cytotoxicity and contributed to cell mineralization.Conclusion: nPLGA/nCS/nAg complex had no cytotoxicity and contributed to cell mineralization. The 3:7 ratio of nPLGA/nCS and 50 µg/mL nAg were found as the optimal proportion of the three materials. Keywords: nanoparticles, bone regeneration, periodontitis
format	article
author	Xue Y Hong X Gao J Shen R Ye Z
author_facet	Xue Y Hong X Gao J Shen R Ye Z
author_sort	Xue Y
title	Preparation and biological characterization of the mixture of poly(lactic-co-glycolic acid)/chitosan/Ag nanoparticles for periodontal tissue engineering
title_short	Preparation and biological characterization of the mixture of poly(lactic-co-glycolic acid)/chitosan/Ag nanoparticles for periodontal tissue engineering
title_full	Preparation and biological characterization of the mixture of poly(lactic-co-glycolic acid)/chitosan/Ag nanoparticles for periodontal tissue engineering
title_fullStr	Preparation and biological characterization of the mixture of poly(lactic-co-glycolic acid)/chitosan/Ag nanoparticles for periodontal tissue engineering
title_full_unstemmed	Preparation and biological characterization of the mixture of poly(lactic-co-glycolic acid)/chitosan/Ag nanoparticles for periodontal tissue engineering
title_sort	preparation and biological characterization of the mixture of poly(lactic-co-glycolic acid)/chitosan/ag nanoparticles for periodontal tissue engineering
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/c1209f42971943609d4117616eb51c7b
work_keys_str_mv	AT xuey preparationandbiologicalcharacterizationofthemixtureofpolylacticcoglycolicacidchitosanagnanoparticlesforperiodontaltissueengineering AT hongx preparationandbiologicalcharacterizationofthemixtureofpolylacticcoglycolicacidchitosanagnanoparticlesforperiodontaltissueengineering AT gaoj preparationandbiologicalcharacterizationofthemixtureofpolylacticcoglycolicacidchitosanagnanoparticlesforperiodontaltissueengineering AT shenr preparationandbiologicalcharacterizationofthemixtureofpolylacticcoglycolicacidchitosanagnanoparticlesforperiodontaltissueengineering AT yez preparationandbiologicalcharacterizationofthemixtureofpolylacticcoglycolicacidchitosanagnanoparticlesforperiodontaltissueengineering
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Preparation and biological characterization of the mixture of poly(lactic-co-glycolic acid)/chitosan/Ag nanoparticles for periodontal tissue engineering

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