Biochemical, stabilization and crystallization studies on a molecular chaperone (PaoD) involved in the maturation of molybdoenzymes.

Molybdenum and tungsten enzymes require specific chaperones for folding and cofactor insertion. PaoD is the chaperone of the periplasmic aldehyde oxidoreductase PaoABC. It is the last gene in the paoABCD operon in Escherichia coli and its presence is crucial for obtaining mature enzyme. PaoD is an u...

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Autores principales: Ana Rita Otrelo-Cardoso, Viola Schwuchow, David Rodrigues, Eurico J Cabrita, Silke Leimkühler, Maria João Romão, Teresa Santos-Silva
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2014
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Acceso en línea:https://doaj.org/article/c1261c4a38884b709db8fd5c8cb646fa
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