A human ESC line for efficient CRISPR editing of pluripotent stem cells

Human pluripotent stem cells (hPSC) can be directed to differentiate in vitro into insulin-prorducing beta cells (SC-β). Although these cells accurately respond to glucose and can reverse diabetes in preclinical models improvments in the final cell products are desirable. For example, safety, contro...

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Autores principales:	Elad Sintov, Dario Gerace, Douglas A. Melton
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/c13124af03dd436c9fac4750b2a61845
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spelling	oai:doaj.org-article:c13124af03dd436c9fac4750b2a618452021-11-04T04:27:42ZA human ESC line for efficient CRISPR editing of pluripotent stem cells1873-506110.1016/j.scr.2021.102591https://doaj.org/article/c13124af03dd436c9fac4750b2a618452021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1873506121004384https://doaj.org/toc/1873-5061Human pluripotent stem cells (hPSC) can be directed to differentiate in vitro into insulin-prorducing beta cells (SC-β). Although these cells accurately respond to glucose and can reverse diabetes in preclinical models improvments in the final cell products are desirable. For example, safety, controlling the cellular compositions and protection against immune rejection may be addressed by genetic modifications of SC-β pre-transplantation. To screen for gene targets, we have generated a human embryonic stem cell line (hESC) that constitutively express the enhanced specificity Streptococcus pyogenes Cas9 (eSpCas9) gene, knocked-in into the GAPDH locus.Elad SintovDario GeraceDouglas A. MeltonElsevierarticleBiology (General)QH301-705.5ENStem Cell Research, Vol 57, Iss , Pp 102591- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Elad Sintov Dario Gerace Douglas A. Melton A human ESC line for efficient CRISPR editing of pluripotent stem cells
description	Human pluripotent stem cells (hPSC) can be directed to differentiate in vitro into insulin-prorducing beta cells (SC-β). Although these cells accurately respond to glucose and can reverse diabetes in preclinical models improvments in the final cell products are desirable. For example, safety, controlling the cellular compositions and protection against immune rejection may be addressed by genetic modifications of SC-β pre-transplantation. To screen for gene targets, we have generated a human embryonic stem cell line (hESC) that constitutively express the enhanced specificity Streptococcus pyogenes Cas9 (eSpCas9) gene, knocked-in into the GAPDH locus.
format	article
author	Elad Sintov Dario Gerace Douglas A. Melton
author_facet	Elad Sintov Dario Gerace Douglas A. Melton
author_sort	Elad Sintov
title	A human ESC line for efficient CRISPR editing of pluripotent stem cells
title_short	A human ESC line for efficient CRISPR editing of pluripotent stem cells
title_full	A human ESC line for efficient CRISPR editing of pluripotent stem cells
title_fullStr	A human ESC line for efficient CRISPR editing of pluripotent stem cells
title_full_unstemmed	A human ESC line for efficient CRISPR editing of pluripotent stem cells
title_sort	human esc line for efficient crispr editing of pluripotent stem cells
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/c13124af03dd436c9fac4750b2a61845
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A human ESC line for efficient CRISPR editing of pluripotent stem cells

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