Rapid MALDI-TOF MS identification of commercial truffles

Abstract Truffles are edible mushrooms with similar morphological characteristics, that make it difficult to distinguish between highly prized truffles (such as the Périgord black T. melanosporum) and inexpensive truffles (such as the Asian Black T. indicum). These biological and economic features h...

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Autores principales:	Khalid El Karkouri, Carine Couderc, Philippe Decloquement, Annick Abeille, Didier Raoult
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c134b9b320fc4c6abd0b05caad8add66
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spelling	oai:doaj.org-article:c134b9b320fc4c6abd0b05caad8add662021-12-02T15:08:08ZRapid MALDI-TOF MS identification of commercial truffles10.1038/s41598-019-54214-x2045-2322https://doaj.org/article/c134b9b320fc4c6abd0b05caad8add662019-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-54214-xhttps://doaj.org/toc/2045-2322Abstract Truffles are edible mushrooms with similar morphological characteristics, that make it difficult to distinguish between highly prized truffles (such as the Périgord black T. melanosporum) and inexpensive truffles (such as the Asian Black T. indicum). These biological and economic features have led to several misidentifications and/or fraudulent profit in the truffle markets. In this paper, we investigate Matrix-assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) biotyping to identify 34 commercial fresh truffles from Europe and Asia. The MALDI-TOF MS clustering rapidly distinguished seven Tuber species identified by ITS phylogenetic analysis. The tasty T. melanosporum was clearly differentiated from the Chinese and less expensive truffles. These cheaper mushrooms were marketed as T. indicum but corresponded to a mix of three species. In total, the method confirmed misidentifications in 26% of commercial specimens. Several unknown blind-coded truffles were rapidly identified, with scores >= 2, using the Bruker Biotyper algorithm against MS databases. This study demonstrates that MALDI-TOF MS is a reliable, rapid and cheaper new tool compared with molecular methods for the identification of truffle species and could be used to control frauds in the truffle markets. It could also be useful for the certification of truffle-inoculated seedlings and/or diversity in forest ecosystems.Khalid El KarkouriCarine CoudercPhilippe DecloquementAnnick AbeilleDidier RaoultNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-9 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Khalid El Karkouri Carine Couderc Philippe Decloquement Annick Abeille Didier Raoult Rapid MALDI-TOF MS identification of commercial truffles
description	Abstract Truffles are edible mushrooms with similar morphological characteristics, that make it difficult to distinguish between highly prized truffles (such as the Périgord black T. melanosporum) and inexpensive truffles (such as the Asian Black T. indicum). These biological and economic features have led to several misidentifications and/or fraudulent profit in the truffle markets. In this paper, we investigate Matrix-assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) biotyping to identify 34 commercial fresh truffles from Europe and Asia. The MALDI-TOF MS clustering rapidly distinguished seven Tuber species identified by ITS phylogenetic analysis. The tasty T. melanosporum was clearly differentiated from the Chinese and less expensive truffles. These cheaper mushrooms were marketed as T. indicum but corresponded to a mix of three species. In total, the method confirmed misidentifications in 26% of commercial specimens. Several unknown blind-coded truffles were rapidly identified, with scores >= 2, using the Bruker Biotyper algorithm against MS databases. This study demonstrates that MALDI-TOF MS is a reliable, rapid and cheaper new tool compared with molecular methods for the identification of truffle species and could be used to control frauds in the truffle markets. It could also be useful for the certification of truffle-inoculated seedlings and/or diversity in forest ecosystems.
format	article
author	Khalid El Karkouri Carine Couderc Philippe Decloquement Annick Abeille Didier Raoult
author_facet	Khalid El Karkouri Carine Couderc Philippe Decloquement Annick Abeille Didier Raoult
author_sort	Khalid El Karkouri
title	Rapid MALDI-TOF MS identification of commercial truffles
title_short	Rapid MALDI-TOF MS identification of commercial truffles
title_full	Rapid MALDI-TOF MS identification of commercial truffles
title_fullStr	Rapid MALDI-TOF MS identification of commercial truffles
title_full_unstemmed	Rapid MALDI-TOF MS identification of commercial truffles
title_sort	rapid maldi-tof ms identification of commercial truffles
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/c134b9b320fc4c6abd0b05caad8add66
work_keys_str_mv	AT khalidelkarkouri rapidmalditofmsidentificationofcommercialtruffles AT carinecouderc rapidmalditofmsidentificationofcommercialtruffles AT philippedecloquement rapidmalditofmsidentificationofcommercialtruffles AT annickabeille rapidmalditofmsidentificationofcommercialtruffles AT didierraoult rapidmalditofmsidentificationofcommercialtruffles
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Rapid MALDI-TOF MS identification of commercial truffles

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